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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
18/09/2020 |
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Data da última atualização: |
17/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MELO, B. P. de; LOURENCO-TESSUTTI, I. T.; MORGANTE, C. V.; SANTOS, N. C.; PINHEIRO, L. B.; LINS, C. B. de J.; SILVA, M. C. M.; MACEDO, L. L. P.; FONTES, E. P. B.; GROSSI-DE-SA, M. F. |
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Afiliação: |
BRUNO PAES DE MELO, UFV; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; NAIARA CORDEIRO SANTOS; LUANNA BEZERRA PINHEIRO, UCB; CAMILA BARROZO DE JESUS LINS; MARIA CRISTINA MATTAR DA SILVA, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ELIZABETH PACHECO BATISTA FONTES, UFV; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Soybean embryonic axis transformation: combining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 11, article 1228, 2020. |
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DOI: |
https://doi.org/10.3389/fpls.2020.01228 |
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Idioma: |
Inglês |
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Conteúdo: |
The first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs. MenosThe first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for stud... Mostrar Tudo |
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Palavras-Chave: |
Agrobacterium-mediated transformation; Embryonic axis; High-efficiency plant transformation; Particle bombardment; Planta geneticamente modificada; Recuperação trangênica de soja. |
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Thesagro: |
Cultura de Tecido; Glycine Max; Soja. |
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Thesaurus Nal: |
Agrobacterium; Embryonic structures; Genetic transformation; Plant genetics. |
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Categoria do assunto: |
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G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216085/1/fpls-11-01228.pdf
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Marc: |
LEADER 02941naa a2200397 a 4500
001 2125013
005 2020-11-17
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fpls.2020.01228$2DOI
100 1 $aMELO, B. P. de
245 $aSoybean embryonic axis transformation$bcombining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration.$h[electronic resource]
260 $c2020
520 $aThe first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs.
650 $aAgrobacterium
650 $aEmbryonic structures
650 $aGenetic transformation
650 $aPlant genetics
650 $aCultura de Tecido
650 $aGlycine Max
650 $aSoja
653 $aAgrobacterium-mediated transformation
653 $aEmbryonic axis
653 $aHigh-efficiency plant transformation
653 $aParticle bombardment
653 $aPlanta geneticamente modificada
653 $aRecuperação trangênica de soja
700 1 $aLOURENCO-TESSUTTI, I. T.
700 1 $aMORGANTE, C. V.
700 1 $aSANTOS, N. C.
700 1 $aPINHEIRO, L. B.
700 1 $aLINS, C. B. de J.
700 1 $aSILVA, M. C. M.
700 1 $aMACEDO, L. L. P.
700 1 $aFONTES, E. P. B.
700 1 $aGROSSI-DE-SA, M. F.
773 $tFrontiers in Plant Science$gv. 11, article 1228, 2020.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/12/2016 |
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Data da última atualização: |
10/02/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SAMPAIO, A. H. R.; COELHO FILHO, M. A.; SOUZA, L. D.; BRITO, R. B. F.; SILVA, R. O. da. |
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Afiliação: |
ANTONIO HÉLDER RODRIGUES SAMPAIO, Federal Institute of Science; MAURICIO ANTONIO COELHO FILHO, CNPMF; LAERCIO DUARTE SOUZA, CNPMF; RALPH BRUNO FRANÇA BRITO, UFRB; ROBERVAL OLIVEIRA DA SILVA, UFRB. |
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Título: |
Yield and quality of 'pera' sweet orange grafted on different rootstocks under rainfed conditions. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Revista Brasileira Fruticultura, v. 38, n. 3, Jaboticabal,SP, May/ June, 2016. |
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ISSN: |
0100-2945 |
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DOI: |
1590/0100-29452016770 |
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Idioma: |
Italiano |
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Conteúdo: |
This study aimed to evaluate, under field conditions, different combinations between 'Pera' sweet orange and eight rootstocks: 'Rangpur' lime (RL), 'Volkamer' lemon (VL), 'Cleopatra' mandarin (CM), 'Sunki Maravilha' mandarin (SMM), 'Indio and''Riverside' citrandarins, and VL x RL (?Rangpur? lime)-010 and TH-051 hybrids. The soil water matric potential (?m) was characterized for all scion-rootstock combinations at distance of 1.0m from the trunk at the plant row direction and depths of 0.25 m, 0.50 m 0.90 m in the dry and wet seasons. For two years, fruit production parameters and fruit quality were assessed. Differences of Ym among scion-rootstock combinations were observed during the dry season (p?0.05). The lowest Ym values for RL and the highest for TH-051 indicate the existence of different intrinsic mechanisms affecting the water extraction of each scion-rootstock combination. Rootstocks have influenced fruit yield and quality (p?0.05). The best combinations for fruit quality and production were sweet orange grafted on 'Riverside', 'Indio' and TH-051 rootstocks. |
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Thesagro: |
Irrigação; Laranja. |
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Thesaurus NAL: |
Irrigation; Oranges. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 01811naa a2200241 a 4500
001 2058455
005 2017-02-10
008 2016 bl uuuu u00u1 u #d
022 $a0100-2945
024 7 $a1590/0100-29452016770$2DOI
100 1 $aSAMPAIO, A. H. R.
245 $aYield and quality of 'pera' sweet orange grafted on different rootstocks under rainfed conditions.$h[electronic resource]
260 $c2016
520 $aThis study aimed to evaluate, under field conditions, different combinations between 'Pera' sweet orange and eight rootstocks: 'Rangpur' lime (RL), 'Volkamer' lemon (VL), 'Cleopatra' mandarin (CM), 'Sunki Maravilha' mandarin (SMM), 'Indio and''Riverside' citrandarins, and VL x RL (?Rangpur? lime)-010 and TH-051 hybrids. The soil water matric potential (?m) was characterized for all scion-rootstock combinations at distance of 1.0m from the trunk at the plant row direction and depths of 0.25 m, 0.50 m 0.90 m in the dry and wet seasons. For two years, fruit production parameters and fruit quality were assessed. Differences of Ym among scion-rootstock combinations were observed during the dry season (p?0.05). The lowest Ym values for RL and the highest for TH-051 indicate the existence of different intrinsic mechanisms affecting the water extraction of each scion-rootstock combination. Rootstocks have influenced fruit yield and quality (p?0.05). The best combinations for fruit quality and production were sweet orange grafted on 'Riverside', 'Indio' and TH-051 rootstocks.
650 $aIrrigation
650 $aOranges
650 $aIrrigação
650 $aLaranja
700 1 $aCOELHO FILHO, M. A.
700 1 $aSOUZA, L. D.
700 1 $aBRITO, R. B. F.
700 1 $aSILVA, R. O. da
773 $tRevista Brasileira Fruticultura$gv. 38, n. 3, Jaboticabal,SP, May/ June, 2016.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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