01706naa a2200241 a 450000100080000000500110000800800410001910000190006024501390007926000090021852009550022765000130118265000230119565300260121865300160124470000220126070000160128270000190129870000260131770000200134370000210136377300800138420931862018-07-11       2018    bl uuuu     u00u1 u    #d1 aRAMALHO, S. R.  aNovel peptidase kunitz Inhibitor from platypodium elegans seeds is active against Spodoptera frugiperda larvae.h[electronic resource]  c2018  aA novel Kunitz-type inhibitor from Platypodium elegans seeds (PeTI) was purified and characterized. The mass spectrometry analyses of PeTI indicated an intact mass of 19?701 Da and a partial sequence homologous to Kunitz inhibitors. PeTI was purified by ion exchange and affinity chromatographies. A complex with a 1:1 ratio was obtained only for bovine trypsin, showing a Ki = 0.16 nM. Stability studies showed that PeTI was stable over a wide range of temperature (37-80 °C) and pH (2-10). The inhibitory activity of PeTI was affected by dithiothreitol (DTT). Bioassays of PeTI on Spodoptera frugiperda showed negative effects on larval development and weight gain, besides extending the insect life cycle. The activities of digestive enzymes, trypsin and chymotrypsin, were reduced by feeding larvae with 0.2% PeTI in an artificial diet. In summary, we describe a novel Kunitz inhibitor with promising biotechnological potential for pest control.  aFabaceae  aTrypsin inhibitors  aKunitz-type inhibitor  aPest insect1 aBEZERRA, C. da S.1 aLIMA, L. S.1 aMARIA NETO, S.1 aOLIVEIRA, C. F. R. de1 aVERBISCK, N. V.1 aMACEDO, M. L. R.  tJournal Agricultural Food Chemistry.gv. 66, n. 6, p. 1349-1358, fev. 2018.