02628naa a2200313 a 450000100080000000500110000800800410001902400580006010000260011824501100014426000090025452016760026365000300193965000230196965000160199265000230200865000350203165300150206665300170208165300220209870000220212070000170214270000290215970000170218870000220220570000230222770000160225077300480226620935872018-07-26       2018    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1016/j.biocontrol.2017.11.0142DOI1 aCARVALHO, A. L. de A.  aOptimizing the mass production of Clonostachys rosea by liquid-state fermentation.h[electronic resource]  c2018  aAbstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions that resulted in a maximum yield of conidia (1.78 x 107 conidia?mL-1), dried biomass (0.558?g) and CFUs (5.15 x 106 CFUs?mL-1) were a pH value of 4 and a photoperiod of 12?h.  aBiological control agents  aBiomass production  aBioreactors  aClonostachys rosea  aFungo Para Controle Biológico  aBioreactor  aLiquid media  aProdução massal1 aREZENDE, L. C. de1 aCOSTA, L. B.1 aHALFELD-VIEIRA, B. de A.1 aPINTO, Z. V.1 aMORANDI, M. A. B.1 aMEDEIROS, F. H. V.1 aBETTIOL, W.  tBiological Controlgv. 118, p. 16-25, 2018.