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| 2. |  | FRASCA-SCORVO, C. M. D.; LOSEKANN, M. E.; SCORVO FILHO, J. D.; CESNIK, R.; QUEIROZ, J. F. de; RIBEIRO, M. A. G.; ALVES, J. M. C.; DONADELLI, A.; TURCO, P. H. N. Avaliação do cultivo de tilápias em tanques-rede em uma represa rural: efeito da densidade no desempenho zootécnico. In: CONGRESSO DE AQUACIÊNCIA, 2008, Maringá. Desafios e inovação: resumos. Maringá: Aquabio:: UEM, 2008. 1p. CD-ROM.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 3. | | CARVALHO, H. W. L. de; CARDOSO, M. J.; ROCHA, L. M. P.; PACHECO, C. A. P.; OLIVEIRA, I. R. de; TABOSA, J. N.; LIRA, M. A.; OLIVEIRA, E. A. S.; NASCIMENTO, M. M. A. do; SIMPLÍCIO, J. B.; COUTINHO, G. V.; BRITO, A. R. de M. B.; TAVARES, J. A.; TAVARES FILHO, J. J.; MELO, K. E. de O.; FEITOSA, L. F.; MENEZES, A. F.; RODRIGUES, C. S.; SILVA, B. S. de F. Desempenho de híbridos de milho no nordeste brasileiro: safra 2006/2007. Aracaju: Embrapa Tabuleiros Costeiros, 2008. 8 p. (Embrapa Tabuleiros Costeiros. Comunicado Técnico, 75).| Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Algodão; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Meio Norte / UEP-Parnaíba; Embrapa Meio-Norte; Embrapa Pecuária Sudeste; Embrapa Rondônia; Embrapa Roraima; Embrapa Semiárido; Embrapa Tabuleiros Costeiros; Embrapa Unidades Centrais. MenosEmbrapa Agropecuária Oeste; Embrapa Algodão; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Meio Norte / UEP-Parnaíba; Embrapa Meio-Norte; Embrapa Pecuária Sudeste; Embrapa Rondônia; Embrapa Roraima... Mostrar Todas |
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| 10. | | UCHÔA, S. C. P.; NASCIMENTO, F. R. do; ALVES, J. M. A.; MELO, V. F.; SILVA, D. C. O. da; SILVA, A. J. da; BATISTA, K. D.; MATOS, K. da S.; ALBUQUERQUE, J. de A. A. de. Adubação fosfatada na produtividade e qualidade de raízes tuberosas de cultivares de mandioca na savana amazônica, Brasil. Revista de Ciências Agrárias, v. 43, n.4, p. 381-389, 2020.| Biblioteca(s): Embrapa Roraima. |
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| 11. | | MONTEIRO, C. M. O.; CESTARO, L.; FAZA, A. P.; PRATA, M. C. de A.; DOLINSKI, C.; FURLONG, J. Ação de Heterorhabditis bacteriophora (Rhabditida: heterorhabditidae), isolado HP88 no controle de Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). In: SEMANA DE BIOLOGIA, 31., MOSTRA DE PRODUÇÃO CIENTÍFICA. 14., 2008, Juiz de Fora. Anais... Juiz de Fora: UFJF, 2008. 4 p. 1 CD.| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | LIBÓRIO, C. B.; VERZIGNASSI, J. R.; PEREIRA, F. A. R.; FERNANDES, C. D.; MONTEIRO, L. C.; BENTEO, G. L.; LIMA, N. D.; CARDOSO, L. C. M. C.; JESUS, L. de; CORADO, H. S. Dessecação de genótipos de Brachiaria por glifosato. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 10., 2014, Campo Grande, MS. [Anais da..]. Campo Grande, MS: Embrapa Gado de Corte, 2014. p. 28-29. 2 p. (Embrapa Gado de Corte. Documentos, 208). Comissão organizadora: Grácia Maria Soares Rosinha, Alexandra Rocha de Oliveir, Rodrigo Carvalho Alva.| Biblioteca(s): Embrapa Gado de Corte. |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
26/07/2018 |
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Data da última atualização: |
26/07/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CARVALHO, A. L. de A.; REZENDE, L. C. de; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; PINTO, Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V.; BETTIOL, W. |
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Afiliação: |
ANDRE LUIZ DE ANDRADE CARVALHO, UFV; LARISSA CASTRO DE REZENDE, UFV; LUCIO BERTOLDO COSTA, FCA-UNESP; BERNARDO DE ALMEIDA HALFELD VIEIRA, CNPMA; ZAYAME VEGETTE PINTO, FCA-UNESP; MARCELO AUGUSTO BOECHAT MORANDI, CNPMA; FLAVIO HENRIQUE VASCONCELOS DE MEDEIROS, UFV; WAGNER BETTIOL, CNPMA. |
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Título: |
Optimizing the mass production of Clonostachys rosea by liquid-state fermentation. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Biological Control, v. 118, p. 16-25, 2018. |
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DOI: |
https://doi.org/10.1016/j.biocontrol.2017.11.014 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions that resulted in a maximum yield of conidia (1.78 x 107 conidia?mL-1), dried biomass (0.558?g) and CFUs (5.15 x 106 CFUs?mL-1) were a pH value of 4 and a photoperiod of 12?h. MenosAbstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions th... Mostrar Tudo |
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Palavras-Chave: |
Bioreactor; Liquid media; Produção massal. |
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Thesagro: |
Fungo Para Controle Biológico. |
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Thesaurus NAL: |
Biological control agents; Biomass production; Bioreactors; Clonostachys rosea. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
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100 1 $aCARVALHO, A. L. de A.
245 $aOptimizing the mass production of Clonostachys rosea by liquid-state fermentation.$h[electronic resource]
260 $c2018
520 $aAbstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions that resulted in a maximum yield of conidia (1.78 x 107 conidia?mL-1), dried biomass (0.558?g) and CFUs (5.15 x 106 CFUs?mL-1) were a pH value of 4 and a photoperiod of 12?h.
650 $aBiological control agents
650 $aBiomass production
650 $aBioreactors
650 $aClonostachys rosea
650 $aFungo Para Controle Biológico
653 $aBioreactor
653 $aLiquid media
653 $aProdução massal
700 1 $aREZENDE, L. C. de
700 1 $aCOSTA, L. B.
700 1 $aHALFELD-VIEIRA, B. de A.
700 1 $aPINTO, Z. V.
700 1 $aMORANDI, M. A. B.
700 1 $aMEDEIROS, F. H. V.
700 1 $aBETTIOL, W.
773 $tBiological Control$gv. 118, p. 16-25, 2018.
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