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Registro Completo |
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
23/11/2012 |
Data da última atualização: |
30/11/2012 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FERNANDES, F. S.; RAMALHO, F. de S.; MALAQUIAS, J. B.; NASCIMENTO JUNIOR, J. L.; CORREIA, E. T.; ZANUNCIO, J. C. |
Afiliação: |
FRANCISCO DE SOUZA RAMALHO, CNPA; JOSE BRUNO MALAQUIAS, CNPA. |
Título: |
Within-plant distribution of cotton aphid (hemiptera: Aphididae) in cotton cultivars with colored fibers. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Anais da Academia Brasileira de Ciências, 2012. |
Volume: |
84 |
Páginas: |
p. 245-261 |
ISSN: |
0001-3765 |
Idioma: |
Português |
Palavras-Chave: |
FIBRAS COLORIDAS. |
Thesagro: |
Algodão. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/71187/1/scielo.php.htm
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Marc: |
LEADER 00690naa a2200229 a 4500 001 1940464 005 2012-11-30 008 2012 bl uuuu u00u1 u #d 022 $a0001-3765 100 1 $aFERNANDES, F. S. 245 $aWithin-plant distribution of cotton aphid (hemiptera$bAphididae) in cotton cultivars with colored fibers.$h[electronic resource] 260 $c2012 300 $ap. 245-261 84 490 $v84 650 $aAlgodão 653 $aFIBRAS COLORIDAS 700 1 $aRAMALHO, F. de S. 700 1 $aMALAQUIAS, J. B. 700 1 $aNASCIMENTO JUNIOR, J. L. 700 1 $aCORREIA, E. T. 700 1 $aZANUNCIO, J. C. 773 $tAnais da Academia Brasileira de Ciências, 2012.
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Registro original: |
Embrapa Algodão (CNPA) |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
28/09/2016 |
Data da última atualização: |
24/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SILVA, C. R. C.; MONNERAT, R.; LIMA, L. M. de; MARTINS, E. S.; MELO FILHO, P. A.; PINHEIRO, M. P. N.; SANTOS, R. C. |
Afiliação: |
CARLIANE R. C. SILVA, UFRPE; ROSE GOMES MONNERAT SOLON DE PONTES, CENARGEN; LIZIANE MARIA DE LIMA, CNPA; ÉRICA S. MARTINS, CENARGEN; PÉRICLES A. MELO FIILHO, UFRPE; MORGANNA P. N. PINHEIRO, UFRPE; ROSEANE CAVALCANTI DOS SANTOS, CNPA. |
Título: |
Stable integration and expression of a cry1la gene conferring resistance to fall armyworm and boll weevil in cotton plants. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pest Management Science, v. 72, p. 1549-1557, 2016. |
Idioma: |
Inglês |
Conteúdo: |
BACKGROUND: Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by usingmolecular tools. RESULTS: Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (T0-34) containing one copy of cry1Ia detected by Southern blot. Protein was expressed in high concentration at 45 days after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of cry1Ia antibody was found in the midgut of boll weevils fed on T0-34 buds in an immunodetection assay. CONCLUSION: The gene introduced into plants confers resistance to boll weevil and fall armyworm. Transmission of the transgene occurred normally to T1 progeny.All plants showed phenotypically normal growth,with fertile flowers and abundant seeds. |
Palavras-Chave: |
Chemical inseticides. |
Thesagro: |
Anthonomus grandis; Gossypium hirsutum; Spodoptera frugiperda. |
Thesaurus NAL: |
cotton. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183293/1/Silva-et-al-2016-Pest-Management-Science.pdf
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Marc: |
LEADER 02157naa a2200253 a 4500 001 2053604 005 2023-03-24 008 2016 bl uuuu u00u1 u #d 100 1 $aSILVA, C. R. C. 245 $aStable integration and expression of a cry1la gene conferring resistance to fall armyworm and boll weevil in cotton plants.$h[electronic resource] 260 $c2016 520 $aBACKGROUND: Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by usingmolecular tools. RESULTS: Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (T0-34) containing one copy of cry1Ia detected by Southern blot. Protein was expressed in high concentration at 45 days after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of cry1Ia antibody was found in the midgut of boll weevils fed on T0-34 buds in an immunodetection assay. CONCLUSION: The gene introduced into plants confers resistance to boll weevil and fall armyworm. Transmission of the transgene occurred normally to T1 progeny.All plants showed phenotypically normal growth,with fertile flowers and abundant seeds. 650 $acotton 650 $aAnthonomus grandis 650 $aGossypium hirsutum 650 $aSpodoptera frugiperda 653 $aChemical inseticides 700 1 $aMONNERAT, R. 700 1 $aLIMA, L. M. de 700 1 $aMARTINS, E. S. 700 1 $aMELO FILHO, P. A. 700 1 $aPINHEIRO, M. P. N. 700 1 $aSANTOS, R. C. 773 $tPest Management Science$gv. 72, p. 1549-1557, 2016.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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