03409naa a2200541 a 450000100080000000500110000800800410001902200140006002400310007410000200010524501510012526000090027652017740028565000240205965000110208365000190209465000290211365000210214265000200216365000190218365000180220265000320222065000110225265000170226365000220228065000210230265000200232365000130234365000240235665000310238065300320241165300320244365300330247565300260250865300220253465300250255665300220258165300180260365300250262165300300264665300240267665300310270070000200273170000150275170000160276670000190278277300660280121165192021-07-02       2019    bl uuuu     u00u1 u    #d  a1806-92907 a10.1590/rbz48201803222DOI1 aCARVALHO, B. P.  aUse of conjugated linoleic acid (trans 10, cis 12) to cultivate bovine embryosbeffect on cryoresistance and lipid content.h[electronic resource]  c2019  aThis study evaluated the effect of the addition of conjugated linoleic acid (CLA) to in vitro culture on viability, lipid content, and cryoresistance of bovine embryos at different in vitro culture times. Cumulus oocyte complexes (N = 974) were maturated in vitro for 22 h. In vitro fecundation ensued for 18 h. Viable zygotes were cultivated in vitro in medium supplemented with CLA (100 mM) in the first 72 h (CLA-F), last 72 h (CLA-L), or throughout the culture period (CLA-T). Control embryos (control) were cultivated with no CLA. Embryos were cryopreserved by vitrification for subsequent analysis after devitrification. Effect of CLA on cryoresistance was assessed by cultivating embryos in synthetic oviductal fluid containing 5% fetal bovine serum. Lipid content was quantified using Nile Red staining. No significant difference was observed in cleavage rate, blastocyst:total oocyte ratio, and blastocyst:cleaved oocyte ratio. Culture in CLA-L reduced survival rate 24 h after devitrification compared with CLA-F and CLA-T, although with no statistically significant difference compared with control group. However, CLA-T improved embryo hatching rate and affected lipid content of embryos. Cultures CLA-F and CLA-L increased lipid content compared with control. Yet, lipid content values decreased in embryos treated with CLA-T, but they did not differ significantly from the values observed for oocytes at the germinal vesicle stage. Treatment of bovine embryos with CLA during in vitro cultivation did not affect the production of blastocysts, reducing lipid content and improving cryoresistance. However, the effects of CLA on cryoresistance and lipid content is significant only when embryos are exposed to the compound throughout the cultivation period.  aAnimal reproduction  aCattle  aCold tolerance  aConjugated linoleic acid  aCryopreservation  aEmbryo (animal)  aEmbryo culture  aLipid content  aPolyunsaturated fatty acids  aBovino  aCongelamento  aCriopreservação  aCultura In Vitro  aEmbrião Animal  aLipídio  aReprodução Animal  aResistência a Temperatura  aÁcido graxo poliinsaturado  aÁcido linoleíco conjugado  aÁcidos grasos polisaturados  aContenido de lípidos  aCriopreservación  aCultivo de embriones  aEmbrión (animal)  aGanado bovino  aOocyte vitrification  aReproducción de animales  aTolerancia al frío  aVitrificación de ovocitos1 aCOSTA, F. de Q.1 aDETONI, D.1 aROSA, F. B.1 aDIAS, A. J. B.  tRevista Brasileira de Zootecniagv. 48, e20180322, set. 2019.