01966nam a2200181 a 450000100080000000500110000800800410001910000240006024501570008426000700024150000720031152012970038365000110168065000260169165000190171770000250173670000230176117485152023-08-02       2009    bl uuuu     u00u1 u    #d1 aOCHOA-CORONA, F. M.  aAn RT-PCR procedure for detection and surveillance of Citrus leprosis virus C (CiLV-C) in post-entry quarantine stocks of citrus.h[electronic resource]  aPhytopathology, St. Paul, v. 99, n. 6, S28, 2009. Suplementc2009  aAbstracts of presentations, Annual Meeting of the APS 2009. p. S28.  aCitrus leprosis virus C (CiLV-C), which is transmitted both mechanically and by the mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae), causes significant disease damage in South and Central America. Citrus leprosis disease was first recorded in Florida in 1925, but is believed eradicated through improved mite control procedures. CiLV-C is a threat to citrus producing nations where it is not present, such as New Zealand and the USA, and a sensitive detection method is required for screening and biosecurity of suspect quarantine material. CiLV-C is mechanically transmitted, posses a bipartite RNA genome and was believed to be a rhabdovirus. After being sequenced, CiLV-C was proposed as the type member of a new genus, Cilevirus, related to several (+) ssRNA viruses. Of two known morphological types of CiLV particles, the cytoplasmic type (CiLV-C) is prevalent than the nuclear type (CiLV-N) in Brazil and elsewhere. A pair of diagnostic primers, amplifying a segment of 278 bp located at the RNA-2 p15 gene of CiLV-C was designed using the Web software pathway Primer3-mFOLD-BLASTn. A thermodynamically robust RT-PCR that performs well in a range of melting temperatures and specifically optimized for CiLV-C was developed, and is a feasible tool to be used in quarantine.  aCitrus  aBrevipalpus Phoenicis  aFruta Cítrica1 aLOCALI-FABRIS, E. C.1 aFREITAS-ASTÚA, J.