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4. | | MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis as a model plant for functional genomic studies in C4 Crops. In: KUMAR, S.; BARONE, P.; SMITH, M. Transgenic plants: methods and protocols. Berlim, Alemanha: Springer Science+Business Media, 2019. (Methods in Molecular Biology, v. 1864). p. 49-64 Biblioteca(s): Embrapa Agroenergia. |
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5. | | OYA, T.; MARTINS, P. K.; CASAGRANDE, E. C.; LUGLE, S. M.; NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J. R. B. Agronomic, physiological and molecular characteristics of drought-tolerant Brazilian soybean cultivars. In: EMBRAPA SOYBEAN/JICA-CETAPAR/INTA-EEA/JIRCAS JOINT WORKSHOP ON SOYBEAN IMPROVEMENT, PRODUCTION AND UTILIZATION IN SOUTH AMERICA, 2001, Londrina. Abstracts and program. Londrina: Embrapa Soybean: JIRCAS, 2001. p. 17. (Embrapa Soja. Documentos, 170). Biblioteca(s): Embrapa Soja. |
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6. | | RIBEIRO, A. P.; MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; ANDRADE, A. C.; MOLINARI, H. B. C. Overexpression of the MATE gene in Setaria viridis. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 11. Biblioteca(s): Embrapa Agroenergia. |
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7. | | MARTINS, P. K.; DIAS, B. B. A.; RIBEIRO, A. P.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis: a tool for functional gene analysis in sugarcane. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 19. Biblioteca(s): Embrapa Agroenergia. |
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10. | | MAFRA, V.; MARTINS, P. K.; FRANSCISCO, C. S.; RIBEIRO-ALVES, M.; ASTUA, J. de F.; MACHADO, M. A. Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype. In; INTERNATIONAL RESEARCH CONFERENCE ON HUANGLONGBING , 3., 2013, Orlando, Flórida, USA. IRC HLB III. Proceedings. Orlando, Flórida, USA, 2013, (online). Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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12. | | MARTINS, P. K.; DIAS, B. B. A.; RIBEIRO, A. P.; SOUTO, B. de M.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Isolation, in silico analysis and transformation of Setaria viridis with a sugarcane senescence-associated promoter. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 25. Biblioteca(s): Embrapa Agroenergia. |
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13. | | SOUSA, C. A. F. de; DIAS, B. B. A.; MARTINS, P. K.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; SOUZA JUNIOR, M. T. Nova abordagem para a fenotipagem de plantas: conceitos, ferramentas e perspectivas. Revista Brasileira de Geografia Física, v. 8, p. 660-672, 2015. Biblioteca(s): Embrapa Agroenergia. |
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14. | | DIAS, B. B. A.; KOBAYASHI, A. K.; GOMES, A. de P. G.; RODRIGUES, D. de S.; MOLINARI, H. B. C.; FAVARO, L. C. de L.; MARTINS, P. K. Manual de Biossegurança da Embrapa Agroenergia (CQB 345/12). Brasília, DF: Embrapa Agroenergia, 2015. 35 p. ( Embrapa Agroenergia. Documentos, 19). Biblioteca(s): Embrapa Agroenergia; Embrapa Unidades Centrais. |
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15. | | MILORI, D. M. B. P.; BOAS, P. R. V.; VEMÂNCIO, A. L.; FERREIRA, E. J.; MARTINS, P. K.; FREITAS-ASTUA, J.; BRESOLIN, J.; CARDINALI, M. C. B. Diagnóstico de Citrus Greening (HLB) utilizando espectroscopia de fluorescência induzida por laser (LIFS). In: INAMASU, R. Y.; NAIME, J. de M.; RESENDE, A. V. de; BASSOI, L. H.; BERNARDI, A. de C. (Ed.). Agricultura de precisão: um novo olhar. São Carlos, SP: Embrapa Instrumentação, 2011. p. 78-81. Biblioteca(s): Embrapa Instrumentação. |
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16. | | MARTINS, P. K.; JORDÃO, B. Q.; YAMANAKA, N.; FARIAS, J. R. B.; BENEVENTI, M. A.; BINNECK, E.; FUGANTI, R.; STOLF, R.; NEPOMUCENO, A. L. Differential gene expression and mitotic cell analysis of the drought tolerant soybean (Glycine max L. Merrill Fabales, Fabaceae) cultivar MG/BR46 (Conquista) under two water deficit induction systems. Genetics and Molecular Biology, Ribeirão Preto, v. 31, n. 2, p. 512-521, 2008. Biblioteca(s): Embrapa Soja. |
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17. | | PEDROSO, J. C.; NEPOMUCENO, A. L.; SILVA, J. F. V.; CARNEIRO, N. P.; MARTINS, P. K.; BRETON, M. C.; MARIN, S. R. R.; BINNECK, E. Differential gene expression in nematode resistant soybean genotypes. Nematology, Boston, v. 4, n. 2, p. 235, 2002. Presented in Fourth International Congress of Nematology. Programme and abstracts. 8-13 June 2002, Tenbel, La Galletas, Arona, Tenerife, Canary Islands, Spain. Biblioteca(s): Embrapa Soja. |
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18. | | CORREA, P. H.; MARTINS, P. K.; ARENA, G. D.; MIGUEL, M. C.; MUTTI, H. S.; MACHADO, M. A.; FREITAS-ASTUA, J. Diagnóstico molecular de huanglongbing dos citros, via qPCR, em mudas de valência sobre Swingle. In:CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: Sociedade Brasileira de Fruticultura, 2010. pdf 1867 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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19. | | Ribeiro, A. P.; Martins, P. K.; DIAS, B. B. A.; ANDRADE, A. C.; MAGALHAES, J. V. de; KOBAYASHI, A. K.; MOLINARI, H. B. C. MATE: a promising gene for aluminum tolerance in sugarcane. In: GERMPLASM AND BREEDING, 11.; MOLECULAR BIOLOGY ISSCT WORKSHOP, 8., 2015, Saint-Gilles Réunion Island. Pushing the frontiers of sugarcane improvement: abstract. [S.l]: Ercane, 2015. Biblioteca(s): Embrapa Agroenergia. |
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20. | | MARTINS, P. K.; NAKAYAMA, T. J.; RIBEIRO, A. P.; DIAS, B. B. A.; NEPOMUCENO, A. L.; HARMON, F. G.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis floral-dip: a simple and rapid Agrobacterium-mediated transformation method. Biotechnology Reports, v. 6, p. 61-63, 2015. Biblioteca(s): Embrapa Agroenergia. |
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Registros recuperados : 77 | |
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Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
20/12/2010 |
Data da última atualização: |
19/01/2011 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
LOCALI-FABRIS, E. C.; MAFRA, V. S.; MARTINS, P. K.; RIBEIRO- ALVES, M.; FRANCISCO, C. S.; FREITAS ASTUA, J. de; KISHI, L. T.; MACHADO, M. A. |
Afiliação: |
Eliane Cristina Locali-Fabris, APTA; Valéria S. Mafra, APTA; Polyana Kelly. Martins, APTA; Marcelo Ribeiro-Alves, Fiocruz; Carolina S. Francisco, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Luciano T. Kishi, APTA; Marcelo A. Machado, APTA. |
Título: |
Gene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. |
Idioma: |
Inglês |
Notas: |
0058 PSO
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 |
Conteúdo: |
Huanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, including oxygen binding, monooxygenase activity and oxidoreductase activity, 6 cellular components, mostly related to chloroplast, enriched at a statistically significant level of 0.001. MenosHuanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, includin... Mostrar Tudo |
Palavras-Chave: |
Huanglongbing; Plant disease. |
Thesagro: |
Doença de Planta; Fruta Cítrica. |
Thesaurus NAL: |
Candidatus Liberibacter; Citrus. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02656nam a2200277 a 4500 001 1870461 005 2011-01-19 008 2010 bl uuuu u00u1 u #d 100 1 $aLOCALI-FABRIS, E. C. 245 $aGene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus. 260 $aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.$c2010 500 $a0058 PSO Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 520 $aHuanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, including oxygen binding, monooxygenase activity and oxidoreductase activity, 6 cellular components, mostly related to chloroplast, enriched at a statistically significant level of 0.001. 650 $aCandidatus Liberibacter 650 $aCitrus 650 $aDoença de Planta 650 $aFruta Cítrica 653 $aHuanglongbing 653 $aPlant disease 700 1 $aMAFRA, V. S. 700 1 $aMARTINS, P. K. 700 1 $aRIBEIRO- ALVES, M. 700 1 $aFRANCISCO, C. S. 700 1 $aFREITAS ASTUA, J. de 700 1 $aKISHI, L. T. 700 1 $aMACHADO, M. A.
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