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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia; Embrapa Agroindústria de Alimentos. |
Data corrente: |
19/09/2017 |
Data da última atualização: |
08/12/2017 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
TAMIETTI, M. S.; MIDORIKAWA, G. E. O.; MENDES, T. D.; DAMASO, M. C. T.; BOM, E. P. S.; SILVA, A. S.; GOTTSCHALK, L. M. F.; NORONHA, E. F.; FAVARO, L. C. de L. |
Afiliação: |
MARIANA S. TAMIETTI, UNB; GLÁUCIA E. O. MIDORIKAWA; THAIS DEMARCHI MENDES, CNPAE; MONICA CARAMEZ TRICHES DAMASO, CNPAE; ELBA P. S. BOM, UFRJ; AYLA S. SILVA, INT; LEDA MARIA FORTES GOTTSCHALK, CTAA; ELIANE F. NORONHA, UNB; LEIA CECILIA DE LIMA FAVARO, CNPAE. |
Título: |
Enhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracaju, SE. [Proceedings ...]. São Paulo: Associação Brasileira de Engenharia Química, 2017. |
Páginas: |
Não paginado. |
ISSN: |
2447-2816 |
Idioma: |
Inglês |
Notas: |
SINAFERM; SHEB. 3 a 6 de setembro. Seção Trabalhos. Ref. 59019. |
Conteúdo: |
Carbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined and compared. All genetically stable transformants showed increased enzymatic index under inductive conditions and modest inhibition under repressive conditions for most carbon sources, indicating that the deletion of cre1 in T. harzianum can be beneficial to cellulase and hemicellulase production with reduced product inhibition. |
Palavras-Chave: |
Carbon Catabolite Repression; Cellulolytic and hemicellulolytic enzymes; Processos de hidrolise enzimática de biomassa. |
Thesagro: |
Celulase; Trichoderma Harzianum. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/164843/1/galoa-proceedings-sinaferm-2017-59019-enhancement-of-t.pdf
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Marc: |
LEADER 02302nam a2200301 a 4500 001 2076994 005 2017-12-08 008 2017 bl uuuu u00u1 u #d 022 $a2447-2816 100 1 $aTAMIETTI, M. S. 245 $aEnhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1.$h[electronic resource] 260 $aIn: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracaju, SE. [Proceedings ...]. São Paulo: Associação Brasileira de Engenharia Química$c2017 300 $aNão paginado. 500 $aSINAFERM; SHEB. 3 a 6 de setembro. Seção Trabalhos. Ref. 59019. 520 $aCarbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined and compared. All genetically stable transformants showed increased enzymatic index under inductive conditions and modest inhibition under repressive conditions for most carbon sources, indicating that the deletion of cre1 in T. harzianum can be beneficial to cellulase and hemicellulase production with reduced product inhibition. 650 $aCelulase 650 $aTrichoderma Harzianum 653 $aCarbon Catabolite Repression 653 $aCellulolytic and hemicellulolytic enzymes 653 $aProcessos de hidrolise enzimática de biomassa 700 1 $aMIDORIKAWA, G. E. O. 700 1 $aMENDES, T. D. 700 1 $aDAMASO, M. C. T. 700 1 $aBOM, E. P. S. 700 1 $aSILVA, A. S. 700 1 $aGOTTSCHALK, L. M. F. 700 1 $aNORONHA, E. F. 700 1 $aFAVARO, L. C. de L.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
03/06/2020 |
Data da última atualização: |
28/06/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 3 |
Autoria: |
COSTA, J. N. da; LEAL, A. R.; NASCIMENTO, L. G. L.; RODRIGUES, D. C.; MUNIZ, C. R.; FIGUEIREDO, R. W.; MATA, P.; NORONHA, J. P.; SOUSA, P. H. M. de. |
Afiliação: |
JULIANA N. DA COSTA, Departament of Food Engineering Federal University of Cear; AMANDA R. LEAL, Departament of Food Engineering Federal University of Cear; LUIS G. L. NASCIMENTO, Departament of Food Engineering Federal University of Ceara; DELANE C. RODRIGUES, Departament of Food Engineering Federal University of Ceara; CELLI RODRIGUES MUNIZ, CNPAT; RAIMUNDO W. FIGUEIREDO, Departament of Food Engineering Federal University of Ceara; PAULINA MATA, LAQV, REQUIMTE, Departament of Chemistry, Faculty of Science and Technology, New University of Lisbon; JOÃO PAULO NORONHA, LAQV, REQUIMTE, Departament of Chemistry, Faculty of Science and Technology, New University of Lisbon; PAULO HENRIQUE M. DE SOUSA, Departament of Food Engineering Federal University of Ceara. |
Título: |
Texture, microstructure and volatile profile of structured guava using agar and gellan gum. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
International Journal of Gastronomy and Food Science, 20, art. no. 100207, 2020. |
DOI: |
https://doi.org/10.1016/j.ijgfs.2020.100207 |
Idioma: |
Inglês |
Palavras-Chave: |
(COV); (VOCs); Compostos orgânicos voláteis; Frutas estruturadas; Goma gelana; Hidrocolóides; Microscopia eletrônica de varredura; Structured fruit; Texturometria; Texturometry. |
Thesagro: |
Agar. |
Thesaurus NAL: |
Gellan gum; Hydrocolloids; Scanning electron microscopy; Volatile organic compounds. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01218naa a2200397 a 4500 001 2122970 005 2020-06-28 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.ijgfs.2020.100207$2DOI 100 1 $aCOSTA, J. N. da 245 $aTexture, microstructure and volatile profile of structured guava using agar and gellan gum.$h[electronic resource] 260 $c2020 650 $aGellan gum 650 $aHydrocolloids 650 $aScanning electron microscopy 650 $aVolatile organic compounds 650 $aAgar 653 $a(COV) 653 $a(VOCs) 653 $aCompostos orgânicos voláteis 653 $aFrutas estruturadas 653 $aGoma gelana 653 $aHidrocolóides 653 $aMicroscopia eletrônica de varredura 653 $aStructured fruit 653 $aTexturometria 653 $aTexturometry 700 1 $aLEAL, A. R. 700 1 $aNASCIMENTO, L. G. L. 700 1 $aRODRIGUES, D. C. 700 1 $aMUNIZ, C. R. 700 1 $aFIGUEIREDO, R. W. 700 1 $aMATA, P. 700 1 $aNORONHA, J. P. 700 1 $aSOUSA, P. H. M. de 773 $tInternational Journal of Gastronomy and Food Science, 20, art. no. 100207, 2020.
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