02676naa a2200277 a 450000100080000000500110000800800410001910000130006024501500007326000090022350001400023252016940037265000190206665000220208565000220210765000210212965000190215065300160216965300190218570000180220470000210222270000200224370000180226370000200228177300970230120893842018-03-20       2017    bl uuuu     u00u1 u    #d1 aBIER, D.  aIdentificação por espectrometria de massa MALDI-TOF de Salmonella spp. e Escherichia coli isolados de carcaças bovinas.h[electronic resource]  c2017  aTítulo em inglês:  MALDI-TOF mass spectrometry identification of Salmonella spp. and Escherichia coli isolated from bovine carcasses.  aThe aim of this study was to introduce matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry to improve the traditional microbiological method for the detection of Salmonella spp. and Escherichia coli in beef carcasses. Two hundred seventy samples from 90 beef carcasses were evaluated. The methodologies described in ISO 6579:2002 and in the Compendium of Methods for the Microbiological Examination of Foods were used for Salmonella spp. and E. coli isolation, respectively. MALDI-TOF analysis were performed on tryptone soya broth suspension isolates or directly from nutrient agar colonies, from the positive, inconclusive or negative biochemically tested samples for Salmonella and E. coli. Mass profiles were acquired on an Autoflex III SmartBeam MALDI-TOF mass spectrometer and the raw spectra were processed using the MALDI Biotyper software (Bruker Daltonics). According to the preliminary identification based on colony morphology and the biochemical reactions, seven isolates were positive for Salmonella spp. Through MALDI Biotyper these seven isolates were also classified as belonging to the genus Salmonella and further identified as S. enterica. Four isolates showing unusual phenotypic characteristics and inconclusive results in biochemical tests for Salmonella were identified as belonging to Citrobacter and Proteus genera after MALDI analysis. Regarding Escherichia coli, 37 were positive for species biochemical testing which MALDI Biotyper confirmed. MALDI-TOF methodology allowed rapid Salmonella spp. and E. coli identity confirmation and may be used to detect these microrganisms within bacterial isolates from beef carcasses.  aBeef carcasses  aEnterobacteriales  aMass spectrometry  aEscherichia Coli  aSalmonella spp  aMALDI–TOF  aSlaughterhouse1 aTUTIJA, J. F.1 aPASQUATTI, T. N.1 aOLIVEIRA, T. L.1 aARAUJO, F. R.1 aVERBISCK, N. V.  tPesquisa Veterinária Brasileira, Rio de Janeirogv. 37, n. 12, p. 1373-1379, dezembro 2017.