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| Registros recuperados : 193 | |
| 64. |  | PEREIRA, A. F.; MELO, P. G. S.; OLIVEIRA, J. P. de; ASSUNÇÃO, A.; BUENO, L. G. Qualidade fisiológica de sementes e desempenho agronômico de genótipos de milho doce. Pesquisa Agropecuária Tropical, Goiânia, v. 38, n. 4, p. 249-261, out./dez. 2008.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Hortaliças. |
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| 68. | | COSTA, J. G. C. da; WENDLAND, A.; DIAS, A. G. de; OLIVEIRA, J. P. de. Variedades tradicionais de feijão comum (Phaseolus vulgaris), com tipo de grão vermelho, resistentes à antracnose. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 7., 2013, Uberlândia. Variedade melhorada: a força da nossa agricultura: anais. Viçosa, MG: SBMP, 2013. p. 3201-3203.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 70. | | PEREIRA, S. A.; OLIVEIRA, G. C. de; SEVERIANO, E. da C.; BALBINO, L. C.; OLIVEIRA, J. P. de. Análise de componentes principais dos atributos físicos de um Latossolo Vermelho Distrófico típico sob pastagem e mata. Global Science and Technology, Rio Verde, v. 3, n. 2, p. 87-97, maio/ago. 2010.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 71. | | OLIVEIRA, E. B. de; OLIVEIRA, J. P. de; SCHMITZ, G.; ALVES, L. da S.; PEREIRA, J. E. S. Aspectos nutricionais do meio e influência da divisão longitudinal da brotação sobre o enraizamento in vitro de cultivares de bananeira. In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 16.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 3.; SIMPÓSIO DE PLANTAS ORNAMENTAIS NATIVAS, 1., 2007, Goiânia. Biodiversidade e competitividade: buscando novas opções: anais. Campinas: Sociedade Brasileira de Floricultura e Plantas Ornamentais, 2007. p. 884-887.| Biblioteca(s): Embrapa Acre. |
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| 72. | | GUEDES, R. da S.; SCHMITZ, G. C. B.; MACIEL, S. de A.; OLIVEIRA, J. P. de; PEREIRA, J. E. S. Avaliação da germinação de sementes e do desenvolvimento inicial de plantas de pimenta longa in vitro. Horticultura Brasileira, v. 24, n. 1, p. 41-44, jul. 2006. Suplemento. 1 CD ROM. Edição dos anais do 46º Congresso Brasileiro de Olericultura, Goiânia, 2006.| Biblioteca(s): Embrapa Acre. |
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| 75. | | OLIVEIRA, J. P. de; SANTOS, A. T. dos; MATTOS, P. P. de; CANETTI, A.; BRAZ, E. M. Competição espacial e crescimento de Ocotea odorifera (Vell.) Rohwer em remanescente de Floresta Ombrófila Mista. Nativa, Sinop, v. 10, n. 4, p. 458-465, 2022.| Biblioteca(s): Embrapa Florestas. |
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| 78. | | MACIEL, S. de A.; OLIVEIRA, J. P. de; SILVA, D. A. da; TEIXEIRA, R. B.; RAPOSO, A. Germinação de sementes de seringueira in vitro a partir de embriões zigóticos. In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 18.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 5., 2011, Joinville. Inovar com sustentabilidade: palestras, posters, minicursos. Itajaí: ABCTP: SBFP, 2011. 2 p.| Biblioteca(s): Embrapa Acre. |
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| 80. | | OLIVEIRA, J. P. de; CHAVES, L. J.; DUARTE, J. B.; RIBEIRTO, K. de O.; BRASIL, E. M. Heterosis for oil content in maize populations and hybrids of high quality protein. Crop breeding and applied biotechnology, Londrina, v. 6, n. 2, p. 113-120, June 2006.| Biblioteca(s): Embrapa Agricultura Digital. |
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| Registros recuperados : 193 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Unidades Centrais. |
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Data corrente: |
19/03/2018 |
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Data da última atualização: |
20/03/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BIER, D.; TUTIJA, J. F.; PASQUATTI, T. N.; OLIVEIRA, T. L.; ARAUJO, F. R.; VERBISCK, N. V. |
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Afiliação: |
Daniele Bier, FAMEZ/Universidade Federal de Mato Grosso do Sul - UFMS; Juliane F. Tutija, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; Taynara N. Pasquatti, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; Tayná L. Oliveira, Curso de Medicina Veterinária/Universidade Católica Dom Bosco - UCDB; FLABIO RIBEIRO ARAUJO, CNPGC; NEWTON VALERIO VERBISCK, CNPGC. |
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Título: |
Identificação por espectrometria de massa MALDI-TOF de Salmonella spp. e Escherichia coli isolados de carcaças bovinas. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 12, p. 1373-1379, dezembro 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em inglês: MALDI-TOF mass spectrometry identification of Salmonella spp. and Escherichia coli isolated from bovine carcasses. |
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Conteúdo: |
The aim of this study was to introduce matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry to improve the traditional microbiological method for the detection of Salmonella spp. and Escherichia coli in beef carcasses. Two hundred seventy samples from 90 beef carcasses were evaluated. The methodologies described in ISO 6579:2002 and in the Compendium of Methods for the Microbiological Examination of Foods were used for Salmonella spp. and E. coli isolation, respectively. MALDI-TOF analysis were performed on tryptone soya broth suspension isolates or directly from nutrient agar colonies, from the positive, inconclusive or negative biochemically tested samples for Salmonella and E. coli. Mass profiles were acquired on an Autoflex III SmartBeam MALDI-TOF mass spectrometer and the raw spectra were processed using the MALDI Biotyper software (Bruker Daltonics). According to the preliminary identification based on colony morphology and the biochemical reactions, seven isolates were positive for Salmonella spp. Through MALDI Biotyper these seven isolates were also classified as belonging to the genus Salmonella and further identified as S. enterica. Four isolates showing unusual phenotypic characteristics and inconclusive results in biochemical tests for Salmonella were identified as belonging to Citrobacter and Proteus genera after MALDI analysis. Regarding Escherichia coli, 37 were positive for species biochemical testing which MALDI Biotyper confirmed. MALDI-TOF methodology allowed rapid Salmonella spp. and E. coli identity confirmation and may be used to detect these microrganisms within bacterial isolates from beef carcasses. MenosThe aim of this study was to introduce matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry to improve the traditional microbiological method for the detection of Salmonella spp. and Escherichia coli in beef carcasses. Two hundred seventy samples from 90 beef carcasses were evaluated. The methodologies described in ISO 6579:2002 and in the Compendium of Methods for the Microbiological Examination of Foods were used for Salmonella spp. and E. coli isolation, respectively. MALDI-TOF analysis were performed on tryptone soya broth suspension isolates or directly from nutrient agar colonies, from the positive, inconclusive or negative biochemically tested samples for Salmonella and E. coli. Mass profiles were acquired on an Autoflex III SmartBeam MALDI-TOF mass spectrometer and the raw spectra were processed using the MALDI Biotyper software (Bruker Daltonics). According to the preliminary identification based on colony morphology and the biochemical reactions, seven isolates were positive for Salmonella spp. Through MALDI Biotyper these seven isolates were also classified as belonging to the genus Salmonella and further identified as S. enterica. Four isolates showing unusual phenotypic characteristics and inconclusive results in biochemical tests for Salmonella were identified as belonging to Citrobacter and Proteus genera after MALDI analysis. Regarding Escherichia coli, 37 were positive for species biochemical testing which MALDI Biotyper... Mostrar Tudo |
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Palavras-Chave: |
MALDI–TOF; Slaughterhouse. |
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Thesagro: |
Escherichia Coli; Salmonella spp. |
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Thesaurus NAL: |
Beef carcasses; Enterobacteriales; Mass spectrometry. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174160/1/Identificacao-por-espectrometria-de-massa-MALDI-TOF.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174156/1/Identificacao-por-espectrometria-de-massa-MALDI-TOF.pdf
|
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Marc: |
LEADER 02676naa a2200277 a 4500
001 2089384
005 2018-03-20
008 2017 bl uuuu u00u1 u #d
100 1 $aBIER, D.
245 $aIdentificação por espectrometria de massa MALDI-TOF de Salmonella spp. e Escherichia coli isolados de carcaças bovinas.$h[electronic resource]
260 $c2017
500 $aTítulo em inglês: MALDI-TOF mass spectrometry identification of Salmonella spp. and Escherichia coli isolated from bovine carcasses.
520 $aThe aim of this study was to introduce matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry to improve the traditional microbiological method for the detection of Salmonella spp. and Escherichia coli in beef carcasses. Two hundred seventy samples from 90 beef carcasses were evaluated. The methodologies described in ISO 6579:2002 and in the Compendium of Methods for the Microbiological Examination of Foods were used for Salmonella spp. and E. coli isolation, respectively. MALDI-TOF analysis were performed on tryptone soya broth suspension isolates or directly from nutrient agar colonies, from the positive, inconclusive or negative biochemically tested samples for Salmonella and E. coli. Mass profiles were acquired on an Autoflex III SmartBeam MALDI-TOF mass spectrometer and the raw spectra were processed using the MALDI Biotyper software (Bruker Daltonics). According to the preliminary identification based on colony morphology and the biochemical reactions, seven isolates were positive for Salmonella spp. Through MALDI Biotyper these seven isolates were also classified as belonging to the genus Salmonella and further identified as S. enterica. Four isolates showing unusual phenotypic characteristics and inconclusive results in biochemical tests for Salmonella were identified as belonging to Citrobacter and Proteus genera after MALDI analysis. Regarding Escherichia coli, 37 were positive for species biochemical testing which MALDI Biotyper confirmed. MALDI-TOF methodology allowed rapid Salmonella spp. and E. coli identity confirmation and may be used to detect these microrganisms within bacterial isolates from beef carcasses.
650 $aBeef carcasses
650 $aEnterobacteriales
650 $aMass spectrometry
650 $aEscherichia Coli
650 $aSalmonella spp
653 $aMALDI–TOF
653 $aSlaughterhouse
700 1 $aTUTIJA, J. F.
700 1 $aPASQUATTI, T. N.
700 1 $aOLIVEIRA, T. L.
700 1 $aARAUJO, F. R.
700 1 $aVERBISCK, N. V.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 12, p. 1373-1379, dezembro 2017.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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