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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Meio-Norte. |
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Data corrente: |
11/08/2014 |
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Data da última atualização: |
25/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RAPOSO, R. S.; SOUZA, I. G. B.; VELOSO, M. E. da C.; KOBAYASHI, A. K.; LAVIOLA, B. G.; DINIZ, F. M. |
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Afiliação: |
R.S. Raposo, Universidade Federal do Ceara, Núcleo de Biologia Experimental.; I.G.B. Souza, Universidade Federal do Piauí.; MARCOS EMANUEL DA COSTA VELOSO, CPAMN; ADILSON KENJI KOBAYASHI, CNPAE; BRUNO GALVEAS LAVIOLA, CNPAE; FABIO MENDONCA DINIZ, CPAMN. |
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Título: |
Development of novel simple sequence repeat markers from a genomic sequence survey database and their application for diversity assessment in Jatropha curcas germplasm from Guatemala. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 3, p. 6099-6106, Aug. 2014. |
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DOI: |
http://dx.doi.org/10.4238/2014.August.7.25 |
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Idioma: |
Inglês |
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Conteúdo: |
The last few years have seen a significant increase in the number of large-scale sequencing projects generating whole genome databases. These sequence databases can be surveyed (genome sequence survey) for tandem repeats as an alternative means to develop microsatellites for monitoring and selecting natural populations and cultivars of Jatropha curcas. A total of 100 tandem repeats were revealed from mining 368 genomic surveyed sequences available in the Kazusa DNA Research Institute database. Twenty microsatellite sequences were successfully amplified, resulting in repeatable and scorable polymerase chain reaction products. Genotyping of J. curcas accessions from the Guatemalan population revealed 18 polymorphic loci. The average number of alleles per locus was 6.9, and allelic sizes ranged from 94 to 299 bp. Expected and observed heterozygosities ranged from 0.118 to 0.906 and from 0.082 to 0.794, respectively. Polymorphic information content values ranged from 0.114 (JcSSR-34) to 0.886 (JcSSR-33) with an average of 0.627. Analysis with Micro-Checker indicated few null alleles for locus JcSSR-37 in Guatemalan populations, which may be a possible cause of its deviation from Hardy-Weinberg equilibrium, even after Bonferroni?s correction. No loci showed significant linkage disequilibrium. These microsatellite loci are expected to be valuable molecular markers in J. curcas because they show high levels of polymorphism and heterozygosity. |
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Palavras-Chave: |
Biofuel; Energy crop; Genetic diversity; Microsatellites; Microssatélite; Physic nut. |
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Thesagro: |
Biocombustível; Cultura energética; Euphorbiaceae; Pinhão de purga; Planta oleaginosa. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/125432/1/ArtigoMarcosFabioGMR2014.pdf
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Marc: |
LEADER 02543naa a2200325 a 4500
001 2017730
005 2022-05-25
008 2014 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/2014.August.7.25$2DOI
100 1 $aRAPOSO, R. S.
245 $aDevelopment of novel simple sequence repeat markers from a genomic sequence survey database and their application for diversity assessment in Jatropha curcas germplasm from Guatemala.$h[electronic resource]
260 $c2014
520 $aThe last few years have seen a significant increase in the number of large-scale sequencing projects generating whole genome databases. These sequence databases can be surveyed (genome sequence survey) for tandem repeats as an alternative means to develop microsatellites for monitoring and selecting natural populations and cultivars of Jatropha curcas. A total of 100 tandem repeats were revealed from mining 368 genomic surveyed sequences available in the Kazusa DNA Research Institute database. Twenty microsatellite sequences were successfully amplified, resulting in repeatable and scorable polymerase chain reaction products. Genotyping of J. curcas accessions from the Guatemalan population revealed 18 polymorphic loci. The average number of alleles per locus was 6.9, and allelic sizes ranged from 94 to 299 bp. Expected and observed heterozygosities ranged from 0.118 to 0.906 and from 0.082 to 0.794, respectively. Polymorphic information content values ranged from 0.114 (JcSSR-34) to 0.886 (JcSSR-33) with an average of 0.627. Analysis with Micro-Checker indicated few null alleles for locus JcSSR-37 in Guatemalan populations, which may be a possible cause of its deviation from Hardy-Weinberg equilibrium, even after Bonferroni?s correction. No loci showed significant linkage disequilibrium. These microsatellite loci are expected to be valuable molecular markers in J. curcas because they show high levels of polymorphism and heterozygosity.
650 $aBiocombustível
650 $aCultura energética
650 $aEuphorbiaceae
650 $aPinhão de purga
650 $aPlanta oleaginosa
653 $aBiofuel
653 $aEnergy crop
653 $aGenetic diversity
653 $aMicrosatellites
653 $aMicrossatélite
653 $aPhysic nut
700 1 $aSOUZA, I. G. B.
700 1 $aVELOSO, M. E. da C.
700 1 $aKOBAYASHI, A. K.
700 1 $aLAVIOLA, B. G.
700 1 $aDINIZ, F. M.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 13, n. 3, p. 6099-6106, Aug. 2014.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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Origem |
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Classificação |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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Data corrente: |
09/02/2018 |
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Data da última atualização: |
18/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SIHLER, W.; SOUZA, M. L. de; VALICENTE, F. H.; FALCAO, R.; SANCHES, M. M. |
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Afiliação: |
WILLIAM SIHLER, Cenargen; MARLINDA LOBO DE SOUZA, Cenargen; FERNANDO HERCOS VALICENTE, CNPMS; ROSANA FALCAO, CNPAE; MARCIO MARTINELLO SANCHES, Cenargen. |
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Título: |
In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, v. 53, n. 1, p. 1-9, 2018. |
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DOI: |
10.1590/S0100-204X2018000100001 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTITn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDSPage of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV. |
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Palavras-Chave: |
Cultura de células; Lagarta-do-cartucho; Produção in vitro; SfMNPV. |
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Thesagro: |
Baculovirus; Controle biológico; Spodoptera Frugiperda. |
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Thesaurus NAL: |
Baculoviridae; Biological control; Cell culture; Spodoptera frugiperda multiple nucleopolyhedrovirus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/172484/1/53n01a01.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/173947/1/In-vitro-infectivity-of-Spodoptera-frugiperda.pdf
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Marc: |
LEADER 02250naa a2200313 a 4500
001 2089326
005 2019-02-18
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1590/S0100-204X2018000100001$2DOI
100 1 $aSIHLER, W.
245 $aIn vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines.$h[electronic resource]
260 $c2018
520 $aThe objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTITn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDSPage of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV.
650 $aBaculoviridae
650 $aBiological control
650 $aCell culture
650 $aSpodoptera frugiperda multiple nucleopolyhedrovirus
650 $aBaculovirus
650 $aControle biológico
650 $aSpodoptera Frugiperda
653 $aCultura de células
653 $aLagarta-do-cartucho
653 $aProdução in vitro
653 $aSfMNPV
700 1 $aSOUZA, M. L. de
700 1 $aVALICENTE, F. H.
700 1 $aFALCAO, R.
700 1 $aSANCHES, M. M.
773 $tPesquisa Agropecuária Brasileira, Brasília$gv. 53, n. 1, p. 1-9, 2018.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Origem |
Tipo/Formato |
Classificação |
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