02250naa a2200313 a 450000100080000000500110000800800410001902400410006010000150010124501350011626000090025152012610026065000180152165000230153965000170156265000560157965000160163565000240165165000260167565300240170165300240172565300240174965300110177370000200178470000210180470000150182570000190184077300770185920893262019-02-18       2018    bl uuuu     u00u1 u    #d7 a10.1590/S0100-204X20180001000012DOI1 aSIHLER, W.  aIn vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines.h[electronic resource]  c2018  aThe objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTITn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell lines showed hypertrophy of cell nuclei and production of many polyhedra. The SDSPage of radiolabed proteins showed that the cell protein synthesis was shutoff, while an intense band of about 30 kDa, recognized as polyhedrin, was synthesized. The other cell lines did not show polyhedra production, although some of them underwent morphological changes and protein synthesis shutdown in response to virus infection. The SF-21 and Sf9 cell lines are recommended for further in vitro production of SfMNPV.  aBaculoviridae  aBiological control  aCell culture  aSpodoptera frugiperda multiple nucleopolyhedrovirus  aBaculovirus  aControle biológico  aSpodoptera Frugiperda  aCultura de células  aLagarta-do-cartucho  aProdução in vitro  aSfMNPV1 aSOUZA, M. L. de1 aVALICENTE, F. H.1 aFALCAO, R.1 aSANCHES, M. M.  tPesquisa Agropecuária Brasileira, Brasíliagv. 53, n. 1, p. 1-9, 2018.