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1. | | VASCONCELLOS, V. M.; TARDIOLI, P. W.; GIORDANO, R. L. C.; FARINAS, C. S. Addition of metal ions to a (hemi)cellulolytic enzymatic cocktail produced in-house improves its activity, thermostability, and efficiency in the saccharification of pretreated sugarcane bagasse. New biotechnology, [S. l.], v. 33, n. 3, p. 331-337, 2016. Biblioteca(s): Embrapa Instrumentação. |
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7. | | VASCONCELLOS, V. M.; FLORENCIO, C.; BADINO, A. C.; GIORDANO, R. L. C.; TARDIOLI, P. W.; FARINAS, C. S. Estudo comparativo da termoestabilidade de enzimas produzidas por fungos filamentosos em cultivo submerso e combinado. In: CONGRESSO BRASILEIRO DE ENGENHARIA QUÍMICA - COBEQ, 20.; ENCONTRO BRASILEIRO SOBRE O ENSINO DE ENGENHARIA QUÍMICA, 15., 2014, Florianópolis. Anais... Florianópolis: UFSC, 2014. Não paginado. Biblioteca(s): Embrapa Instrumentação. |
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9. | | CARVALHO, M. L.; ZUÑIGA, U. F. R.; SUAREZ, C. A. G.; GIORDANO, R. C.; GIORDANO, R. L. C.; RODRIGUES, D. de S.; JUNIOR, R. S. Kinetic study of the enzymatic hydrolysis of sugarcane bagasse. Brazilian Journal of Chemical Engineering, v. 30, n. 3, p. 437-447, 2013. Biblioteca(s): Embrapa Agroenergia. |
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10. | | RODRÍGUEZ-ZÚÑIGA, U. F.; FARINAS, C. S.; CARNEIRO, R. L. SILVA, G. M. CRUZ, A. J. G. GIORDANO, R. L. C. Giordano, R. C. RIBEIRO, M. P. A. Fast determination of the composition of pretreated sugarcane bagasse using near-infrared spectroscopy. BioEnergy Research, [S, l.], v. 1, p. 1, 2014. Biblioteca(s): Embrapa Instrumentação. |
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11. | | RODRÍGUEZ-ZÚÑIGA, U. F.; SILVA, G. M.; NASCIMENTO, V. M.; WOLF, L. D.; SILVA, V. F.; CRUZ, A. J. G.; GIORDANO, R. L. C.; ROCHA, G. J.; CARNEIRO, R. L.; FARINAS, C. S.; GIORDANO, R. C. Compositional study by NIR spectroscopy of sugarcane bagasse subjected to different pretreatments for ethanol production. In: CONGRESSO BRASILEIRO DE ENGENHARIA QUÍMICA, 19., 2012, Búzios. Anais... São Paulo: ABEQ, p. 7635-7643, 2012. Biblioteca(s): Embrapa Instrumentação. |
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12. | | RODRIGUES-ZUÑIGA, U. F.; NASCIMENTO, V. M.; WOLF, L. D.; SILVA, G, M.; CRUZ, A. J. G.; GIORDANO, R. L. C., CARNEIRO, R. L.; SILVA, W. T. L. da; FARINAS, C. S.; GIORDANO, R. C. Role of chemometrics for at-field application of NIR spectroscopy to predict sugarcane enzymatic hydrolysis performance. In: BRAZILIAN BIOENERGY SCIENCE AND TECHNOLOGY CONFERENCE - BBEST, 1., 2011, Campos do Jordão. [Abstracts...]. São Paulo: FAPESP, 2011. 1 CD-ROM. Biblioteca(s): Embrapa Instrumentação. |
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Registros recuperados : 12 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
04/10/2013 |
Data da última atualização: |
21/09/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
CARVALHO, M. L.; ZUÑIGA, U. F. R.; SUAREZ, C. A. G.; GIORDANO, R. C.; GIORDANO, R. L. C.; RODRIGUES, D. de S.; JUNIOR, R. S. |
Afiliação: |
M. L. Carvalho, Universidade Federal de São Carlos; U. F. Rodríguez-Zúñiga, Universidade Federal de São Carlos; C. A. G. Suarez, Universidade Federal de São Carlos; R. C. Giordano, Universidade Federal de São Carlos; R. L. C. Giordano, Universidade Federal de São Carlos; DASCIANA DE SOUSA RODRIGUES, CNPAE; R. Sousa Jr., Universidade Federal de São Carlos. |
Título: |
Kinetic study of the enzymatic hydrolysis of sugarcane bagasse. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Brazilian Journal of Chemical Engineering, v. 30, n. 3, p. 437-447, 2013. |
DOI: |
10.1590/S0104-66322013000300002 |
Idioma: |
Inglês |
Conteúdo: |
This work presents a kinetic study of the enzymatic hydrolysis of three cellulosic substrates: filter paper (FP), used as a low recalcitrance substrate model; steam exploded sugarcane bagasse (SB); and weak acid pretreated SB (1:20 dry bagasse:H2SO4 solution 1% w/w), the last two delignified with 4% NaOH (w/w). The influence of substrate concentration was assessed in hydrolysis experiments in a shaker, using Accellerase® 1500, at pH 4.8, in 50 mM sodium citrate buffer. Cellulose loads (weightsubstrate/weighttotal) were changed between 0.5%-13% (for FP) and 0.99%-9.09% (for SB). For FP and low loads of steam exploded SB, it was possible to fit pseudo-homogeneous Michaelis-Menten models (with inhibition). For FP and higher loads of steam exploded SB, modified Michaelis-Menten models were fitted. Besides, it was observed that, after retuning of the model parameters, it is possible to apply a model fitted for one situation to a different case. Chrastil models were also fitted and they were the only feasible approach for the highly recalcitrant acid-treated SB. |
Palavras-Chave: |
Cellulose enzymatic hydrolysis; Kinetic study. |
Thesaurus NAL: |
sugarcane bagasse. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01822naa a2200241 a 4500 001 1967824 005 2017-09-21 008 2013 bl uuuu u00u1 u #d 024 7 $a10.1590/S0104-66322013000300002$2DOI 100 1 $aCARVALHO, M. L. 245 $aKinetic study of the enzymatic hydrolysis of sugarcane bagasse.$h[electronic resource] 260 $c2013 520 $aThis work presents a kinetic study of the enzymatic hydrolysis of three cellulosic substrates: filter paper (FP), used as a low recalcitrance substrate model; steam exploded sugarcane bagasse (SB); and weak acid pretreated SB (1:20 dry bagasse:H2SO4 solution 1% w/w), the last two delignified with 4% NaOH (w/w). The influence of substrate concentration was assessed in hydrolysis experiments in a shaker, using Accellerase® 1500, at pH 4.8, in 50 mM sodium citrate buffer. Cellulose loads (weightsubstrate/weighttotal) were changed between 0.5%-13% (for FP) and 0.99%-9.09% (for SB). For FP and low loads of steam exploded SB, it was possible to fit pseudo-homogeneous Michaelis-Menten models (with inhibition). For FP and higher loads of steam exploded SB, modified Michaelis-Menten models were fitted. Besides, it was observed that, after retuning of the model parameters, it is possible to apply a model fitted for one situation to a different case. Chrastil models were also fitted and they were the only feasible approach for the highly recalcitrant acid-treated SB. 650 $asugarcane bagasse 653 $aCellulose enzymatic hydrolysis 653 $aKinetic study 700 1 $aZUÑIGA, U. F. R. 700 1 $aSUAREZ, C. A. G. 700 1 $aGIORDANO, R. C. 700 1 $aGIORDANO, R. L. C. 700 1 $aRODRIGUES, D. de S. 700 1 $aJUNIOR, R. S. 773 $tBrazilian Journal of Chemical Engineering$gv. 30, n. 3, p. 437-447, 2013.
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