01822naa a2200241 a 450000100080000000500110000800800410001902400410006010000200010124500910012126000090021252010780022165000220129965300350132165300180135670000220137470000210139670000200141770000230143770000240146070000180148477300780150219678242017-09-21       2013    bl uuuu     u00u1 u    #d7 a10.1590/S0104-663220130003000022DOI1 aCARVALHO, M. L.  aKinetic study of the enzymatic hydrolysis of sugarcane bagasse.h[electronic resource]  c2013  aThis work presents a kinetic study of the enzymatic hydrolysis of three cellulosic substrates: filter paper (FP), used as a low recalcitrance substrate model; steam exploded sugarcane bagasse (SB); and weak acid pretreated SB (1:20 dry bagasse:H2SO4 solution 1% w/w), the last two delignified with 4% NaOH (w/w). The influence of substrate concentration was assessed in hydrolysis experiments in a shaker, using Accellerase® 1500, at pH 4.8, in 50 mM sodium citrate buffer. Cellulose loads (weightsubstrate/weighttotal) were changed between 0.5%-13% (for FP) and 0.99%-9.09% (for SB). For FP and low loads of steam exploded SB, it was possible to fit pseudo-homogeneous Michaelis-Menten models (with inhibition). For FP and higher loads of steam exploded SB, modified Michaelis-Menten models were fitted. Besides, it was observed that, after retuning of the model parameters, it is possible to apply a model fitted for one situation to a different case. Chrastil models were also fitted and they were the only feasible approach for the highly recalcitrant acid-treated SB.  asugarcane bagasse  aCellulose enzymatic hydrolysis  aKinetic study1 aZUÑIGA, U. F. R.1 aSUAREZ, C. A. G.1 aGIORDANO, R. C.1 aGIORDANO, R. L. C.1 aRODRIGUES, D. de S.1 aJUNIOR, R. S.  tBrazilian Journal of Chemical Engineeringgv. 30, n. 3, p. 437-447, 2013.