02941naa a2200397 a 450000100080000000500110000800800410001902400490006010000190010924501890012826000090031752016260032665000180195265000250197065000270199565000190202265000220204165000160206365000090207965300420208865300190213065300410214965300250219065300360221565300380225170000290228970000200231870000180233870000200235670000220237670000200239870000210241870000210243970000240246077300590248421250132020-11-17       2020    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.3389/fpls.2020.012282DOI1 aMELO, B. P. de  aSoybean embryonic axis transformationbcombining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration.h[electronic resource]  c2020  aThe first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs.  aAgrobacterium  aEmbryonic structures  aGenetic transformation  aPlant genetics  aCultura de Tecido  aGlycine Max  aSoja  aAgrobacterium-mediated transformation  aEmbryonic axis  aHigh-efficiency plant transformation  aParticle bombardment  aPlanta geneticamente modificada  aRecuperação trangênica de soja1 aLOURENCO-TESSUTTI, I. T.1 aMORGANTE, C. V.1 aSANTOS, N. C.1 aPINHEIRO, L. B.1 aLINS, C. B. de J.1 aSILVA, M. C. M.1 aMACEDO, L. L. P.1 aFONTES, E. P. B.1 aGROSSI-DE-SA, M. F.  tFrontiers in Plant Sciencegv. 11, article 1228, 2020.