02302nam a2200301 a 450000100080000000500110000800800410001902200140006010000200007424501740009426002060026830000190047450000700049352011010056365000130166465000260167765300330170365300460173665300510178270000250183370000180185870000210187670000180189770000170191570000250193270000190195770000240197620769942017-12-08       2017    bl uuuu     u00u1 u    #d  a2447-28161 aTAMIETTI, M. S.  aEnhancement of Trichoderma harzianum CFAM-422 for cellulase and hemicellulase production by deletion of the carbon catabolite repressor gene cre1.h[electronic resource]  aIn: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 21.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 12., 2017, Aracaju, SE. [Proceedings ...]. São Paulo: Associação Brasileira de Engenharia Químicac2017  aNão paginado.  aSINAFERM; SHEB. 3 a 6 de setembro. Seção Trabalhos. Ref. 59019.  aCarbon catabolite repression (CCR) is a mechanism by which microorganisms can utilize preferably highly energetic compounds over those of difficult degradation. For Trichoderma reesei, the protein that acts as repressor in the presence of glucose is CRE1. In this project, we aim to delete cre1 gene in Trichoderma harzianum CFAM-422 and obtain mutants with enhanced production of biomass degrading enzymes. Disruption of cre1 in T. harzianum CFAM-422 was performed by gene replacement of cre1 for hph (hygromycin B phosphotransferase) via homologous recombination. Hygromycin resistant mutants and parental strains enzyme production was evaluated in both inductive and repressive conditions in four different carbon sources. Enzymatic indexes (EI) were determined and compared. All genetically stable transformants showed increased enzymatic index under inductive conditions and modest inhibition under repressive conditions for most carbon sources, indicating that the deletion of cre1 in T. harzianum can be beneficial to cellulase and hemicellulase production with reduced product inhibition.  aCelulase  aTrichoderma Harzianum  aCarbon Catabolite Repression  aCellulolytic and hemicellulolytic enzymes  aProcessos de hidrolise enzimática de biomassa1 aMIDORIKAWA, G. E. O.1 aMENDES, T. D.1 aDAMASO, M. C. T.1 aBOM, E. P. S.1 aSILVA, A. S.1 aGOTTSCHALK, L. M. F.1 aNORONHA, E. F.1 aFAVARO, L. C. de L.