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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
30/11/2021 |
Data da última atualização: |
30/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GUERRA, P. A.; SOUZA, E. H. de; ANDRADE, E. C. de; MAX, D. de A. S.; OLIVEIRA, R. S.; SOUZA, F. V. D. |
Afiliação: |
PATRÍCIA ARAÚJO GUERRA, UFRB; EVERTON HILO DE SOUZA, UFRB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; DANIELA DE ANDRADE SILVA MAX, UFRB; RAFAELLE SOUZA DE OLIVEIRA, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
Título: |
Comparison of shoot tip culture and cryotherapy for eradication of ampeloviruses associated with Pineapple mealybug wilt in wild varieties |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology - Plant, 2020. |
ISSN: |
1475-2689 |
Idioma: |
Inglês |
Conteúdo: |
The Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed with virus-free plants. MenosThe Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed wi... Mostrar Tudo |
Thesagro: |
Abacaxi; Planta Ornamental. |
Categoria do assunto: |
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Marc: |
LEADER 02208naa a2200217 a 4500 001 2136827 005 2021-11-30 008 2020 bl uuuu u00u1 u #d 022 $a1475-2689 100 1 $aGUERRA, P. A. 245 $aComparison of shoot tip culture and cryotherapy for eradication of ampeloviruses associated with Pineapple mealybug wilt in wild varieties$h[electronic resource] 260 $c2020 520 $aThe Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed with virus-free plants. 650 $aAbacaxi 650 $aPlanta Ornamental 700 1 $aSOUZA, E. H. de 700 1 $aANDRADE, E. C. de 700 1 $aMAX, D. de A. S. 700 1 $aOLIVEIRA, R. S. 700 1 $aSOUZA, F. V. D. 773 $tIn Vitro Cellular & Developmental Biology - Plant, 2020.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
27/12/2017 |
Data da última atualização: |
01/02/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ASSIS, S. L. F. de; REIS, R. C.; VIANA, E. de S.; ALMEIDA, J. M.; SANTOS, B. de J. R. dos. |
Afiliação: |
STEPHANIE LIMA FERREIRA DE ASSIS, Faculdade Maria Milza; RONIELLI CARDOSO REIS, CNPMF; ELISETH DE SOUZA VIANA, CNPMF; JAMILLE MOTA ALMEIDA, Faculdade Maria Milza; BEATRIZ DE JESUS REZENDE DOS SANTOS, Faculdade Maria Milza. |
Título: |
Determinação dos perfis de viscosidade de amidos extraídos de bananas e plátanos |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 17., 2017 Ciência e Empreendedorismo : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2017. 137p. |
Páginas: |
1p. |
Idioma: |
Português |
Notas: |
Novos usos de mandiocas e fruteiras |
Thesagro: |
Banana. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169929/1/NUMF-D3-094-17-V01-Aprovado.pdf
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Marc: |
LEADER 00700nam a2200181 a 4500 001 2083721 005 2018-02-01 008 2017 bl uuuu u00u1 u #d 100 1 $aASSIS, S. L. F. de 245 $aDeterminação dos perfis de viscosidade de amidos extraídos de bananas e plátanos$h[electronic resource] 260 $aIn: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 17., 2017 Ciência e Empreendedorismo : resumos. Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2017. 137p.$c2017 300 $a1p. 500 $aNovos usos de mandiocas e fruteiras 650 $aBanana 700 1 $aREIS, R. C. 700 1 $aVIANA, E. de S. 700 1 $aALMEIDA, J. M. 700 1 $aSANTOS, B. de J. R. dos
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