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26. | | SCHAEFER, R.; GAVA, D.; HAACH, V.; ZANELLA, J. R. C.; CANTAO, M. E. Novel H1N2 swine influenza virus reassortant strain derived from the pandemic H1N1/2009 virus. In: CONGRESSO BRASILEIRO DE VIROLOGIA, 29.; ENCONTRO DE VIROLOGIA DO MERCOSUL, 13., 2018, Gramado. Resumos... Brasília: SBV, 2018. Biblioteca(s): Embrapa Suínos e Aves. |
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27. | | SCHAEFER, R.; GAVA, D.; HAACH, V.; CANTAO, M. E.; NELSON, M. I. Novel human-like H1N1 swine influenza virus with the six internal genes derived from H1N1/2009 virus. In: CONGRESSO BRASILEIRO DE VIROLOGIA, 28.; ENCONTRO DE VIROLOGIA DO MERCOSUL, 12., 2017, Belo Horizonte. Trabalho pôsteres... Brasília: SBV, 2017. Biblioteca(s): Embrapa Suínos e Aves. |
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29. | | DOMENECH, D.; CANTAO, M. E.; COSTA, O. Y.; CARVALHO B. J.; KYAW, C. M.; KRUGER, R. H.; QUIRINO, B. F. Comparative characterization of the archaeal community in the amazon forest: native forest and oil palm plantation by high-throughput sequencing. In: CONGRESSO LATINOAMERICANO DE MICROBIOLOGIA, 22.; CONGRESSO BOLIVIANO DE MICROBIOLOGIA, 4., 2014, Cartagena. Memórias: Clínica- Bioanálisis - Industrial - Ambiental. Cartagena: Universidad de Antioquia; Associação Colombiana de Microbiologia, 2014. p. 87. Publicado em: Hechos Microbiológicos, v. 5. n. 2, suplemento 2, p. 62, noviembre, 2014. p. 87. ALAM 2014; CCM 2014. Biblioteca(s): Embrapa Agroenergia; Embrapa Suínos e Aves. |
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30. | | ZANELLA, R.; CANTAO, M. E.; PEIXOTO, J. de O.; PANDOLFI, J. R. C.; COUTINHO, L. L.; LEDUR, M. C. Collaborative effort to identify genes involved with bone related traits in broiler chickens. In: INTERNATIONAL PLANT & ANIMAL GENOME, 21., 2013, San Diego. Abstracts... Jersey City: Scherago International, 2013. P0640. Biblioteca(s): Embrapa Suínos e Aves. |
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31. | | GAVA, D.; SERRÃO, V. H. B.; FERNANDES, L. T.; CANTAO, M. E.; ZANELLA, J. R. C.; MORES, N.; SCHAEFER, R. Structure analysis of capsid protein of porcine circovirus type 2 from pigs with systemic disease. Brazilian Journal of Microbiology, v. 49, p. 351-357, 2018. Biblioteca(s): Embrapa Suínos e Aves. |
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32. | | GAVA, D.; SOUZA, C. K.; SCHAEFER, R.; VINCENT, A. L.; CANTAO, M. E.; COLDEBELLA, A.; ZANELLA, J. R. C. A TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4. Journal of Virological Methods, v. 219, p. 14-17, 2015. Biblioteca(s): Embrapa Suínos e Aves. |
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34. | | TUPINAMBA, D.; CANTAO, M. E.; COSTA, O. Y.; BERGMANN, J. C.; KYAW, C.; BARRETO, C.; QUIRINO, B. F. High-throughput sequencing characterization of land use impact on archaeal community: Amazon native forest and oil palm plantation. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 37., 2015, San Diego, California. [Resumos ...] Fairfax: Society for Industrial Microbiology and Biotechnology, 2015. Resumo T67 Biblioteca(s): Embrapa Agroenergia. |
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35. | | SCHAEFER, R.; GAVA, D.; HAACH, V.; SERRÃO, V. H. B.; CANTAO, M. E.; ZANELLA, J. R. C. Genetic diversity of porcine circovirus type 2 causing clinical disease in Brazilian pig herds concomitant with PCV2 vaccination. Virus Reviews & Research, Brasília, DF, v. 20, p. 247-248, Oct. 2015. Supplement 1, ref. VV335. Edição dos Resumos do XXVI Brazilian Congress of Virology, X Mercosur Meeting of Virology, Florianóplis, SC, Oct. 2015. Biblioteca(s): Embrapa Suínos e Aves. |
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Registro Completo
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
04/12/2017 |
Data da última atualização: |
04/12/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
VIANCELLI, A.; PRA, M. C. de; SCUSSIATO, L. A.; CANTAO, M. E.; IBELLI, A. M. G.; KUNZ, A. |
Afiliação: |
ALINE VIANCELLI, UNC/Concórdia; MARINA CELANT DE PRÁ; LUCAS ANTUNES SCUSSIATO, UNIOESTE/Cascavel; MAURICIO EGIDIO CANTAO, CNPSA; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; AIRTON KUNZ, CNPSA. |
Título: |
Preservation and reactivation of candidatus jettenia asiatica and anammoxoglobus propionicus using different preservative agents. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Chemosphere, v. 186, p. 453-458, 2017. |
DOI: |
10.1016/j.chemosphere.2017.07.053 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Anaerobic ammonium oxidation (anammox) bacteria have peculiar characteristics that make them difficult to cultivate. The conservation of these microorganisms in culture collections or laboratories requires successful preservation and reactivation techniques. Furthermore, studies have shown that successful reactivation may be preservative dependent. Considering this, the present study aimed to evaluate the preservation and reactivation of anammox consortia enriched from swine manure treatment lagoons, by using different preservative agents at different temperatures: KNO3 (at 4 C), glycerol (20 C, 80 C), and skimmed cow milk (20 C, 80 C, 200 C). After 4 months, the biomass was thawed (except for KNO3), and the reestablishment of anammox activity was evaluated by stoichiometric coefficients. Microbial community transformation during the reactivation process was also studied by 16S rDNA sequence analysis. The results showed that the anammox biomass preserved with glycerol or skimmed cow milk at 80 C recovered activity, while the biomass preserved with other methodologies did not reestablish activity during the studied time (90 days). The bacterial community from the biomass with anammox activity was characterized and showed the presence of Candidatus Brocadia anammoxidans, Candidatus Jettenia asiatica, and Candidatus Anammoxoglobus propionicus. Preservation with skimmed cow milk at 80 C favored the selection of Candidatus Anammoxoglobus propionicus, while preservation with glycerol at 80 C was successful for Candidatus Jettenia asiatica. The present study was effective on anammox sludge preservation and reactivation using low-cost processes for anammox cultures preservation, which is important for biomass transport and deammonification reactor start up. © 2017 MenosAbstract: Anaerobic ammonium oxidation (anammox) bacteria have peculiar characteristics that make them difficult to cultivate. The conservation of these microorganisms in culture collections or laboratories requires successful preservation and reactivation techniques. Furthermore, studies have shown that successful reactivation may be preservative dependent. Considering this, the present study aimed to evaluate the preservation and reactivation of anammox consortia enriched from swine manure treatment lagoons, by using different preservative agents at different temperatures: KNO3 (at 4 C), glycerol (20 C, 80 C), and skimmed cow milk (20 C, 80 C, 200 C). After 4 months, the biomass was thawed (except for KNO3), and the reestablishment of anammox activity was evaluated by stoichiometric coefficients. Microbial community transformation during the reactivation process was also studied by 16S rDNA sequence analysis. The results showed that the anammox biomass preserved with glycerol or skimmed cow milk at 80 C recovered activity, while the biomass preserved with other methodologies did not reestablish activity during the studied time (90 days). The bacterial community from the biomass with anammox activity was characterized and showed the presence of Candidatus Brocadia anammoxidans, Candidatus Jettenia asiatica, and Candidatus Anammoxoglobus propionicus. Preservation with skimmed cow milk at 80 C favored the selection of Candidatus Anammoxoglobus propionicus, while preservation ... Mostrar Tudo |
Palavras-Chave: |
Anammox; Gestão ambiental; Lodo. |
Thesagro: |
Meio ambiente. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02526naa a2200241 a 4500 001 2081303 005 2017-12-04 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1016/j.chemosphere.2017.07.053$2DOI 100 1 $aVIANCELLI, A. 245 $aPreservation and reactivation of candidatus jettenia asiatica and anammoxoglobus propionicus using different preservative agents.$h[electronic resource] 260 $c2017 520 $aAbstract: Anaerobic ammonium oxidation (anammox) bacteria have peculiar characteristics that make them difficult to cultivate. The conservation of these microorganisms in culture collections or laboratories requires successful preservation and reactivation techniques. Furthermore, studies have shown that successful reactivation may be preservative dependent. Considering this, the present study aimed to evaluate the preservation and reactivation of anammox consortia enriched from swine manure treatment lagoons, by using different preservative agents at different temperatures: KNO3 (at 4 C), glycerol (20 C, 80 C), and skimmed cow milk (20 C, 80 C, 200 C). After 4 months, the biomass was thawed (except for KNO3), and the reestablishment of anammox activity was evaluated by stoichiometric coefficients. Microbial community transformation during the reactivation process was also studied by 16S rDNA sequence analysis. The results showed that the anammox biomass preserved with glycerol or skimmed cow milk at 80 C recovered activity, while the biomass preserved with other methodologies did not reestablish activity during the studied time (90 days). The bacterial community from the biomass with anammox activity was characterized and showed the presence of Candidatus Brocadia anammoxidans, Candidatus Jettenia asiatica, and Candidatus Anammoxoglobus propionicus. Preservation with skimmed cow milk at 80 C favored the selection of Candidatus Anammoxoglobus propionicus, while preservation with glycerol at 80 C was successful for Candidatus Jettenia asiatica. The present study was effective on anammox sludge preservation and reactivation using low-cost processes for anammox cultures preservation, which is important for biomass transport and deammonification reactor start up. © 2017 650 $aMeio ambiente 653 $aAnammox 653 $aGestão ambiental 653 $aLodo 700 1 $aPRA, M. C. de 700 1 $aSCUSSIATO, L. A. 700 1 $aCANTAO, M. E. 700 1 $aIBELLI, A. M. G. 700 1 $aKUNZ, A. 773 $tChemosphere$gv. 186, p. 453-458, 2017.
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