01870naa a2200253 a 450000100080000000500110000800800410001902200140006002400300007410000150010424501230011926000090024252010990025165000210135065000350137165000140140665300180142065300180143870000140145670000170147070000240148770000290151177300760154016557522022-08-22       2009    bl uuuu     u00u1 u    #d  a1836-09477 aDOI: 10.1071/CP082742DOI1 aDIE, J. V.  aExpression analysis of Pisum sativum putative defence genes during Orobanche crenata infection.h[electronic resource]  c2009  aThe root holoparasitic angiosperm Orobanche crenata is a severe constraint to the cultivation of legumes. Breeding for resistance is a difficult task. Understanding the mechanisms underlying host resistance is a fundamental issue for the genetic improvement of legumes. In this work, the temporal expression patterns of 8 defence-genes known to be involved in different metabolic pathways activated during several plant-pathogen interactions were investigated in Pisum sativum. Molecular analyses were carried out using quantitative real-time polymerase chain reaction during the initial stages of the parasitisation process in susceptible (Messire) and incompletely resistant (Ps624) pea genotypes. Transcriptional changes in response to O. crenata revealed induction of genes putatively encoding pathogenesis-related proteins, peroxidase activity, and dehydration stress-responsive signalling. This, combined with high constitutive gene expression mediating the phenylpropanoid pathway were observed as part of the defence mechanisms triggered in Ps624 to restrict the growth of the parasite.  aparasitic plants  aMelhoramento Genético Vegetal  aPatógeno  aPlant defence  aReal-time PCR1 aROMAN, B.1 aSALVADOR, N.1 aRODRIGUEZ, M. A. D.1 aGONZÁLEZ-VERDEJO, C. I.  tCrop and Pasture Science, Victoriagv. 60, n. 5, p. 490-498, mai. 2009.