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| 2. |  | MARTINS, F. A.; CARNEIRO, P. C. S.; CRUZ, C. D.; CARNEIRO, J. E. de S.; GUIMARAES, C. T. Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 10, p. 1291-1296, out. 2009.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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| 4. | | SILVA, J. N. M.; LOPES, J. do C. A.; OLIVEIRA, L. C. de; SILVA, S. M. A. da; CARVALHO, J. O. P. de; COSTA, D. H. M.; MELO, M. S.; TAVARES, M. J. M. Diretrizes para instalação e medição de parcelas permanentes em florestas naturais da Amazônia brasileira. Belém, PA: Embrapa Amazônia Oriental, 2005. 68 p.| Biblioteca(s): Embrapa Acre; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental. |
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| Registros recuperados : 6 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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Data corrente: |
07/01/2010 |
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Data da última atualização: |
25/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MARTINS, F. A.; CARNEIRO, P. C. S.; CRUZ, C. D.; CARNEIRO, J. E. de S.; GUIMARAES, C. T. |
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Afiliação: |
FRANCIELLE ALLINE MARTINS, UFV; PEDRO CRESCÊNCIO SOUZA CARNEIRO, UFV; COSME DAMIÃO CRUZ, UFV; JOSÉ EUSTÁQUIO DE SOUZA CARNEIRO, UFV; CLAUDIA TEIXEIRA GUIMARAES, CNPMS. |
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Título: |
Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 10, p. 1291-1296, out. 2009. |
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DOI: |
10.1590/S0100-204X2009001000012 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations. |
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Palavras-Chave: |
Allelic origin; Heterozigoto; Heterozygote; Natural populations; Origem alélica; PCR semiquantitativa; Poligenes; Polygenes; Populações naturais; Semiquantitative PCR. |
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Thesagro: |
Zea Mays. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160668/1/Endosperm-genotyping.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE-2010/47247/1/44n10a12.pdf
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Marc: |
LEADER 01894naa a2200313 a 4500
001 1580267
005 2018-05-25
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1590/S0100-204X2009001000012$2DOI
100 1 $aMARTINS, F. A.
245 $aEndosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny.
260 $c2009
520 $aThe objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations.
650 $aZea Mays
653 $aAllelic origin
653 $aHeterozigoto
653 $aHeterozygote
653 $aNatural populations
653 $aOrigem alélica
653 $aPCR semiquantitativa
653 $aPoligenes
653 $aPolygenes
653 $aPopulações naturais
653 $aSemiquantitative PCR
700 1 $aCARNEIRO, P. C. S.
700 1 $aCRUZ, C. D.
700 1 $aCARNEIRO, J. E. de S.
700 1 $aGUIMARAES, C. T.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 44, n. 10, p. 1291-1296, out. 2009.
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Embrapa Milho e Sorgo (CNPMS) |
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