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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
11/07/2013 |
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Data da última atualização: |
08/05/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BATISTA, P. F.; LIMA, M. A. C. de; LEAO, P. C. de S.; ROSATTI, S. R.; SANTOS, A. C. B. dos; ALVES, R. E. |
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Afiliação: |
PATRÍCIO FERREIRA BATISTA; MARIA AUXILIADORA COELHO DE LIMA, CPATSA; PATRICIA COELHO DE SOUZA LEAO, CPATSA; SORMANI ROBERTO ROSATTI; ANA CLAUDIA BARROS DOS SANTOS; RICARDO ELESBAO ALVES, SRI. |
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Título: |
Bioactive compounds and antioxidant activity in table grape cultivars from germplasm bank of Embrapa Tropical Semi-Arid. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON GRAPEVINE PHYSIOLOGY AND BIOTECHNOLOGY, 9., 2013, La Serena. Book of abstracts... La Serena, Chile: INIA: ISHS, 2013. |
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Páginas: |
p. 182. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to quantify the bioactive compounds content and the total antioxidant activity of 18 cultivars oftable grapes from the Active Germplasm Bank of Embrapa Tropical Semi-Aríd, located at the Experimental Field ofMandacaru, in Juazeiro, Bahia State, Brazil. The cultivars studied were: A dona, Benitaka, Brasil, Cardinal, Christmas Rose, Estevão Marinho, Frankenthal, Isabel, Isabel Precoce, Isaura, Liberty, Muscat Caillaba, Muscat Hamburg, Muscat Noir, Patrícia, Piratininga, Satum and Vênus. |
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Palavras-Chave: |
Atividade antioxidante; Compostos bioativos; Uva de mesa. |
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Thesagro: |
Uva; Vitis Vinifera. |
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Thesaurus Nal: |
Grapes. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136361/1/ID-50441.pdf
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Marc: |
LEADER 01356nam a2200253 a 4500
001 1961829
005 2023-05-08
008 2013 bl uuuu u00u1 u #d
100 1 $aBATISTA, P. F.
245 $aBioactive compounds and antioxidant activity in table grape cultivars from germplasm bank of Embrapa Tropical Semi-Arid.
260 $aIn: INTERNATIONAL SYMPOSIUM ON GRAPEVINE PHYSIOLOGY AND BIOTECHNOLOGY, 9., 2013, La Serena. Book of abstracts... La Serena, Chile: INIA: ISHS$c2013
300 $ap. 182.
520 $aThe aim of this study was to quantify the bioactive compounds content and the total antioxidant activity of 18 cultivars oftable grapes from the Active Germplasm Bank of Embrapa Tropical Semi-Aríd, located at the Experimental Field ofMandacaru, in Juazeiro, Bahia State, Brazil. The cultivars studied were: A dona, Benitaka, Brasil, Cardinal, Christmas Rose, Estevão Marinho, Frankenthal, Isabel, Isabel Precoce, Isaura, Liberty, Muscat Caillaba, Muscat Hamburg, Muscat Noir, Patrícia, Piratininga, Satum and Vênus.
650 $aGrapes
650 $aUva
650 $aVitis Vinifera
653 $aAtividade antioxidante
653 $aCompostos bioativos
653 $aUva de mesa
700 1 $aLIMA, M. A. C. de
700 1 $aLEAO, P. C. de S.
700 1 $aROSATTI, S. R.
700 1 $aSANTOS, A. C. B. dos
700 1 $aALVES, R. E.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
21/12/2022 |
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Data da última atualização: |
21/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
RECCHIA, K.; MACHADO, L. S.; BOTIGELLI, R. C.; PIERI, N. C. G.; BARBOSA, G.; CASTRO, R. V. G. de; MARQUES, M. G.; PESSÔA, L. V. de F.; FANTINATO NETO, P.; MEIRELLES, F. V.; SOUZA, A. F. de; MARTINS, S. M. M. K.; BRESSAN, F. F. |
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Afiliação: |
KAIANA RECCHIA, Universidade de São Paulo; LUCAS SIMÕES MACHADO, Universidade de São Paulo; RAMON CESAR BOTIGELLI, Universidade Estadual Paulista; NAIRA CAROLINE GODOY PIERI, Universidade de São Paulo; GABRIELA BARBOSA, Universidade de São Paulo; RAQUEL VASCONCELOS GUIMARÃES DE CASTRO, Universidade de São Paulo; MARIANA GROKE MARQUES, CNPSA; LAÍS VICARI DE FIGUEIREDO PESSÔA, Universidade de São Paulo; PAULO FANTINATO NETO, Universidade de São Paulo; FLÁVIO VIEIRA MEIRELLES, Universidade de São Paulo; ALINE FERNANDA DE SOUZA, Universidade de São Paulo; SIMONE MARIA MASSAMI KITAMURA MARTINS, Universidade de São Paulo; FABIANA FERNANDES BRESSAN, Universidade de São Paulo. |
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Título: |
In vitro induced pluripotency from urine-derived cells in porcine. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
World Journal of Stem Cells, v. 14, n. 3, p. 231-244, 2022. |
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DOI: |
https://doi.org/10.4252/wjsc.v14.i3.231 |
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Idioma: |
Inglês |
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Conteúdo: |
Methods: The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4 (OCT4), SRY-box2 (SOX2), Kruppel-like factor 4 (KLF4), and C-MYC, and cultured with basic fibroblast growth factor (bFGF) supplementation. To characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase (AP), OCT4, SOX2, NANOG, TRA1 81 and SSEA 1 detection. Endogenous transcripts related to the pluripotency (OCT4, SOX2 and NANOG) were analyzed via reverse transcription quantitative real-time polymerase chain reaction in different time points during the culture, and all three lineages formed embryoid bodies (EBs) when cultured in suspension without bFGF supplementation. Results: The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro reprogramming. Colonies of putative iPSCs were obtained only from UDCs transduced with the murine factors (mOSKM), but not from human factors (hOSKM). Three clonal lineages were isolated and further cultured for at least 28 passages, all the lineages were positive for AP detection, the OCT4, SOX2, NANOG markers, albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1, similar results were observed in the abundance of the endogenous transcripts related to pluripotent state. All the clonal lineages when cultured in suspension without bFGF were able to form EBs expressing ectoderm and mesoderm layers transcripts. Conclusion: For the first time UDCs were isolated in the swine model and reprogrammed into a pluripotent-like state, enabling new numerous applications in both human or veterinary regenerative medicine. MenosMethods: The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4 (OCT4), SRY-box2 (SOX2), Kruppel-like factor 4 (KLF4), and C-MYC, and cultured with basic fibroblast growth factor (bFGF) supplementation. To characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase (AP), OCT4, SOX2, NANOG, TRA1 81 and SSEA 1 detection. Endogenous transcripts related to the pluripotency (OCT4, SOX2 and NANOG) were analyzed via reverse transcription quantitative real-time polymerase chain reaction in different time points during the culture, and all three lineages formed embryoid bodies (EBs) when cultured in suspension without bFGF supplementation. Results: The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro reprogramming. Colonies of putative iPSCs were obtained only from UDCs transduced with the murine factors (mOSKM), but not from human factors (hOSKM). Three clonal lineages were isolated and further cultured for at least 28 passages, all the lineages were positive for AP detection, the OCT4, SOX2, NANOG markers, albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1, similar results were observed in the abundance of the endogenous transcripts related to pluripotent state. All the clonal lineages when cultured in suspension without bFGF were able to form EBs expressi... Mostrar Tudo |
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Palavras-Chave: |
Células-tronco; IPSC; Noninvasive; Pluripotência; Pluripotency; Reprogramming. |
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Thesagro: |
Suíno; Urina. |
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Thesaurus NAL: |
Induced pluripotent stem cells; Swine; Urine. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150134/1/10084.pdf
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Marc: |
LEADER 02880naa a2200409 a 4500
001 2150134
005 2022-12-21
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4252/wjsc.v14.i3.231$2DOI
100 1 $aRECCHIA, K.
245 $aIn vitro induced pluripotency from urine-derived cells in porcine.$h[electronic resource]
260 $c2022
520 $aMethods: The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4 (OCT4), SRY-box2 (SOX2), Kruppel-like factor 4 (KLF4), and C-MYC, and cultured with basic fibroblast growth factor (bFGF) supplementation. To characterize the putative porcine iPSCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase (AP), OCT4, SOX2, NANOG, TRA1 81 and SSEA 1 detection. Endogenous transcripts related to the pluripotency (OCT4, SOX2 and NANOG) were analyzed via reverse transcription quantitative real-time polymerase chain reaction in different time points during the culture, and all three lineages formed embryoid bodies (EBs) when cultured in suspension without bFGF supplementation. Results: The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro reprogramming. Colonies of putative iPSCs were obtained only from UDCs transduced with the murine factors (mOSKM), but not from human factors (hOSKM). Three clonal lineages were isolated and further cultured for at least 28 passages, all the lineages were positive for AP detection, the OCT4, SOX2, NANOG markers, albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and SSEA1, similar results were observed in the abundance of the endogenous transcripts related to pluripotent state. All the clonal lineages when cultured in suspension without bFGF were able to form EBs expressing ectoderm and mesoderm layers transcripts. Conclusion: For the first time UDCs were isolated in the swine model and reprogrammed into a pluripotent-like state, enabling new numerous applications in both human or veterinary regenerative medicine.
650 $aInduced pluripotent stem cells
650 $aSwine
650 $aUrine
650 $aSuíno
650 $aUrina
653 $aCélulas-tronco
653 $aIPSC
653 $aNoninvasive
653 $aPluripotência
653 $aPluripotency
653 $aReprogramming
700 1 $aMACHADO, L. S.
700 1 $aBOTIGELLI, R. C.
700 1 $aPIERI, N. C. G.
700 1 $aBARBOSA, G.
700 1 $aCASTRO, R. V. G. de
700 1 $aMARQUES, M. G.
700 1 $aPESSÔA, L. V. de F.
700 1 $aFANTINATO NETO, P.
700 1 $aMEIRELLES, F. V.
700 1 $aSOUZA, A. F. de
700 1 $aMARTINS, S. M. M. K.
700 1 $aBRESSAN, F. F.
773 $tWorld Journal of Stem Cells$gv. 14, n. 3, p. 231-244, 2022.
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Embrapa Suínos e Aves (CNPSA) |
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