01397nam a2200193 a 450000100080000000500110000800800410001910000180006024501140007826002170019252006900040965000100109965000150110965000080112465000120113270000250114470000180116970000160118721267832020-11-18       2014    bl uuuu     u00u1 u    #d1 aSANTOS, F. C.  aEfficiency of coffee seeds rna extraction protoco using rna integrity number analysis.h[electronic resource]  aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia. Leveraging knowledge for coffee sustainability: proceedings. Armenia: Association for Science and Information on Coffee, 2014. p. 273-274.c2014  aCoffee is one of the most important agricultural products in the world market. The mode of coffee processing, whether wet or dry, determines the flavor characteristic and also the seed quality. Quality RNA isolation is a mandatory requirement for studies of gene expression, including reverse transcriptase (RT), real-time quantitative PCR (RT-qPCR), construction of cDNA libraries, or microarray analyses. Due to the presence of secondary metabolites, polysaccharides, and polyphenols, standardization of a quality RNA extraction for different coffee plant tissue is very difficult. Also, getting high-quality RNA may be complicated because of RNA susceptibility to RNase degradation.  aCafé  aExtração  aRNA  aSemente1 aROSA, S. D. V. F. da1 aGROTEWOLD, E.1 aPADILHA, L.