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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
29/11/2023 |
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Autoria: |
IRITANI, A.; NISHIKAWA, Y.; NAGASAWA, S. |
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Título: |
Studies on the egg-yolk coagulating enzyme in goat semen. VII. Variations in the enzyme activity of the semen between breeding season and non-breeding season, and in each ejaculate collected three times successively. |
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Ano de publicação: |
1964 |
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Fonte/Imprenta: |
Japanese Journal of Animal Reproduction, v. 10, n. 2, p. 52-56, 1964. |
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DOI: |
https://doi.org/10.1262/jrd1955.10.52 |
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Idioma: |
Japonês |
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Conteúdo: |
Some of the factors affecting the activity of the egg-yolk coagulating enzyme in goat semen have been reported in the previous report of this study. This experiment was performed to clarify the variations in the enzyme activity owing to the collection time and the season when the semen was collected throughout the year, March 1960 to April 1962. Besides, some seminal constituents, fructose, citric acid and protein, that are probably secreted from the seminal vesicle were determined quantitatively in comparison with the activity of the egg-yolk coagulating enzyme which may be secreted from the Cowper's gland. And thus we intended to examine the presence of any relationship between the seminal vesicle constituents and the Cowper's gland secretion. Principal results obtained are as follows. Activity of the egg-yolk coagulating enzyme was significantly higher in the breeding season, August to March, than in the non-breeding season in each goat. 2. It was recognized that the enzyme activity of the seminal plasma was enhanced significantly as the collection time was advanced, from the results of averaging 110 trials of three successive ejaculations on three goats throughout the year. 3. Concentrations of fructose, citric acid and protein in seminal plasma were significantly higher in the breeding season than in the non-breeding season as in the case of the coagulating enzyme, but there was no significant difference among the collection time, 1 st, 2 nd and the 3 rd in contrast to the results obtained in the enzyme activity. So it was suggested that some difference might be seen in the secreting mechanism between the seminal vesicle and the Cowper's gland MenosSome of the factors affecting the activity of the egg-yolk coagulating enzyme in goat semen have been reported in the previous report of this study. This experiment was performed to clarify the variations in the enzyme activity owing to the collection time and the season when the semen was collected throughout the year, March 1960 to April 1962. Besides, some seminal constituents, fructose, citric acid and protein, that are probably secreted from the seminal vesicle were determined quantitatively in comparison with the activity of the egg-yolk coagulating enzyme which may be secreted from the Cowper's gland. And thus we intended to examine the presence of any relationship between the seminal vesicle constituents and the Cowper's gland secretion. Principal results obtained are as follows. Activity of the egg-yolk coagulating enzyme was significantly higher in the breeding season, August to March, than in the non-breeding season in each goat. 2. It was recognized that the enzyme activity of the seminal plasma was enhanced significantly as the collection time was advanced, from the results of averaging 110 trials of three successive ejaculations on three goats throughout the year. 3. Concentrations of fructose, citric acid and protein in seminal plasma were significantly higher in the breeding season than in the non-breeding season as in the case of the coagulating enzyme, but there was no significant difference among the collection time, 1 st, 2 nd and the 3 rd in contrast to ... Mostrar Tudo |
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Palavras-Chave: |
Semen collection. |
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Thesagro: |
Bioquímica; Caprino; Enzima; Reprodução; Sêmen. |
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Thesaurus Nal: |
Animal reproduction; Biochemistry; Enzyme activity; Goats. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02580naa a2200277 a 4500
001 1523735
005 2023-11-29
008 1964 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1262/jrd1955.10.52$2DOI
100 1 $aIRITANI, A.
245 $aStudies on the egg-yolk coagulating enzyme in goat semen. VII. Variations in the enzyme activity of the semen between breeding season and non-breeding season, and in each ejaculate collected three times successively.
260 $c1964
520 $aSome of the factors affecting the activity of the egg-yolk coagulating enzyme in goat semen have been reported in the previous report of this study. This experiment was performed to clarify the variations in the enzyme activity owing to the collection time and the season when the semen was collected throughout the year, March 1960 to April 1962. Besides, some seminal constituents, fructose, citric acid and protein, that are probably secreted from the seminal vesicle were determined quantitatively in comparison with the activity of the egg-yolk coagulating enzyme which may be secreted from the Cowper's gland. And thus we intended to examine the presence of any relationship between the seminal vesicle constituents and the Cowper's gland secretion. Principal results obtained are as follows. Activity of the egg-yolk coagulating enzyme was significantly higher in the breeding season, August to March, than in the non-breeding season in each goat. 2. It was recognized that the enzyme activity of the seminal plasma was enhanced significantly as the collection time was advanced, from the results of averaging 110 trials of three successive ejaculations on three goats throughout the year. 3. Concentrations of fructose, citric acid and protein in seminal plasma were significantly higher in the breeding season than in the non-breeding season as in the case of the coagulating enzyme, but there was no significant difference among the collection time, 1 st, 2 nd and the 3 rd in contrast to the results obtained in the enzyme activity. So it was suggested that some difference might be seen in the secreting mechanism between the seminal vesicle and the Cowper's gland
650 $aAnimal reproduction
650 $aBiochemistry
650 $aEnzyme activity
650 $aGoats
650 $aBioquímica
650 $aCaprino
650 $aEnzima
650 $aReprodução
650 $aSêmen
653 $aSemen collection
700 1 $aNISHIKAWA, Y.
700 1 $aNAGASAWA, S.
773 $tJapanese Journal of Animal Reproduction$gv. 10, n. 2, p. 52-56, 1964.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
18/11/2020 |
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Data da última atualização: |
18/11/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SANTOS, F. C.; ROSA, S. D. V. F. da; GROTEWOLD, E.; PADILHA, L. |
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Afiliação: |
The Ohio State University; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; The Ohio State University; LILIAN PADILHA, CNPCa. |
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Título: |
Efficiency of coffee seeds rna extraction protoco using rna integrity number analysis. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia. Leveraging knowledge for coffee sustainability: proceedings. Armenia: Association for Science and Information on Coffee, 2014. p. 273-274. |
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Idioma: |
Português |
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Conteúdo: |
Coffee is one of the most important agricultural products in the world market. The mode of coffee processing, whether wet or dry, determines the flavor characteristic and also the seed quality. Quality RNA isolation is a mandatory requirement for studies of gene expression, including reverse transcriptase (RT), real-time quantitative PCR (RT-qPCR), construction of cDNA libraries, or microarray analyses. Due to the presence of secondary metabolites, polysaccharides, and polyphenols, standardization of a quality RNA extraction for different coffee plant tissue is very difficult. Also, getting high-quality RNA may be complicated because of RNA susceptibility to RNase degradation. |
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Thesagro: |
Café; Extração; RNA; Semente. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/217961/1/PB240.pdf
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Marc: |
LEADER 01397nam a2200193 a 4500
001 2126783
005 2020-11-18
008 2014 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. C.
245 $aEfficiency of coffee seeds rna extraction protoco using rna integrity number analysis.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia. Leveraging knowledge for coffee sustainability: proceedings. Armenia: Association for Science and Information on Coffee, 2014. p. 273-274.$c2014
520 $aCoffee is one of the most important agricultural products in the world market. The mode of coffee processing, whether wet or dry, determines the flavor characteristic and also the seed quality. Quality RNA isolation is a mandatory requirement for studies of gene expression, including reverse transcriptase (RT), real-time quantitative PCR (RT-qPCR), construction of cDNA libraries, or microarray analyses. Due to the presence of secondary metabolites, polysaccharides, and polyphenols, standardization of a quality RNA extraction for different coffee plant tissue is very difficult. Also, getting high-quality RNA may be complicated because of RNA susceptibility to RNase degradation.
650 $aCafé
650 $aExtração
650 $aRNA
650 $aSemente
700 1 $aROSA, S. D. V. F. da
700 1 $aGROTEWOLD, E.
700 1 $aPADILHA, L.
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Registro original: |
Embrapa Café (CNPCa) |
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