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| Registros recuperados : 70 | |
| 8. |  | ALBUQUERQUE, T. C. S. de; LIMA, H. E. de; LIMA, A. O.; SILVA, L. M.; SILVA, P. L. Absorção de nitrogênio em acessos de cupuaçuzeiro com diferentes níveis de tolerância a vassoura-de-bruxa em Roraima. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 25., 2017; REUNIÃO ANUAL DA SOCIEDADE INTERAMERICANA DE HORTICULTURA TROPICAL, 62., 2017. Resumos... Porto Seguro, BA: SIHT, 2017.| Biblioteca(s): Embrapa Roraima. |
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| 9. | | LIMA, A. O. de S.; SCHWARTZ, G.; SILVA, A. R.; LUNZ, A. M.; MELLO, A. H. de; ALBINO, U. B. Adaptabilidade de clones de eucalipto à inoculação de fungos micorrízicos em solo arenoso em São Domingos do Araguaia - PA. Colloquium Agrariae, v. 15, n. 6, p. 1-10, nov./dez. 2019.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | MENDES, R.; KMIT, M. C. P.; LIMA, A. O. S.; FREITAS, R. C.; ROMAGNOLI, E. M.; ABDALLA, A. L. Metagenomic analysis of sheep rumen microbiome for carbohidrate-active genes discovery. In: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 16., 2016, Montreal. Proceedings... Wageningen: The International Society for Microbial Ecology (ISME), 2016. p. 836.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 11. | | LIMA, A. O. de S.; SCHWARTZ, G.; SILVA, A. R.; LUNZ, A. M.; MELLO, A. H. de; ALBINO, U. B. Efeito de microrganismos indutores de crescimento e adubação química em paricá em solo arenoso. Brazilian Journal of Development, v. 6, n. 11, p. 91670-91683, nov. 2020.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | KMIT, M. C. P.; LIMA, A. O. S.; FREITAS, R. C.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R. Exploring the sheep rumen shotgun sequencing for funcional analysis and lignocellulolitic enzyme discovery. In: CONGRESO LATINOAMERICANO DE MICROBIOLOGÍA, 23.; CONGRESO ARGENTINO DE MICROBIOLOGIA, 14.; 2016, Rosario. Libro de Resumenes... Rosario: Asociación Latinoamericana de Microbiología; Asociación Argentina de Microbiología, 2016. Ref. JU-1377.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 13. | | OLIVEIRA, K. S. de; LIMA, A. O. de; AFONSO, J.; MELO, A. L. de; REGITANO, L. C. de A. Variantes da região promotora do gene KCNJ11 em bovinos da raça Nelore. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 10., 2018, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste, 2018. p. 26. (Embrapa Instrumentação. Documentos, 68). Editores técnicos: Daniel Souza Corrêa, Elaine Cristina Paris, Maria Alice Martins, Paulino Ribeiro Villas Boas, Wilson Tadeu Lopes da Silva.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 14. | | MOKRY, F. B.; LIMA, A. O. de; MUDADU, M. A.; HIGA, R. H.; MEIRELLES, S. L. C.; REGITANO, L. C. de A. An insight into the linkage disequilibrium map of the Canchim beef cattle breed. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 8., 2012, Campinas. Abstract book... Ribeirão Preto: AB3C, 2012. Não paginado. X-MEETING 2012.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 15. | | OLIVEIRA, K. S. de; LIMA, A. O. de; AFONSO, J.; CARDOSO, T. F.; BRUSCADIN, J. J.; REGITANO, L. C. de A. Associação entre variantes cis regulatórias do gene KCNJ11e características econômicas em bovinos Nelore. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 11., 2019, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação, 2019. p.23| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 16. | | MOKRY, F. B.; LIMA, A. O. de; URBINATI, I.; TORRES JUNIOR, R. A. de A.; HIGA, R. H.; REGITANO, L. C. de A. Associação de SNPs com características de carcaça em uma população da raça Canchim. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 9., 2012, João Pessoa. Anais... João Pessoa: SBMA, 2012. Não paginado. SBMA 2012.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Gado de Corte; Embrapa Pecuária Sudeste. |
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| 17. | | SERRA, V.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; LIMA, A. O. de; GASPARIN, G.; COUTINHO, L. L.; MOURÃO, G. B.; REGITANO, L. C. de A. Análise de qualidade de RNA para estudo do perfil transcriptômico de animais extremos para eficiência alimentar da raça Nelore. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 5., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste: Embrapa Instrumentação , 2013. p. 12 (Embrapa Pecuária Sudeste. Documentos, 110). Editado por Ana Rita de Araújo nogueira, simone Cristina Méo Nicura| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 18. | | LOPES, L. D.; SILVA, L. R. F.; ROMAGNOLI, E. M.; FERREIRA, C; TAKETANI, R. G.; LOUVANDINI, H; LIMA, A. O. S.; ABDALLA, A. L.; MENDES, R. Sheep rumen microbiome sequencing using Ion Torrent (PGM) platform. In: SYMPOSIUM ON BACTERIAL GENETICS AND ECOLOGY, 12., 2013, Ljubljana (Slovenia). Networking and plasticity of microbial communities: the secret to success. Ljubljana: University of Ljubljana. 2013. p. 68, ref. P31.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 19. | | BORTOLON, L.; LIMA, A. O.; BORTOLON, E. S. O.; ROCHA, P. H. F.; CONCEIÇÃO, W. S. S.; SOUZA, J. P.; ALMEIDA, R. E. M.; CAMARGO, F. P. Spatial and temporal variation of near surface soil moisture and soil temperature in integrated crop-livestock system. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FOREST SYSTEMS; INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 3., 2015, Brasília, DF. Towards sustainable intensification: proceedings. Brasília, DF: Embrapa, 2015.| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 20. | | SOARES JUNIOR, F. L.; DIAS, A. C. F.; FASANELLA, C. C.; TAKETANI, R. G.; LIMA, A. O. S.; MELO, I. S. de; ANDREOTE, F. D. Endo- and exoglucanase activities in bacteria from mangrove sediment. Brazilian Journal of Microbiology, Piracicaba, v. 44, n. 3, p. 969-976, 2013.| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 70 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
04/01/2016 |
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Data da última atualização: |
10/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
LOPES, L. D.; LIMA, A. O. S.; TAKETANI, R. G.; DARIAS, P.; SILVA, L. R. F.; ROMAGNOLI, E. M.; LOUVANDINI, H.; ABDALLA, A. L.; MENDES, R. |
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Afiliação: |
LUCAS DANTAS LOPES; ANDRE OLIVEIRA DE SOUZA LIMA, UNIVALI; RODRIGO GOUVEA TAKETANI; PHILLIP DARIAS, UNIVALI; LILIA RAQUEL FE DA SILVA, UFPI; EMILIANA MANESCO ROMAGNOLI; HELDER LOUVANDINI, CENA-USP; ADIBE LUIZ ABDALLA, CENA-USP; RODRIGO MENDES, CNPMA. |
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Título: |
Exploring the sheep rumen microbiome for carbohydrate-active enzymes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Antonie van Leeuwenhoek, Amsterdam, v. 108, n. 1, p. 15-30, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) representing 12 glycosyl hydrolase families (Pfam database). Overall, we identified a total of 28 lignocellulases, 22 amylases and 9 other putative CAE, showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes MenosAbstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) represen... Mostrar Tudo |
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Palavras-Chave: |
Ion torrent (PGM). |
|
Thesagro: |
Amilase; Bactéria; Enzima; Ovelha; Rúmen. |
|
Thesaurus NAL: |
Amylases; Bacterial communities; Lignocellulose; Metagenomics; Phylogeny. |
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Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 02652naa a2200349 a 4500
001 2032734
005 2016-03-10
008 2015 bl uuuu u00u1 u #d
100 1 $aLOPES, L. D.
245 $aExploring the sheep rumen microbiome for carbohydrate-active enzymes.$h[electronic resource]
260 $c2015
520 $aAbstract: The rumen is a complex ecosystem enriched for microorganisms able to degrade biomass during the animal?s digestion process. The recovery of new enzymes from naturally evolved biomass-degrading microbial communities is a promising strategy to overcome the inefficient enzymatic plant destruction in industrial production of biofuels. In this context, this study aimed to describe the bacterial composition and functions in the sheep rumen microbiome, focusing on carbohydrate-active enzymes (CAE). Here, we used phylogenetic profiling analysis (inventory of 16S rRNA genes) combined with metagenomics to access the rumen microbiome of four sheep and explore its potential to identify fibrolytic enzymes. The bacterial community was dominated by Bacteroidetes and Firmicutes, followed by Proteobacteria. As observed for other ruminants, Prevotella was the dominant genus in the microbiome, comprising more than 30 % of the total bacterial community. Multivariate analysis of the phylogenetic profiling data and chemical parameters showed a positive correlation between the abundance of Prevotellaceae (Bacteroidetes phylum) and organic matter degradability. A negative correlation was observed between Succinivibrionaceae (Proteobacteria phylum) and methane production. An average of 2 % of the shotgun metagenomic reads was assigned to putative CAE when considering nine protein databases. In addition, assembled contigs allowed recognition of 67 putative partial CAE (NCBI-Refseq) representing 12 glycosyl hydrolase families (Pfam database). Overall, we identified a total of 28 lignocellulases, 22 amylases and 9 other putative CAE, showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes
650 $aAmylases
650 $aBacterial communities
650 $aLignocellulose
650 $aMetagenomics
650 $aPhylogeny
650 $aAmilase
650 $aBactéria
650 $aEnzima
650 $aOvelha
650 $aRúmen
653 $aIon torrent (PGM)
700 1 $aLIMA, A. O. S.
700 1 $aTAKETANI, R. G.
700 1 $aDARIAS, P.
700 1 $aSILVA, L. R. F.
700 1 $aROMAGNOLI, E. M.
700 1 $aLOUVANDINI, H.
700 1 $aABDALLA, A. L.
700 1 $aMENDES, R.
773 $tAntonie van Leeuwenhoek, Amsterdam$gv. 108, n. 1, p. 15-30, 2015.
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