| |
|
|
| Registros recuperados : 13 | |
| 3. |  | BIER, D.; TUTIJA, J. F.; PASQUATTI, T. N.; OLIVEIRA, T. L.; ARAUJO, F. R.; VERBISCK, N. V. Identificação por espectrometria de massa MALDI-TOF de Salmonella spp. e Escherichia coli isolados de carcaças bovinas. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 12, p. 1373-1379, dezembro 2017. Título em inglês: MALDI-TOF mass spectrometry identification of Salmonella spp. and Escherichia coli isolated from bovine carcasses.| Biblioteca(s): Embrapa Gado de Corte; Embrapa Unidades Centrais. |
|    |
| 4. | | RAMOS, I. N. de A.; OVELAR, L. F. V.; MACHADO, M. de A.; ARECO, M. S.; PASQUATTI, T. N.; ARAUJO, F. R.; BIER, D.; RODRIGUES, R. de A. Isolation of staphylococcus aureus, Salmonella spp. and Verotoxigenic Escherichia colin in bovine meats marketed in supermarkets and free fairs in Campo Grande - Mato Grosso do Sul. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 30., 2019, Maceió. Resumos... São Paulo, SP: Sociedade Brasileira de Microbiologia -SBM, 2019.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 5. | | BACANELLI, G.; MONTOVANI, C.; LOUZAN, A. L.; PASQUATTI, T.; BIER, D.; ROSINHA, G. M. S.; ARAUJO, F. R.; VERBISCK, N. V. MALDI-TOF mass spectrometry identification and detection of relevant pathogens in beef cattle. In: INTERNATIONAL MASS SPETROMETRY CONFERENCE, 22., 2018. Abstract book... Florence, Italy, 2018. p. 189-190 Oral communications.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 6. | | ARECO, M. S.; RAMOS, I. N. de A.; OVELAR, L. F. V.; MACHADO, M. de A.; PASQUATTI, T. N.; ARAUJO, F. R.; BIER, D. Sensitivity profile of isolated potential bacteria of bovine meats marketed in supermarkets and free fairs in Campo Grande - Mato Grosso do Sul. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 30., 2019, Maceió. Resumos... São Paulo, SP: Sociedade Brasileira de Microbiologia -SBM, 2019.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 7. | | CARNEIRO, P. A. M.; TAKATANI, H.; PASQUATTI, T. N.; SILVA, C. B. D. G.; NORBY, B.; WILKINS, M. J.; ZUMÁRRAGA, M. J.; ARAUJO, F. R.; KANEENE, J. B. Epidemiological Study of Mycobacterium bovis Infection in Buffalo and Cattle in Amazonas, Brazil. Frontiers in Veterinary Science, v. 6, article 434, December, 2019.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 8. | | CARNEIRO, P. A. M.; PASQUATTI, T. N.; TAKANI, H.; ZUMÁRRAGA, M. J.; MARFIL, M. J.; BARNARD, C.; FITZGERALD, S. D.; ABRAMOVITCH, R. B.; ARAUJO, F. R.; KANEENE, J. B. Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil. Veterinary Medicine and Science, v. 6, p. 133?141, 2020.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 9. | | CARNEIRO, P. A. M.; PASQUATTI, T. N.; TAKATANI, H.; ZUMÁRRAGA, M. J.; MARFIL, M. J.; BARNARD, C.; FITZGERALD, S. D.; ABRAMOVITCH, R. B.; ARAUJO, F. R.; KANEEENE, J. B. Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil. Veterinary Medicine and Science, v. 6, n. 1, p. 1-9, December, 2019.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 10. | | CARNEIRO, P. A. M.; PASQUATTI, T. N.; LIMA, D. A. R.; RODRIGUES, R. A.; TAKANI, H.; SILVA, C. B. D. G.; JARDIM, R.; ABRAMOVITCH, R. B.; WILKINS, M. J.; DAVILA, A. M. R.; ARAUJO, F. R.; KANEENE, J. B. Milk contamination by Mycobacterium tuberculosis complex, implications for public health in Amazonas, Brazil. Journal of Food Protection, 2022.| Biblioteca(s): Embrapa Gado de Corte. |
|  |
| 11. | | BACANELLI, G. M.; OLARTE, L. C.; LEGUIZAMON, G. O. de C.; SILVA, M. R.; RODIGUES, R. A.; PASQUATTI, T. N.; CARNEIRO, P. A.; ETGES, R. N.; ARAUJO, F. R.; VERBISCK, N. V. Diagnóstico de tuberculose bovina: avanços na identificação de Mycobacterium bovis por espectrometria de massas MALDI-TOF. Campo Grande, MS: Embrapa Gado de Corte, 2020. (Embrapa Gado de Corte. Comunicado Técnico, 146)| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 12. | | BACANELLI, G.; OLARTE, L. C.; SILVA, M. R.; RODRIGUES, R. A.; CARNEIRO, P. A. M.; KANEENE, J. B.; PASQUATTI, T. N.; TAKATANI, H.; ZUMÁRRAGA, M. J.; ETGES, R. N.; ARAUJO, F. R.; VERBISCK, N. V. Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae. Journal the Veterinary Medical Science, v. 81, n. 10, p.1400?1408, 2019.| Biblioteca(s): Embrapa Gado de Corte; Embrapa Gado de Leite. |
| |
| 13. | | CARNEIRO, P. A.; ZIMPEL, C. K.; PASQUATTI, T. N.; SILVA-PEREIRA, T. T.; TAKATANI, H.; SILVA, C. B. D. G.; ABRAMOVITH, R. B.; GUIMARAES, A. M. S.; DAVILA, A. M. R.; ARAUJO, F. R.; KANEENE, J. B. Genetic Diversity and Potential Paths of Transmission of Mycobacterium bovis in the Amazon: the Discovery of M. bovis Lineage Lb1 Circulating in South America. Frontiers in Veterinary Science, v. 8, Article 630989, 2021.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| Registros recuperados : 13 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Unidades Centrais. |
|
Data corrente: |
19/03/2018 |
|
Data da última atualização: |
20/03/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
BIER, D.; TUTIJA, J. F.; PASQUATTI, T. N.; OLIVEIRA, T. L.; ARAUJO, F. R.; VERBISCK, N. V. |
|
Afiliação: |
Daniele Bier, FAMEZ/Universidade Federal de Mato Grosso do Sul - UFMS; Juliane F. Tutija, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; Taynara N. Pasquatti, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; Tayná L. Oliveira, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; FLABIO RIBEIRO ARAUJO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC. |
|
Título: |
Identificação por espectrometria de massa MALDI-TOF de Salmonella spp. e Escherichia coli isolados de carcaças bovinas. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 12, p. 1373-1379, dezembro 2017. |
|
Idioma: |
Inglês |
|
Notas: |
Título em inglês: MALDI-TOF mass spectrometry identification of Salmonella spp. and Escherichia coli isolated from bovine carcasses. |
|
Conteúdo: |
The aim of this study was to introduce matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry to improve the traditional microbiological method for the detection of Salmonella spp. and Escherichia coli in beef carcasses. Two hundred seventy samples from 90 beef carcasses were evaluated. The methodologies described in ISO 6579:2002 and in the Compendium of Methods for the Microbiological Examination of Foods were used for Salmonella spp. and E. coli isolation, respectively. MALDI-TOF analysis were performed on tryptone soya broth suspension isolates or directly from nutrient agar colonies, from the positive, inconclusive or negative biochemically tested samples for Salmonella and E. coli. Mass profiles were acquired on an Autoflex III SmartBeam MALDI-TOF mass spectrometer and the raw spectra were processed using the MALDI Biotyper software (Bruker Daltonics). According to the preliminary identification based on colony morphology and the biochemical reactions, seven isolates were positive for Salmonella spp. Through MALDI Biotyper these seven isolates were also classified as belonging to the genus Salmonella and further identified as S. enterica. Four isolates showing unusual phenotypic characteristics and inconclusive results in biochemical tests for Salmonella were identified as belonging to Citrobacter and Proteus genera after MALDI analysis. Regarding Escherichia coli, 37 were positive for species biochemical testing which MALDI Biotyper confirmed. MALDI-TOF methodology allowed rapid Salmonella spp. and E. coli identity confirmation and may be used to detect these microrganisms within bacterial isolates from beef carcasses. MenosThe aim of this study was to introduce matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry to improve the traditional microbiological method for the detection of Salmonella spp. and Escherichia coli in beef carcasses. Two hundred seventy samples from 90 beef carcasses were evaluated. The methodologies described in ISO 6579:2002 and in the Compendium of Methods for the Microbiological Examination of Foods were used for Salmonella spp. and E. coli isolation, respectively. MALDI-TOF analysis were performed on tryptone soya broth suspension isolates or directly from nutrient agar colonies, from the positive, inconclusive or negative biochemically tested samples for Salmonella and E. coli. Mass profiles were acquired on an Autoflex III SmartBeam MALDI-TOF mass spectrometer and the raw spectra were processed using the MALDI Biotyper software (Bruker Daltonics). According to the preliminary identification based on colony morphology and the biochemical reactions, seven isolates were positive for Salmonella spp. Through MALDI Biotyper these seven isolates were also classified as belonging to the genus Salmonella and further identified as S. enterica. Four isolates showing unusual phenotypic characteristics and inconclusive results in biochemical tests for Salmonella were identified as belonging to Citrobacter and Proteus genera after MALDI analysis. Regarding Escherichia coli, 37 were positive for species biochemical testing which MALDI Biotyper... Mostrar Tudo |
|
Palavras-Chave: |
MALDI–TOF; Slaughterhouse. |
|
Thesagro: |
Escherichia Coli; Salmonella spp. |
|
Thesaurus NAL: |
Beef carcasses; Enterobacteriales; Mass spectrometry. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174160/1/Identificacao-por-espectrometria-de-massa-MALDI-TOF.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174156/1/Identificacao-por-espectrometria-de-massa-MALDI-TOF.pdf
|
|
Marc: |
LEADER 02676naa a2200277 a 4500
001 2089384
005 2018-03-20
008 2017 bl uuuu u00u1 u #d
100 1 $aBIER, D.
245 $aIdentificação por espectrometria de massa MALDI-TOF de Salmonella spp. e Escherichia coli isolados de carcaças bovinas.$h[electronic resource]
260 $c2017
500 $aTítulo em inglês: MALDI-TOF mass spectrometry identification of Salmonella spp. and Escherichia coli isolated from bovine carcasses.
520 $aThe aim of this study was to introduce matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry to improve the traditional microbiological method for the detection of Salmonella spp. and Escherichia coli in beef carcasses. Two hundred seventy samples from 90 beef carcasses were evaluated. The methodologies described in ISO 6579:2002 and in the Compendium of Methods for the Microbiological Examination of Foods were used for Salmonella spp. and E. coli isolation, respectively. MALDI-TOF analysis were performed on tryptone soya broth suspension isolates or directly from nutrient agar colonies, from the positive, inconclusive or negative biochemically tested samples for Salmonella and E. coli. Mass profiles were acquired on an Autoflex III SmartBeam MALDI-TOF mass spectrometer and the raw spectra were processed using the MALDI Biotyper software (Bruker Daltonics). According to the preliminary identification based on colony morphology and the biochemical reactions, seven isolates were positive for Salmonella spp. Through MALDI Biotyper these seven isolates were also classified as belonging to the genus Salmonella and further identified as S. enterica. Four isolates showing unusual phenotypic characteristics and inconclusive results in biochemical tests for Salmonella were identified as belonging to Citrobacter and Proteus genera after MALDI analysis. Regarding Escherichia coli, 37 were positive for species biochemical testing which MALDI Biotyper confirmed. MALDI-TOF methodology allowed rapid Salmonella spp. and E. coli identity confirmation and may be used to detect these microrganisms within bacterial isolates from beef carcasses.
650 $aBeef carcasses
650 $aEnterobacteriales
650 $aMass spectrometry
650 $aEscherichia Coli
650 $aSalmonella spp
653 $aMALDI–TOF
653 $aSlaughterhouse
700 1 $aTUTIJA, J. F.
700 1 $aPASQUATTI, T. N.
700 1 $aOLIVEIRA, T. L.
700 1 $aARAUJO, F. R.
700 1 $aVERBISCK, N. V.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 12, p. 1373-1379, dezembro 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
