01894naa a2200313 a 450000100080000000500110000800800410001902400410006010000190010124501050012026000090022552009530023465000130118765300190120065300170121965300170123665300240125365300200127765300250129765300140132265300140133665300260135065300250137670000230140170000160142470000260144070000210146677300930148715802672018-05-25       2009    bl uuuu     u00u1 u    #d7 a10.1590/S0100-204X20090010000122DOI1 aMARTINS, F. A.  aEndosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny.  c2009  aThe objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations.  aZea Mays  aAllelic origin  aHeterozigoto  aHeterozygote  aNatural populations  aOrigem alélica  aPCR semiquantitativa  aPoligenes  aPolygenes  aPopulações naturais  aSemiquantitative PCR1 aCARNEIRO, P. C. S.1 aCRUZ, C. D.1 aCARNEIRO, J. E. de S.1 aGUIMARAES, C. T.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 44, n. 10, p. 1291-1296, out. 2009.