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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
15/12/2014 |
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Data da última atualização: |
15/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CUNHA, E. R. da; MARTINS, C. F.; SILVA, G. C.; CUMPA, H. C. B.; BAO, S. N. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC; HEIDI CHRISTINA BESSLER CUMPA, CPAC. |
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Título: |
Effects of prolonged in vitro culture and cryopreservation on viability, DNA Fragmentation, chromosome stability and ultrastructure of bovine cells from amniotic fluid and umbilical cord. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 49, n. 5, p. 806-812, Oct. 2014. |
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ISSN: |
0936-6768 |
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DOI: |
10.1111/rda.12372 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to study cellular types that did not participated in the gastrulation process, amniotic fluid cells (AFCs) and umbilical cord cells (UCCs), in conditions of long-term culture and cryopreserved with different solutions. The AFCs and UCCs were used in a comparative study with ear fibroblast cells (EFCs) that were cultured in vitro until 20 cellular passages and cryopreserved in 10% dimethylsulphoxide (DMSO), 5% dimethyl formamide (DMF) and 7% glycerol (Gly) solutions. The cellular viability, ultrastructure, DNA fragmentation and chromosome stability were evaluated to determine the cellular type most resistant. In all cell types, it was possible to evaluate the AFCs until 15 passages and UCCs until 20 passages with different periods of cellular growth to reach the confluence phase. Solutions containing 10% DMSO ensured viability of 90.33 ± 5.58%, 90.56 ± 4.40% and 81.90 ± 3.31%, respectively for EFCs, AFCs and UCCs, being significantly more efficient and with less variation than other cryoprotectant solutions. The AFCs were more sensitive to cryopreservation and presented low viability rate at the passage 20 (17.2 ± 8.87%). There was no change in karyotype and nuclear fragmentation was low in all cellular passages studied. With the scanning electron analysis was possible the characterization of AFCs and UCCs in suspension. The three cellular types of cells presented different shapes and characteristics on the surface. The results demonstrate that bovine AFCs and UCCs can be isolated, cultured in vitro and cryopreserved in 10% DMSO, not causing damage to DNA and chromosomes. The UCCs were more resistant than AFCs in all aspects. MenosThe objective of this work was to study cellular types that did not participated in the gastrulation process, amniotic fluid cells (AFCs) and umbilical cord cells (UCCs), in conditions of long-term culture and cryopreserved with different solutions. The AFCs and UCCs were used in a comparative study with ear fibroblast cells (EFCs) that were cultured in vitro until 20 cellular passages and cryopreserved in 10% dimethylsulphoxide (DMSO), 5% dimethyl formamide (DMF) and 7% glycerol (Gly) solutions. The cellular viability, ultrastructure, DNA fragmentation and chromosome stability were evaluated to determine the cellular type most resistant. In all cell types, it was possible to evaluate the AFCs until 15 passages and UCCs until 20 passages with different periods of cellular growth to reach the confluence phase. Solutions containing 10% DMSO ensured viability of 90.33 ± 5.58%, 90.56 ± 4.40% and 81.90 ± 3.31%, respectively for EFCs, AFCs and UCCs, being significantly more efficient and with less variation than other cryoprotectant solutions. The AFCs were more sensitive to cryopreservation and presented low viability rate at the passage 20 (17.2 ± 8.87%). There was no change in karyotype and nuclear fragmentation was low in all cellular passages studied. With the scanning electron analysis was possible the characterization of AFCs and UCCs in suspension. The three cellular types of cells presented different shapes and characteristics on the surface. The results demonstrate that ... Mostrar Tudo |
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Palavras-Chave: |
Cordão umbilical; Líquido aminiótico. |
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Thesagro: |
Bovino; Criopreservação; Melhoramento genético animal. |
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Thesaurus Nal: |
Amniotic fluid; Cattle; Cryopreservation; Genetic improvement; Umbilical cord. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02681naa a2200313 a 4500
001 2002603
005 2014-12-15
008 2014 bl uuuu u00u1 u #d
022 $a0936-6768
024 7 $a10.1111/rda.12372$2DOI
100 1 $aCUNHA, E. R. da
245 $aEffects of prolonged in vitro culture and cryopreservation on viability, DNA Fragmentation, chromosome stability and ultrastructure of bovine cells from amniotic fluid and umbilical cord.
260 $c2014
520 $aThe objective of this work was to study cellular types that did not participated in the gastrulation process, amniotic fluid cells (AFCs) and umbilical cord cells (UCCs), in conditions of long-term culture and cryopreserved with different solutions. The AFCs and UCCs were used in a comparative study with ear fibroblast cells (EFCs) that were cultured in vitro until 20 cellular passages and cryopreserved in 10% dimethylsulphoxide (DMSO), 5% dimethyl formamide (DMF) and 7% glycerol (Gly) solutions. The cellular viability, ultrastructure, DNA fragmentation and chromosome stability were evaluated to determine the cellular type most resistant. In all cell types, it was possible to evaluate the AFCs until 15 passages and UCCs until 20 passages with different periods of cellular growth to reach the confluence phase. Solutions containing 10% DMSO ensured viability of 90.33 ± 5.58%, 90.56 ± 4.40% and 81.90 ± 3.31%, respectively for EFCs, AFCs and UCCs, being significantly more efficient and with less variation than other cryoprotectant solutions. The AFCs were more sensitive to cryopreservation and presented low viability rate at the passage 20 (17.2 ± 8.87%). There was no change in karyotype and nuclear fragmentation was low in all cellular passages studied. With the scanning electron analysis was possible the characterization of AFCs and UCCs in suspension. The three cellular types of cells presented different shapes and characteristics on the surface. The results demonstrate that bovine AFCs and UCCs can be isolated, cultured in vitro and cryopreserved in 10% DMSO, not causing damage to DNA and chromosomes. The UCCs were more resistant than AFCs in all aspects.
650 $aAmniotic fluid
650 $aCattle
650 $aCryopreservation
650 $aGenetic improvement
650 $aUmbilical cord
650 $aBovino
650 $aCriopreservação
650 $aMelhoramento genético animal
653 $aCordão umbilical
653 $aLíquido aminiótico
700 1 $aMARTINS, C. F.
700 1 $aSILVA, G. C.
700 1 $aCUMPA, H. C. B.
700 1 $aBAO, S. N.
773 $tReproduction in Domestic Animals$gv. 49, n. 5, p. 806-812, Oct. 2014.
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Embrapa Cerrados (CPAC) |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
25/09/2013 |
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Data da última atualização: |
26/09/2018 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
TEIXEIRA, F. F.; MIRANDA, R. A. de; PAES, M. C. D.; SOUSA, S. M. de; GAMA, E. E. G. e. |
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Afiliação: |
FLAVIA FRANCA TEIXEIRA, CNPMS; RUBENS AUGUSTO DE MIRANDA, CNPMS; MARIA CRISTINA DIAS PAES, CNPMS; SYLVIA MORAIS DE SOUSA TINOCO, CNPMS; ELTO EUGÊNIO GOMES E GAMA. |
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Título: |
Melhoramento do milho-doce. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Sete Lagoas: Embrapa Milho e Sorgo, 2013. |
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Páginas: |
32 p. |
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Descrição Física: |
il. |
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Série: |
(Embrapa Milho e Sorgo. Documentos, 154). |
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Idioma: |
Português |
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Conteúdo: |
O milho-doce é considerado um milho especial pois tem a concentração de açucares no grão elevadas quando comparado ao milho comum. Essas variações são controladas geneticamente, o que faz com que os programas de melhoramento de milho-doce sejam conduzidos em separado dos programas de melhoramento do milho comum. Nesses programas, além da elevado teor do açucar no grão, são considerados critérios agronômico e de qualidade. O objetivo desse comunicado técnico é apresentar uma revisão sobre aspectos relevantes no melhoramento do milho-doce, tais como a importância econômica, mutantes de milho-doce utilizados, recursos genéticos e melhoramento e o programa de milho-doce na Embrapa. |
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Palavras-Chave: |
Melhoramento genético. |
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Thesagro: |
Melhoramento vegetal; Milho; Variedade. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/90123/1/doc-154.pdf
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Marc: |
LEADER 01340nam a2200229 a 4500
001 1967082
005 2018-09-26
008 2013 bl uuuu u0uu1 u #d
100 1 $aTEIXEIRA, F. F.
245 $aMelhoramento do milho-doce.$h[electronic resource]
260 $aSete Lagoas: Embrapa Milho e Sorgo$c2013
300 $a32 p.$cil.
490 $a(Embrapa Milho e Sorgo. Documentos, 154).
520 $aO milho-doce é considerado um milho especial pois tem a concentração de açucares no grão elevadas quando comparado ao milho comum. Essas variações são controladas geneticamente, o que faz com que os programas de melhoramento de milho-doce sejam conduzidos em separado dos programas de melhoramento do milho comum. Nesses programas, além da elevado teor do açucar no grão, são considerados critérios agronômico e de qualidade. O objetivo desse comunicado técnico é apresentar uma revisão sobre aspectos relevantes no melhoramento do milho-doce, tais como a importância econômica, mutantes de milho-doce utilizados, recursos genéticos e melhoramento e o programa de milho-doce na Embrapa.
650 $aMelhoramento vegetal
650 $aMilho
650 $aVariedade
653 $aMelhoramento genético
700 1 $aMIRANDA, R. A. de
700 1 $aPAES, M. C. D.
700 1 $aSOUSA, S. M. de
700 1 $aGAMA, E. E. G. e
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Embrapa Milho e Sorgo (CNPMS) |
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