02656nam a2200277 a 450000100080000000500110000800800410001910000250006024501040008526001440018950001050033352016830043865000280212165000110214965000220216065000190218265300180220165300180221970000170223770000190225470000230227370000210229670000250231770000170234270000190235918704612011-01-19       2010    bl uuuu     u00u1 u    #d1 aLOCALI-FABRIS, E. C.  aGene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus.  aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.c2010  a0058 PSO Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010  aHuanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, including oxygen binding, monooxygenase activity and oxidoreductase activity, 6 cellular components, mostly related to chloroplast, enriched at a statistically significant level of 0.001.  aCandidatus Liberibacter  aCitrus  aDoença de Planta  aFruta Cítrica  aHuanglongbing  aPlant disease1 aMAFRA, V. S.1 aMARTINS, P. K.1 aRIBEIRO- ALVES, M.1 aFRANCISCO, C. S.1 aFREITAS ASTUA, J. de1 aKISHI, L. T.1 aMACHADO, M. A.