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| Registros recuperados : 150 | |
| 4. |  | OLIVARES, C.; FONSECA, J. F. da; SARAIVA, H.; ALFRADIQUE, V.; CÔRTES, L.; COSTA, L.; BRANDÃO, F.; SOUZA-FABJAN, J. M. Influence of Mini-Percoll techniques on motility parameters of ram frozen-thawed sperm. In: INTERNATIONAL CONGRESS OF ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tous: Society for Reprocutive Biololy, 2016. p. 168-169. Abstract PW171 Poster.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | FONSECA, J. F. da; MACHADO, V. de O.; PAIVA, M. P. S. L. M. de; FACO, O.; SOUZA-FABJAN, J. M. G. Recent advances in goat artificial insemination in Brazil. Revista Brasileira de Reprodução Animal, v. 43, n. 2, p. 66-71, abr./jun. 2019. Edição dos anais do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | FONSECA, J. F. da; ALVIM, G. P.; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; BRAIR, V. L.; BRANDÃO, F. Z.; FACO, O. Reproductive features and use of an anti-inflammatory drug in estrus-induced dairy goats artificially inseminated in a standing position with cervix immobilization. Reproductive Biology, Olsztyn, v. 17, n. 3, p. 268-273, Sep. 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 10. | | MAIA, A. L. R. S.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; BALARO, M. F. A.; OLIVEIRA, M. E. F.; FACO, O.; FONSECA, J. F. da. Reproductive parameters of dairy goats after receiving two doses of d-cloprostenol at different intervals. Animal Reproduction Science, v. 1981, p. 16-23, June, 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | SOUZA-FABJAN, J. M. G.; TORRES, C. A. A.; MAIA, A. L. R. S.; BRANDÃO, F. Z.; OBA, E.; BELTOLDO, M. J.; FONSECA, J. F. da. Re-used progesterone devices efficiently synchronise oestrus and ovulation after autoclaving process in Toggenburg goats during the breeding season. Animal Production Science, Victoria, v. 55, n. 6, p. 818-822, 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 12. | | SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; GUIMARÃES, M. P. P.; BRANDÃO, F. Z.; BARTLEWSK, P. M.; FONSECA, J. F. da. Review: Non-surgical artificial insemination and embryo recovery as safe tools for genetic preservation in small ruminants. Animal. The International Journal of Animal Biosciences, v. 17, e100787, May, 2023. Supplement 1. Review.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 14. | | BRAIR, V. L.; ANDRADE, A. B. P.; MORAIS, M. C. C.; RANGEL, P. S. C.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Short-term hormonal protocol efficiency either with or without equine chorionic gonadotrophin to promote oestrous synchronization in cyclic dairy goats. Reproduction, Fertility and Development, v. 33, n. 1/2, p. 165, 2021. Edição dos Proceedings of the 47th Annual Conference of the International Embryo Technology (IETS) Virtual Meeting, January 18-21, 2021.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | MORAIS, M. C. C.; ARRAIS, A. M.; SILVA, N. C.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Association of controlled light program, cloprostenol and gonadotropins for synchronous estrus induction and fixed time artificial insemination in dairy goats during the non-breeding season: preliminary results. Animal Reproduction, v. 17, n. 3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online).| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | PINTO, P. H. N.; FREITAS, J. A.; FONSECA, J. D.; PILE, E. A.; ESTEVES, L. V.; SOUZA, V. L.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Artificial insemination in Cape Verdean Goats with cooled semen stored for 24 or 48 Hours. Australian Journal of Basic and Applied Sciences, v. 8, n. 18, p. 473-478, Dec. 2014.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; SOUZA FABJAN, J. M. G.; PRATES, J. F.; TREVIZAN, J. T.; BRANDÃO, F. Z.; FONSECA, J. F. da. Goat incubator: the doe as a life incubator of bovine oocytes - first step. Animal Reprodroduction, v. 14, n. 3, p. 738, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | MAIA, A. L. R. S.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; ARAÚJO, M. C. C.; SIQUEIRA, L. G. B.; FACO, O.; FONSECA, J. F. da. Hydrosalpinx in dairy goats: occurrence, ultrasound diagnosis, macro- and microscopic characterization. Small Ruminant Research, v. 160, p. 5-11, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| Registros recuperados : 150 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. |
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Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; Ribrio Ivan Tavares Pereira Batista; Juliane Teramachi Trevizan; Joanna Maria Gonçalves Souza-Fabjan; Felipe Zandonadi Brandão; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019. |
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DOI: |
10.5433/1679-0359.2019v40n6Supl3p3789 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation. MenosAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that trans... Mostrar Tudo |
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Palavras-Chave: |
Bovine oocytes; COCs; Ex-situ maturation; In vitro maturation; Nonsurgical embryo transfer technique. |
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Thesaurus NAL: |
Goats. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208736/1/Goat-incubator-can-bovine-oocytes.pdf
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Marc: |
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024 7 $a10.5433/1679-0359.2019v40n6Supl3p3789$2DOI
100 1 $aFONSECA, J. F. da
245 $aGoat incubator$bcan bovine oocytes be matured in the uterine horn of a goat?$h[electronic resource]
260 $c2019
520 $aAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation.
650 $aGoats
653 $aBovine oocytes
653 $aCOCs
653 $aEx-situ maturation
653 $aIn vitro maturation
653 $aNonsurgical embryo transfer technique
700 1 $aBATISTA, R. I. T. P.
700 1 $aTREVIZAN, J. T.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aBRANDÃO, F. Z.
700 1 $aCAMARGO, L. S. de A.
773 $tSemina: Ciências Agrárias, Londrina$gv. 40, n. 6, supp. 3, p. 3789-3796, 2019.
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Embrapa Gado de Leite (CNPGL) |
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