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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/05/2010 |
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Data da última atualização: |
02/08/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OCHOA-CORONA, F. M.; LOCALI-FABRIS, E. C.; FREITAS-ASTÚA, J. |
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Afiliação: |
Oklahoma University; Eliane Cristina Locali-Fabris, APTA; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
An RT-PCR procedure for detection and surveillance of Citrus leprosis virus C (CiLV-C) in post-entry quarantine stocks of citrus. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Phytopathology, St. Paul, v. 99, n. 6, S28, 2009. Suplement |
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Idioma: |
Inglês |
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Notas: |
Abstracts of presentations, Annual Meeting of the APS 2009. p. S28. |
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Conteúdo: |
Citrus leprosis virus C (CiLV-C), which is transmitted both mechanically and by the mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae), causes significant disease damage in South and Central America. Citrus leprosis disease was first recorded in Florida in 1925, but is believed eradicated through improved mite control procedures. CiLV-C is a threat to citrus producing nations where it is not present, such as New Zealand and the USA, and a sensitive detection method is required for screening and biosecurity of suspect quarantine material. CiLV-C is mechanically transmitted, posses a bipartite RNA genome and was believed to be a rhabdovirus. After being sequenced, CiLV-C was proposed as the type member of a new genus, Cilevirus, related to several (+) ssRNA viruses. Of two known morphological types of CiLV particles, the cytoplasmic type (CiLV-C) is prevalent than the nuclear type (CiLV-N) in Brazil and elsewhere. A pair of diagnostic primers, amplifying a segment of 278 bp located at the RNA-2 p15 gene of CiLV-C was designed using the Web software pathway Primer3-mFOLD-BLASTn. A thermodynamically robust RT-PCR that performs well in a range of melting temperatures and specifically optimized for CiLV-C was developed, and is a feasible tool to be used in quarantine. |
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Thesagro: |
Brevipalpus Phoenicis; Fruta Cítrica. |
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Thesaurus NAL: |
Citrus. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/748515/1/An-RT-PCR-procedure-for-detection-and-surveillance-of-Citrus-leprosis-virus-C-CiLV-C-in-post-entry-quarantine-stocks-of-citrus.pdf
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Marc: |
LEADER 01966nam a2200181 a 4500
001 1748515
005 2023-08-02
008 2009 bl uuuu u00u1 u #d
100 1 $aOCHOA-CORONA, F. M.
245 $aAn RT-PCR procedure for detection and surveillance of Citrus leprosis virus C (CiLV-C) in post-entry quarantine stocks of citrus.$h[electronic resource]
260 $aPhytopathology, St. Paul, v. 99, n. 6, S28, 2009. Suplement$c2009
500 $aAbstracts of presentations, Annual Meeting of the APS 2009. p. S28.
520 $aCitrus leprosis virus C (CiLV-C), which is transmitted both mechanically and by the mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae), causes significant disease damage in South and Central America. Citrus leprosis disease was first recorded in Florida in 1925, but is believed eradicated through improved mite control procedures. CiLV-C is a threat to citrus producing nations where it is not present, such as New Zealand and the USA, and a sensitive detection method is required for screening and biosecurity of suspect quarantine material. CiLV-C is mechanically transmitted, posses a bipartite RNA genome and was believed to be a rhabdovirus. After being sequenced, CiLV-C was proposed as the type member of a new genus, Cilevirus, related to several (+) ssRNA viruses. Of two known morphological types of CiLV particles, the cytoplasmic type (CiLV-C) is prevalent than the nuclear type (CiLV-N) in Brazil and elsewhere. A pair of diagnostic primers, amplifying a segment of 278 bp located at the RNA-2 p15 gene of CiLV-C was designed using the Web software pathway Primer3-mFOLD-BLASTn. A thermodynamically robust RT-PCR that performs well in a range of melting temperatures and specifically optimized for CiLV-C was developed, and is a feasible tool to be used in quarantine.
650 $aCitrus
650 $aBrevipalpus Phoenicis
650 $aFruta Cítrica
700 1 $aLOCALI-FABRIS, E. C.
700 1 $aFREITAS-ASTÚA, J.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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