02157naa a2200253 a 450000100080000000500110000800800410001910000200006024501510008026000090023152013810024065000110162165000230163265000230165565000260167865300250170470000170172970000190174670000190176570000220178470000230180670000180182977300560184720536042023-03-24       2016    bl uuuu     u00u1 u    #d1 aSILVA, C. R. C.  aStable integration and expression of a cry1la gene conferring resistance to fall armyworm and boll weevil in cotton plants.h[electronic resource]  c2016  aBACKGROUND: Boll weevil is a serious pest of cotton crop. Effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. The current genetically modified Bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. This work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by usingmolecular tools. RESULTS: Four Brazilian cotton cultivars were microinjected with a minimal linear cassette generating 1248 putative lines. Complete gene integration was found in only one line (T0-34) containing one copy of cry1Ia detected by Southern blot. Protein was expressed in high concentration at 45 days after emergence (dae), decreasing by approximately 50% at 90 dae. Toxicity of the cry protein was demonstrated in feeding bioassays revealing 56.7% mortality to boll weevil fed buds and 88.1% mortality to fall armyworm fed leaves. A binding of cry1Ia antibody was found in the midgut of boll weevils fed on T0-34 buds in an immunodetection assay. CONCLUSION: The gene introduced into plants confers resistance to boll weevil and fall armyworm. Transmission of the transgene occurred normally to T1 progeny.All plants showed phenotypically normal growth,with fertile flowers and abundant seeds.  acotton  aAnthonomus grandis  aGossypium hirsutum  aSpodoptera frugiperda  aChemical inseticides1 aMONNERAT, R.1 aLIMA, L. M. de1 aMARTINS, E. S.1 aMELO FILHO, P. A.1 aPINHEIRO, M. P. N.1 aSANTOS, R. C.  tPest Management Sciencegv. 72, p. 1549-1557, 2016.