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| Registros recuperados : 77 | |
| 8. |  | DIAS, L. T.; McMANUS, C. SASAKI, L. C. B.; LUCCI, C.; GARCIA, J. A.; LOUVANDINI, H. Análise comparativa de características da pele e pelame relacionadas à adaptação ao calor em ovinos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 44., 2007, Jaboticabal. O avanço científico e tecnológico na produção animal: anais. Jaboticabal: Sociedade Brasileira de Zootecnia: UNESP, Faculdade de Ciências Agrárias e Veterinárias, 2007. 3 f. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | GOMES, E. F.; MCMANUS, C.; LOUVANDINI, H.; PAIVA, S. R.; DALLAGO, B. S. Análise genealógica de um núcleo de conservação de ovinos bergamácia. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. CD-ROM| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | LANDIM, A. V.; PASCOA, L.; McMANUS, C.; LOUVANDINI, H.; MARIANTE, A. da S. Aspectos quantitativos em carcaças de cordeiros da raça Santa Inês puros e seus cruzamentos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 44., 2007, Jaboticabal. O avanço científico e tecnológico na produção animal: anais. Jaboticabal: Sociedade Brasileira de Zootecnia: UNESP, Faculdade de Ciências Agrárias e Veterinárias, 2007. 3 p.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | ROMAGNOLI, E. M.; DUNLAP, C.; LOUVANDINI, H.; TSAI, S. M.; ABDALLA, A. L.; MENDES, R. Dynamics of sheep rumen microbiome and its relationship with the degradation of biomass. In: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 16., 2016, Montreal. Proceedings... Wageningen: The International Society for Microbial Ecology (ISME), 2016. p. 830.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 14. | | McMANUS, C.; EGITO, A. A.; MORETZSOHN, M. C.; SOARES F, G.; FALCÃO, R. A.; LOUVANDINI, H. Growth of Saanen, Alpine and Toggenburg goatas in the Federal District, Brazil: genetic and environmental factors. In: WORLD CONFERENCE ON ANIMAL PRODUCTION, 9.; REUNIÃO DA ASSOCIAÇÃO LATIONOAMERICANA DE PRODUÇÃO ANIMAL, 18., Porto Alegre. Proceedings: contributed papers-abstracts. Porto Alegre: World Association of Animal Production: ALPA: SBZ: UFRGS, 2003. Seção 1-A, p. 91.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | LANDIM, A. V.; CARDOSO, M. T.; CASTANHEIRA, M.; FIORAVANTI, M. C.; LOUVANDINI, H.; McMANUS, C. Fatty acid profile of hair lambs and their crossbreds slaughtered at different weights. Tropical Animal Health and Production, v. 43, n. 8, p. 1561-1566, Dec. 2011.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | SANTOS, V. R. V. dos; LOUVANDINI, H.; PIMENTEL, C. M. M.; BRITO, D. L. Características estruturais e bromatológicas do capim Tanzânia sob pastejo isolado, simultâneo e alternado de ovinos com bovinos. Ciência Animal Brasileira, Goiânia, v. 12, n. 4, p. 670-680, out./dez. 2011.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 19. | | CARNEIRO, H.; MCMANUS, C.; LOUVANDINI, H.; PAIVA, S. R.; MACEDO, F.; MERNIES, B. Caracterização morfológica de ovinos no Brasil, Uruguai e Colômbia. SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia: Fundação de Apoio à Pesquisa Científica e Tecnológica - FUNCREDI, 2008. p.479.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | MCMANUS, C.; PAIVA, S. R.; EGITO, A. A. do; MARIANTE, A. da S.; LOUVANDINI, H. Importância dos levantamentos populacionais e da caracterização genética das populações na conservação animal. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 16., 2005, Goiânia, GO. [Anais...]. [Belo Horizonte: CBRA], 2005. Não paginado.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 77 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
04/01/2016 |
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Data da última atualização: |
10/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
LOPES, L. D.; LIMA, A. O. S.; TAKETANI, R. G.; DARIAS, P.; SILVA, L. R. F.; ROMAGNOLI, E. M.; LOUVANDINI, H.; ABDALLA, A. L.; MENDES, R. |
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Afiliação: |
LUCAS DANTAS LOPES; ANDRE OLIVEIRA DE SOUZA LIMA, UNIVALI; RODRIGO GOUVEA TAKETANI; PHILLIP DARIAS, UNIVALI; LILIA RAQUEL FE DA SILVA, UFPI; EMILIANA MANESCO ROMAGNOLI; HELDER LOUVANDINI, CENA-USP; ADIBE LUIZ ABDALLA, CENA-USP; RODRIGO MENDES, CNPMA. |
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Título: |
Exploring the sheep rumen microbiome for carbohydrate-active enzymes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Antonie van Leeuwenhoek, Amsterdam, v. 108, n. 1, p. 15-30, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) representing 12 glycosyl hydrolase families (Pfam database). Overall, we identified a total of 28 lignocellulases, 22 amylases and 9 other putative CAE, showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes MenosAbstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) represen... Mostrar Tudo |
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Palavras-Chave: |
Ion torrent (PGM). |
|
Thesagro: |
Amilase; Bactéria; Enzima; Ovelha; Rúmen. |
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Thesaurus NAL: |
Amylases; Bacterial communities; Lignocellulose; Metagenomics; Phylogeny. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02652naa a2200349 a 4500
001 2032734
005 2016-03-10
008 2015 bl uuuu u00u1 u #d
100 1 $aLOPES, L. D.
245 $aExploring the sheep rumen microbiome for carbohydrate-active enzymes.$h[electronic resource]
260 $c2015
520 $aAbstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) representing 12 glycosyl hydrolase families (Pfam database). Overall, we identified a total of 28 lignocellulases, 22 amylases and 9 other putative CAE, showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes
650 $aAmylases
650 $aBacterial communities
650 $aLignocellulose
650 $aMetagenomics
650 $aPhylogeny
650 $aAmilase
650 $aBactéria
650 $aEnzima
650 $aOvelha
650 $aRúmen
653 $aIon torrent (PGM)
700 1 $aLIMA, A. O. S.
700 1 $aTAKETANI, R. G.
700 1 $aDARIAS, P.
700 1 $aSILVA, L. R. F.
700 1 $aROMAGNOLI, E. M.
700 1 $aLOUVANDINI, H.
700 1 $aABDALLA, A. L.
700 1 $aMENDES, R.
773 $tAntonie van Leeuwenhoek, Amsterdam$gv. 108, n. 1, p. 15-30, 2015.
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