01943naa a2200205 a 450000100080000000500110000800800410001902400480006010000140010824501110012226000090023330000090024252013010025165300260155265300280157865300210160670000190162770000250164677300660167121025302023-01-24       2018    bl uuuu     u00u1 u    #d7 a10.17485/ijst%2F2018%2Fv11i25%2F1282512DOI1 aOTERO, R.  aEffect of Trichostatin-A on embryos of bovine clones modified genetically with GFP.h[electronic resource]  c2018  a9 p.  aAbstract Objective: To evaluate the effect of treatment with trichostatin-A (TSA) on the production of bovine embryos, expressing the gene of the green fluorescent protein (GFP) generated by SCNT. Materials: 164 oocytes were distributed in three treatments, NT-GFP: newly reconstructed zygotes with genetically modified cells and not subject to TSA. NT-Trico-GFP: newly reconstructed zygotes with genetically modified cells and subjected to TSA. PART: Zygotes generated by parthenogenetic activation, used as a control for the process of oocyte activation and culture of embryos. The rates of cleavage, blastocysts, and embryos that expressed GFP were assessed by contingency tables and chi-square tests. Results: The percentage of cleavage in the zygotes in the NT-GFP treatment was greater but did not vary significantly from the NT-Trico-GFP treatment. However, this last treatment had a higher percentage of blastocyst formation (p = 0.077). The percentage of blastocysts from cleaved zygotes, the produced embryos were significantly higher (p &lt 0.05) for the NT-Trico-GFP treatment than for the NT-GFP. In both treatments, all the blastocysts generated expressed the GFP protein. Conclusions:TSA improves the embryonic development of clones of genetically modified cattle that express GFP.  aEmbryonic Development  aEpigenetic Modification  aNuclear Transfer1 aHERNÁNDEZ, D.1 aCAMARGO, L. S. de A.  tIndian Journal of Science and Technologygv. 11, n. 25, 2018.