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| Registros recuperados : 156 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
10/12/2019 |
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Data da última atualização: |
02/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CARVALHO, B. P.; COSTA, F. de Q.; DETONI, D.; ROSA, F. B.; DIAS, A. J. B. |
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Afiliação: |
BRUNO PENA CARVALHO, CPAF-AC; Fernanda de Queirós Costa, Universidade Estadual do Norte Fluminense Darcy Ribeiro; Danielly Detoni, Universidade Estadual do Norte Fluminense Darcy Ribeiro; Felipe Borges Rosa, Universidade Estadual do Norte Fluminense Darcy Ribeiro; Angelo José Burla Dias, Universidade Estadual do Norte Fluminense Darcy Ribeiro. |
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Título: |
Use of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos: effect on cryoresistance and lipid content. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 48, e20180322, set. 2019. |
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ISSN: |
1806-9290 |
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DOI: |
10.1590/rbz4820180322 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro in medium supplemented with CLA (100 mM) in the first 72 h (CLA-F), last 72 h (CLA-L), or throughout the culture period (CLA-T). Control embryos (control) were cultivated with no CLA. Embryos were cryopreserved by vitrification for subsequent analysis after devitrification. Effect of CLA on cryoresistance was assessed by cultivating embryos in synthetic oviductal fluid containing 5% fetal bovine serum. Lipid content was quantified using Nile Red staining. No significant difference was observed in cleavage rate, blastocyst:total oocyte ratio, and blastocyst:cleaved oocyte ratio. Culture in CLA-L reduced survival rate 24 h after devitrification compared with CLA-F and CLA-T, although with no statistically significant difference compared with control group. However, CLA-T improved embryo hatching rate and affected lipid content of embryos. Cultures CLA-F and CLA-L increased lipid content compared with control. Yet, lipid content values decreased in embryos treated with CLA-T, but they did not differ significantly from the values observed for oocytes at the germinal vesicle stage. Treatment of bovine embryos with CLA during in vitro cultivation did not affect the production of blastocysts, reducing lipid content and improving cryoresistance. However, the effects of CLA on cryoresistance and lipid content is significant only when embryos are exposed to the compound throughout the cultivation period. MenosThis study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro in medium supplemented with CLA (100 mM) in the first 72 h (CLA-F), last 72 h (CLA-L), or throughout the culture period (CLA-T). Control embryos (control) were cultivated with no CLA. Embryos were cryopreserved by vitrification for subsequent analysis after devitrification. Effect of CLA on cryoresistance was assessed by cultivating embryos in synthetic oviductal fluid containing 5% fetal bovine serum. Lipid content was quantified using Nile Red staining. No significant difference was observed in cleavage rate, blastocyst:total oocyte ratio, and blastocyst:cleaved oocyte ratio. Culture in CLA-L reduced survival rate 24 h after devitrification compared with CLA-F and CLA-T, although with no statistically significant difference compared with control group. However, CLA-T improved embryo hatching rate and affected lipid content of embryos. Cultures CLA-F and CLA-L increased lipid content compared with control. Yet, lipid content values decreased in embryos treated with CLA-T, but they did not differ significantly from the values observed for oocytes at the germinal vesicle stage. Treatment of bovine embryos with CLA during in vitro ... Mostrar Tudo |
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Palavras-Chave: |
Ácido graxo poliinsaturado; Ácido linoleíco conjugado; Ácidos grasos polisaturados; Contenido de lípidos; Criopreservación; Cultivo de embriones; Embrión (animal); Ganado bovino; Oocyte vitrification; Reproducción de animales; Tolerancia al frío; Vitrificación de ovocitos. |
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Thesagro: |
Bovino; Congelamento; Criopreservação; Cultura In Vitro; Embrião Animal; Lipídio; Reprodução Animal; Resistência a Temperatura. |
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Thesaurus NAL: |
Animal reproduction; Cattle; Cold tolerance; Conjugated linoleic acid; Cryopreservation; Embryo (animal); Embryo culture; Lipid content; Polyunsaturated fatty acids. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/206636/1/26920.pdf
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Marc: |
LEADER 03409naa a2200541 a 4500
001 2116519
005 2021-07-02
008 2019 bl uuuu u00u1 u #d
022 $a1806-9290
024 7 $a10.1590/rbz4820180322$2DOI
100 1 $aCARVALHO, B. P.
245 $aUse of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryos$beffect on cryoresistance and lipid content.$h[electronic resource]
260 $c2019
520 $aThis study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro in medium supplemented with CLA (100 mM) in the first 72 h (CLA-F), last 72 h (CLA-L), or throughout the culture period (CLA-T). Control embryos (control) were cultivated with no CLA. Embryos were cryopreserved by vitrification for subsequent analysis after devitrification. Effect of CLA on cryoresistance was assessed by cultivating embryos in synthetic oviductal fluid containing 5% fetal bovine serum. Lipid content was quantified using Nile Red staining. No significant difference was observed in cleavage rate, blastocyst:total oocyte ratio, and blastocyst:cleaved oocyte ratio. Culture in CLA-L reduced survival rate 24 h after devitrification compared with CLA-F and CLA-T, although with no statistically significant difference compared with control group. However, CLA-T improved embryo hatching rate and affected lipid content of embryos. Cultures CLA-F and CLA-L increased lipid content compared with control. Yet, lipid content values decreased in embryos treated with CLA-T, but they did not differ significantly from the values observed for oocytes at the germinal vesicle stage. Treatment of bovine embryos with CLA during in vitro cultivation did not affect the production of blastocysts, reducing lipid content and improving cryoresistance. However, the effects of CLA on cryoresistance and lipid content is significant only when embryos are exposed to the compound throughout the cultivation period.
650 $aAnimal reproduction
650 $aCattle
650 $aCold tolerance
650 $aConjugated linoleic acid
650 $aCryopreservation
650 $aEmbryo (animal)
650 $aEmbryo culture
650 $aLipid content
650 $aPolyunsaturated fatty acids
650 $aBovino
650 $aCongelamento
650 $aCriopreservação
650 $aCultura In Vitro
650 $aEmbrião Animal
650 $aLipídio
650 $aReprodução Animal
650 $aResistência a Temperatura
653 $aÁcido graxo poliinsaturado
653 $aÁcido linoleíco conjugado
653 $aÁcidos grasos polisaturados
653 $aContenido de lípidos
653 $aCriopreservación
653 $aCultivo de embriones
653 $aEmbrión (animal)
653 $aGanado bovino
653 $aOocyte vitrification
653 $aReproducción de animales
653 $aTolerancia al frío
653 $aVitrificación de ovocitos
700 1 $aCOSTA, F. de Q.
700 1 $aDETONI, D.
700 1 $aROSA, F. B.
700 1 $aDIAS, A. J. B.
773 $tRevista Brasileira de Zootecnia$gv. 48, e20180322, set. 2019.
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Embrapa Acre (CPAF-AC) |
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