02652naa a2200349 a 450000100080000000500110000800800410001910000170006024500970007726000090017452017310018365000130191465000260192765000190195365000170197265000140198965000120200365000140201565000110202965000110204065000110205165300220206270000190208470000200210370000150212370000200213870000210215870000190217970000190219870000150221777300700223220327342016-03-10       2015    bl uuuu     u00u1 u    #d1 aLOPES, L. D.  aExploring the sheep rumen microbiome for carbohydrate-active enzymes.h[electronic resource]  c2015  aAbstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) representing 12 glycosyl hydrolase families (Pfam database). Overall, we identified a total of 28 lignocellulases, 22 amylases and 9 other putative CAE, showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes  aAmylases  aBacterial communities  aLignocellulose  aMetagenomics  aPhylogeny  aAmilase  aBactéria  aEnzima  aOvelha  aRúmen  aIon torrent (PGM)1 aLIMA, A. O. S.1 aTAKETANI, R. G.1 aDARIAS, P.1 aSILVA, L. R. F.1 aROMAGNOLI, E. M.1 aLOUVANDINI, H.1 aABDALLA, A. L.1 aMENDES, R.  tAntonie van Leeuwenhoek, Amsterdamgv. 108, n. 1, p. 15-30, 2015.