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| Registros recuperados : 5.205 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
07/08/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
DANIEL, A. G. S.; SATO, J. P. H.; GABARDO, M. P.; RESENDE, T. P.; BARCELLOS, D. E. S. N. de; PEREIRA, C. E. R.; VANNUCCI, F. A.; GUEDES, R. M. C. |
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Afiliação: |
Amanda G. S. Daniel, Departamento de Clínica e Cirurgia Veterinária/Escola de Veterinária/ Universidade Federal de Minas Gerais; José P. H. Sato, Departamento de Clínica e Cirurgia Veterinária/Escola de Veterinária/ Universidade Federal de Minas Gerais; Michelle P. Gabardo, Departamento de Clínica e Cirurgia Veterinária/Escola de Veterinária/ Universidade Federal de Minas Gerais; Talita P. Resende, Departamento de Clínica e Cirurgia Veterinária/Escola de Veterinária/ Universidade Federal de Minas Gerais; David E. S. N. de Barcellos, Setor de Suínos/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul; Carlos E. R. Pereira, Departamento de Clínica e Cirurgia Veterinária/Escola de Veterinária/ Universidade Federal de Minas Gerais; Fábio A. Vannucci, Microvet-Microbiologia Veterinária Especial. Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota; Roberto M.C. Guedes, Departamento de Clínica e Cirurgia Veterinária/Escola de Veterinária/ Universidade Federal de Minas Gerais. |
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Título: |
Minimum inhibitory concentration of Brazilian Brachyspira hyodysenteriae strains. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 4, p. 331-338, abril 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Concentração inibitória mínima de cepas Brachyspira hyodysenteriae brasileiras. |
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Conteúdo: |
The objectives of this study were to characterize Brachyspira hyodysenteriae isolates and to evaluate the antimicrobial susceptibility patterns of strains obtained from pigs in Brazil based on the minimal inhibitory concentration test (MIC). The MIC was performed for 22 B. hyodysenteriae isolates obtained from 2011 to 2013 using the following antimicrobial drugs: tylosin, tiamulin, valnemulin, doxycycline, lincomycin and tylvalosin. Outbreaks of swine dysentery were diagnosed based on clinical presentation, bacterial isolation, gross and microscopic lesions, duplex PCR for B. hyodysenteriae and B. pilosicoli and nox gene sequencing. All obtained MIC values were consistently higher or equal to the microbiological cut-off described in the literature. The MIC 90 values for the tested drugs were 8?g/ml for doxycycline, >4?g/ml for valnemulin, 8?g/ml for tiamulin, 32?g/ml for tylvalosin, >64?g/ ml for lincomycin and >128?g/ml for tylosin. These results largely corroborate those reported in the literature. Tiamulin, doxycycline and tylvalosin showed the lowest MIC results. All of the samples subjected to phylogenetic analysis based on the nox gene sequence exhibited similar results, showing 100% identity to B. hyodysenteriae. This is the first study describing the MIC pattern of B. hyodysenteriae isolated in Brazil. |
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Palavras-Chave: |
Concentração inibitória mínima; Disenteria suína; Nox gene; Sensibilidade antimicrobiana. |
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Thesagro: |
Diarreia. |
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Thesaurus NAL: |
Brachyspira hyodysenteriae; Diarrhea; Inhibitory concentration 50; Minimum inhibitory concentration; Swine dysentery. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162513/1/Minimum-inhibitory-concentration.pdf
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Marc: |
LEADER 02484naa a2200337 a 4500
001 2073728
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aDANIEL, A. G. S.
245 $aMinimum inhibitory concentration of Brazilian Brachyspira hyodysenteriae strains.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Concentração inibitória mínima de cepas Brachyspira hyodysenteriae brasileiras.
520 $aThe objectives of this study were to characterize Brachyspira hyodysenteriae isolates and to evaluate the antimicrobial susceptibility patterns of strains obtained from pigs in Brazil based on the minimal inhibitory concentration test (MIC). The MIC was performed for 22 B. hyodysenteriae isolates obtained from 2011 to 2013 using the following antimicrobial drugs: tylosin, tiamulin, valnemulin, doxycycline, lincomycin and tylvalosin. Outbreaks of swine dysentery were diagnosed based on clinical presentation, bacterial isolation, gross and microscopic lesions, duplex PCR for B. hyodysenteriae and B. pilosicoli and nox gene sequencing. All obtained MIC values were consistently higher or equal to the microbiological cut-off described in the literature. The MIC 90 values for the tested drugs were 8?g/ml for doxycycline, >4?g/ml for valnemulin, 8?g/ml for tiamulin, 32?g/ml for tylvalosin, >64?g/ ml for lincomycin and >128?g/ml for tylosin. These results largely corroborate those reported in the literature. Tiamulin, doxycycline and tylvalosin showed the lowest MIC results. All of the samples subjected to phylogenetic analysis based on the nox gene sequence exhibited similar results, showing 100% identity to B. hyodysenteriae. This is the first study describing the MIC pattern of B. hyodysenteriae isolated in Brazil.
650 $aBrachyspira hyodysenteriae
650 $aDiarrhea
650 $aInhibitory concentration 50
650 $aMinimum inhibitory concentration
650 $aSwine dysentery
650 $aDiarreia
653 $aConcentração inibitória mínima
653 $aDisenteria suína
653 $aNox gene
653 $aSensibilidade antimicrobiana
700 1 $aSATO, J. P. H.
700 1 $aGABARDO, M. P.
700 1 $aRESENDE, T. P.
700 1 $aBARCELLOS, D. E. S. N. de
700 1 $aPEREIRA, C. E. R.
700 1 $aVANNUCCI, F. A.
700 1 $aGUEDES, R. M. C.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 4, p. 331-338, abril 2017.
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Embrapa Unidades Centrais (AI-SEDE) |
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