|
|
Registros recuperados : 65 | |
9. | | MAIA, A. de H. N.; SOUSA, K. K. A.; SILVA, R. B. Q. da; VIVIAN, R. Modelo de Cox com preditores qualitativos para análise de padrões temporais de mudança de fase em insetos. In: SIMPÓSIO NACIONAL DE PROBABILIDADE E ESTATÍSTICA - SINAPE, 23., 2018, São Pedro, SP. Resumos Expandidos. São Pedro, SP: ABE, 2018. Biblioteca(s): Embrapa Unidades Centrais. |
| |
10. | | MAIA, A. de H. N.; SOUSA, K. K. A.; SILVA, R. B. Q. da; VIVIAN, R. Modelo de Cox com preditores qualitativos para análise de padrões temporais de mudança de fase em insetos. In: SIMPÓSIO NACIONAL DE PROBABILIDADE E ESTATÍSTICA, 23., 2018, São Pedro, SP. [Resumos...] São Pedro, SP: ABE, 2018. Biblioteca(s): Embrapa Meio Ambiente; Embrapa Meio-Norte. |
| |
14. | | FERNANDES, D. R. R.; SILVA, R. B. Q. da; HAMADA, N. Order Hymenoptera. In: HAMADA, N.; THORP, J. H.; ROGERS, D. C. (Ed.). Keys to Neotropical Hexapoda. Thorp and covich's freshwater invertebrates. 4 Ed. Londres: Academic Press, Elsevier, 2018, v. 3, p. 339-347. Autoria na publicação: Ranyse B. Querino. Biblioteca(s): Embrapa Meio-Norte. |
| |
18. | | NAVIA, D.; SILVA, R. B. Q. da; NERY, R. de S.; VIVIAN, R.; FERRAGUT, F. Phytophagous and predatory mites in the soybean? cowpea succession cropping system in Brazil? associations could promote sustainability? Bulletin OILB-SROP, v. 120, p. 38-42, 2016. Edição dos Proceedings of the Fifth Working Group Meeting IOBC-WPRS, OILB-SROP, Castello de la Plana (Spain), 2015. Biblioteca(s): Embrapa Meio-Norte. |
| |
19. | | NAVIA, D.; SILVA, R. B. Q. da; NERY, R. de S.; VIVIAN, R.; FERRAGUT, F. Phytophagous and predatory mites in the soybean ? cowpea succession cropping system in Brazil ? associations could promote sustainability? Bulletin OILB-SROP, v. 120, p. 38-42, 2016. Edição dos Proceedings of the Fifth Working Group Meeting IOBC-WPRS, OILB-SROP, Castello de la Plana (Spain), 2015. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
Registros recuperados : 65 | |
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
11/07/2018 |
Data da última atualização: |
11/07/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
RAMALHO, S. R.; BEZERRA, C. da S.; LIMA, L. S.; MARIA NETO, S.; OLIVEIRA, C. F. R. de; VERBISCK, N. V.; MACEDO, M. L. R. |
Afiliação: |
Suellen Rodrigues Ramalho; Cezar da Silva Bezerra; Letícia Souza Lima; Simone Maria Neto; Caio Fernando Ramalho de Oliveira; NEWTON VALERIO VERBISCK, CNPGC; Maria Lígia Rodrigues Macedo. |
Título: |
Novel peptidase kunitz Inhibitor from platypodium elegans seeds is active against Spodoptera frugiperda larvae. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Journal Agricultural Food Chemistry. v. 66, n. 6, p. 1349-1358, fev. 2018. |
Idioma: |
Português |
Conteúdo: |
A novel Kunitz-type inhibitor from Platypodium elegans seeds (PeTI) was purified and characterized. The mass spectrometry analyses of PeTI indicated an intact mass of 19?701 Da and a partial sequence homologous to Kunitz inhibitors. PeTI was purified by ion exchange and affinity chromatographies. A complex with a 1:1 ratio was obtained only for bovine trypsin, showing a Ki = 0.16 nM. Stability studies showed that PeTI was stable over a wide range of temperature (37-80 °C) and pH (2-10). The inhibitory activity of PeTI was affected by dithiothreitol (DTT). Bioassays of PeTI on Spodoptera frugiperda showed negative effects on larval development and weight gain, besides extending the insect life cycle. The activities of digestive enzymes, trypsin and chymotrypsin, were reduced by feeding larvae with 0.2% PeTI in an artificial diet. In summary, we describe a novel Kunitz inhibitor with promising biotechnological potential for pest control. |
Palavras-Chave: |
Kunitz-type inhibitor; Pest insect. |
Thesaurus NAL: |
Fabaceae; Trypsin inhibitors. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01706naa a2200241 a 4500 001 2093186 005 2018-07-11 008 2018 bl uuuu u00u1 u #d 100 1 $aRAMALHO, S. R. 245 $aNovel peptidase kunitz Inhibitor from platypodium elegans seeds is active against Spodoptera frugiperda larvae.$h[electronic resource] 260 $c2018 520 $aA novel Kunitz-type inhibitor from Platypodium elegans seeds (PeTI) was purified and characterized. The mass spectrometry analyses of PeTI indicated an intact mass of 19?701 Da and a partial sequence homologous to Kunitz inhibitors. PeTI was purified by ion exchange and affinity chromatographies. A complex with a 1:1 ratio was obtained only for bovine trypsin, showing a Ki = 0.16 nM. Stability studies showed that PeTI was stable over a wide range of temperature (37-80 °C) and pH (2-10). The inhibitory activity of PeTI was affected by dithiothreitol (DTT). Bioassays of PeTI on Spodoptera frugiperda showed negative effects on larval development and weight gain, besides extending the insect life cycle. The activities of digestive enzymes, trypsin and chymotrypsin, were reduced by feeding larvae with 0.2% PeTI in an artificial diet. In summary, we describe a novel Kunitz inhibitor with promising biotechnological potential for pest control. 650 $aFabaceae 650 $aTrypsin inhibitors 653 $aKunitz-type inhibitor 653 $aPest insect 700 1 $aBEZERRA, C. da S. 700 1 $aLIMA, L. S. 700 1 $aMARIA NETO, S. 700 1 $aOLIVEIRA, C. F. R. de 700 1 $aVERBISCK, N. V. 700 1 $aMACEDO, M. L. R. 773 $tJournal Agricultural Food Chemistry.$gv. 66, n. 6, p. 1349-1358, fev. 2018.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|