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| Registros recuperados : 9 | |
| 5. |  | JASMIM, J.; MARTINS, S. C.; GONÇALVES, F. S.; ASCARI, I. J.; CAMARGO, L. S. de A.; MENDEZ-OTERO, R. Development of bovine embryons in vitro in co-culture with mesenchymal stem cells and murine embryonic fibroblasts Animal Reproduction, v. 12, n. 3, p. 761. 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | JASMIN, J.; GONÇALVES, F. S.; ASCARI, I. J.; FONSECA, T. S.; CAMARGO, L. S. de A.; PETERS, V. M.; MENDEZ-OTERO, R. Optimization of in vitro development of mouse embryos using mesenchymal stem cells as feeder layers. Animal Reproduction, v. 11, n. 3, p. 463, 2014. Edição dos proceedings do 28º Annual Meeting of the Brazilian Embryo Technology Society, 2014, Natal.| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | CAMARGO, L. S. de A.; OTERO, R. J.; GONÇALVES, F. S.; MUNK, M. P.; SOUZA, E. D.; IGUMA, L. T.; TOLEDO, J. R.; PARRA, N. C.; QUINTAO, C. C. R.; VIANA, J. H. M. Effect of lentiviral vectors microinjection into the perivitelline space of bovine early zygotes on embryo development and transgene expression. In: TRANSGENIC ANIMAL RESEARCH CONFERENCE, 9., 2013, Tahoe City, California. Abstracts... Tahoe City: [s.n.], 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | CAMARGO, L. S. de A.; OTERO, R. J.; GONÇALVES, F. S.; MUNK, M. P.; SOUZA, E. D.; IGUMA, L. T.; TOLEDO, J. R.; PARRA PEREIRA, N. C.; QUINTAO, C. C. R.; VIANA, J. H. M. Effect of lentiviral vectors microinjection into the perivitelline space of bovine early zygotes on embryo development and transgene expression. In: UC Davis Transgenic Animal Research Conference, 9., 2014, Abstract...| Biblioteca(s): Embrapa Gado de Leite. |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
OTERO, R.; HERNÁNDEZ, D.; CAMARGO, L. S. de A. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Effect of Trichostatin-A on embryos of bovine clones modified genetically with GFP. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Indian Journal of Science and Technology, v. 11, n. 25, 2018. |
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Páginas: |
9 p. |
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DOI: |
10.17485/ijst%2F2018%2Fv11i25%2F128251 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Objective: To evaluate the effect of treatment with trichostatin-A (TSA) on the production of bovine embryos, expressing the gene of the green fluorescent protein (GFP) generated by SCNT. Materials: 164 oocytes were distributed in three treatments, NT-GFP: newly reconstructed zygotes with genetically modified cells and not subject to TSA. NT-Trico-GFP: newly reconstructed zygotes with genetically modified cells and subjected to TSA. PART: Zygotes generated by parthenogenetic activation, used as a control for the process of oocyte activation and culture of embryos. The rates of cleavage, blastocysts, and embryos that expressed GFP were assessed by contingency tables and chi-square tests. Results: The percentage of cleavage in the zygotes in the NT-GFP treatment was greater but did not vary significantly from the NT-Trico-GFP treatment. However, this last treatment had a higher percentage of blastocyst formation (p = 0.077). The percentage of blastocysts from cleaved zygotes, the produced embryos were significantly higher (p < 0.05) for the NT-Trico-GFP treatment than for the NT-GFP. In both treatments, all the blastocysts generated expressed the GFP protein. Conclusions:TSA improves the embryonic development of clones of genetically modified cattle that express GFP. |
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Palavras-Chave: |
Embryonic Development; Epigenetic Modification; Nuclear Transfer. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189328/1/Artigo-Indian-J-Sci-and-Tech-Camargo-Effect.pdf
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Marc: |
LEADER 01943naa a2200205 a 4500
001 2102530
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008 2018 bl uuuu u00u1 u #d
024 7 $a10.17485/ijst%2F2018%2Fv11i25%2F128251$2DOI
100 1 $aOTERO, R.
245 $aEffect of Trichostatin-A on embryos of bovine clones modified genetically with GFP.$h[electronic resource]
260 $c2018
300 $a9 p.
520 $aAbstract Objective: To evaluate the effect of treatment with trichostatin-A (TSA) on the production of bovine embryos, expressing the gene of the green fluorescent protein (GFP) generated by SCNT. Materials: 164 oocytes were distributed in three treatments, NT-GFP: newly reconstructed zygotes with genetically modified cells and not subject to TSA. NT-Trico-GFP: newly reconstructed zygotes with genetically modified cells and subjected to TSA. PART: Zygotes generated by parthenogenetic activation, used as a control for the process of oocyte activation and culture of embryos. The rates of cleavage, blastocysts, and embryos that expressed GFP were assessed by contingency tables and chi-square tests. Results: The percentage of cleavage in the zygotes in the NT-GFP treatment was greater but did not vary significantly from the NT-Trico-GFP treatment. However, this last treatment had a higher percentage of blastocyst formation (p = 0.077). The percentage of blastocysts from cleaved zygotes, the produced embryos were significantly higher (p < 0.05) for the NT-Trico-GFP treatment than for the NT-GFP. In both treatments, all the blastocysts generated expressed the GFP protein. Conclusions:TSA improves the embryonic development of clones of genetically modified cattle that express GFP.
653 $aEmbryonic Development
653 $aEpigenetic Modification
653 $aNuclear Transfer
700 1 $aHERNÁNDEZ, D.
700 1 $aCAMARGO, L. S. de A.
773 $tIndian Journal of Science and Technology$gv. 11, n. 25, 2018.
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Embrapa Gado de Leite (CNPGL) |
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