02452naa a2200265 a 450000100080000000500110000800800410001902400470006010000220010724501040012926000090023352016150024265000100185765300190186765300090188665300230189565300240191865300420194270000250198470000200200970000270202970000200205670000250207677300850210121187092024-02-06       2019    bl uuuu     u00u1 u    #d7 a10.5433/1679-0359.2019v40n6Supl3p37892DOI1 aFONSECA, J. F. da  aGoat incubatorbcan bovine oocytes be matured in the uterine horn of a goat?h[electronic resource]  c2019  aAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p &lt 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation.  aGoats  aBovine oocytes  aCOCs  aEx-situ maturation  aIn vitro maturation  aNonsurgical embryo transfer technique1 aBATISTA, R. I. T. P.1 aTREVIZAN, J. T.1 aSOUZA-FABJAN, J. M. G.1 aBRANDÃO, F. Z.1 aCAMARGO, L. S. de A.  tSemina: Ciências Agrárias, Londrinagv. 40, n. 6, supp. 3, p. 3789-3796, 2019.