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| Registros recuperados : 4 | |
| 1. |  | DIE, J. V.; ROMAN, B.; SALVADOR, N.; RODRIGUEZ, M. A. D.; GONZÁLEZ-VERDEJO, C. I. Expression analysis of Pisum sativum putative defence genes during Orobanche crenata infection. Crop and Pasture Science, Victoria, v. 60, n. 5, p. 490-498, mai. 2009.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 2. | | DIE, J. V.; GONZÁLEZ VERDEJO, C. I.; DITA, M. A.; SALVADOR, N.; ROMAN, B. Gene expression analysis of molecular mechanisms of defense induced in Medicago truncatula parasitized by Orobanche crenata. Plant Physiology and Biochemistry, Paris, v. 47, n. 3, p. 635-641, mar. 2009.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 3. | | GONZÁLEZ-VERDEJO, C. I.; DITA, M. A.; NADAL, S.; MORENO, M. T.; ROMÁN, B. Sucrose application suppresses infection of the parasitic plant Orobanche ramosa en tomate (Lycopersicon esculentum). Agrociência, México, v. 42, n. 5, p. 513-517, ago. 2008. Texto em inglês e espanhol.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 4. | | DIE, J. V.; DITA, M. A.; KRAJINSKI, F.; GONZÁLEZ-VERDEJO, C. I.; RUBIALES, D.; MORENO, M. T.; ROMÁN, B. Identification by suppression subtractive hybridization and expression analysis of Medicago truncatula putative defence genes in response to Orobanche crenata parasitization. Physiological and Molecular Plant Pathology, v. 70, p. 49-59, 2007.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 4 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/07/2009 |
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Data da última atualização: |
22/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
DIE, J. V.; ROMAN, B.; SALVADOR, N.; RODRIGUEZ, M. A. D.; GONZÁLEZ-VERDEJO, C. I. |
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Afiliação: |
José Vicente Die, IFAPA; Belén Román, IFAPA; Nadal Moyano Salvador, IFAPA; MIGUEL ANGEL DITA RODRIGUEZ, CNPMF; Clara Isabel González-Verdejo, IFAPA. |
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Título: |
Expression analysis of Pisum sativum putative defence genes during Orobanche crenata infection. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Crop and Pasture Science, Victoria, v. 60, n. 5, p. 490-498, mai. 2009. |
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ISSN: |
1836-0947 |
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DOI: |
DOI: 10.1071/CP08274 |
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Idioma: |
Inglês |
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Conteúdo: |
The root holoparasitic angiosperm Orobanche crenata is a severe constraint to the cultivation of legumes. Breeding for resistance is a difficult task. Understanding the mechanisms underlying host resistance is a fundamental issue for the genetic improvement of legumes. In this work, the temporal expression patterns of 8 defence-genes known to be involved in different metabolic pathways activated during several plant-pathogen interactions were investigated in Pisum sativum. Molecular analyses were carried out using quantitative real-time polymerase chain reaction during the initial stages of the parasitisation process in susceptible (Messire) and incompletely resistant (Ps624) pea genotypes. Transcriptional changes in response to O. crenata revealed induction of genes putatively encoding pathogenesis-related proteins, peroxidase activity, and dehydration stress-responsive signalling. This, combined with high constitutive gene expression mediating the phenylpropanoid pathway were observed as part of the defence mechanisms triggered in Ps624 to restrict the growth of the parasite. |
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Palavras-Chave: |
Plant defence; Real-time PCR. |
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Thesagro: |
Melhoramento Genético Vegetal; Patógeno. |
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Thesaurus NAL: |
parasitic plants. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/655752/1/Expression-analysis-of-Pisum-sativum-putative-defence-genes-during-Orobanche-crenata-infect.pdf
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Marc: |
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100 1 $aDIE, J. V.
245 $aExpression analysis of Pisum sativum putative defence genes during Orobanche crenata infection.$h[electronic resource]
260 $c2009
520 $aThe root holoparasitic angiosperm Orobanche crenata is a severe constraint to the cultivation of legumes. Breeding for resistance is a difficult task. Understanding the mechanisms underlying host resistance is a fundamental issue for the genetic improvement of legumes. In this work, the temporal expression patterns of 8 defence-genes known to be involved in different metabolic pathways activated during several plant-pathogen interactions were investigated in Pisum sativum. Molecular analyses were carried out using quantitative real-time polymerase chain reaction during the initial stages of the parasitisation process in susceptible (Messire) and incompletely resistant (Ps624) pea genotypes. Transcriptional changes in response to O. crenata revealed induction of genes putatively encoding pathogenesis-related proteins, peroxidase activity, and dehydration stress-responsive signalling. This, combined with high constitutive gene expression mediating the phenylpropanoid pathway were observed as part of the defence mechanisms triggered in Ps624 to restrict the growth of the parasite.
650 $aparasitic plants
650 $aMelhoramento Genético Vegetal
650 $aPatógeno
653 $aPlant defence
653 $aReal-time PCR
700 1 $aROMAN, B.
700 1 $aSALVADOR, N.
700 1 $aRODRIGUEZ, M. A. D.
700 1 $aGONZÁLEZ-VERDEJO, C. I.
773 $tCrop and Pasture Science, Victoria$gv. 60, n. 5, p. 490-498, mai. 2009.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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