02526naa a2200241 a 450000100080000000500110000800800410001902400430006010000180010324501570012126000090027852018020028765000180208965300120210765300220211965300090214170000180215070000210216870000180218970000210220770000130222877300430224120813032017-12-04       2017    bl uuuu     u00u1 u    #d7 a10.1016/j.chemosphere.2017.07.0532DOI1 aVIANCELLI, A.  aPreservation and reactivation of candidatus jettenia asiatica and anammoxoglobus propionicus using different preservative agents.h[electronic resource]  c2017  aAbstract: Anaerobic ammonium oxidation (anammox) bacteria have peculiar characteristics that make them difficult to cultivate. The conservation of these microorganisms in culture collections or laboratories requires successful preservation and reactivation techniques. Furthermore, studies have shown that successful reactivation may be preservative dependent. Considering this, the present study aimed to evaluate the preservation and reactivation of anammox consortia enriched from swine manure treatment lagoons, by using different preservative agents at different temperatures: KNO3 (at 4 C), glycerol (20 C, 80 C), and skimmed cow milk (20 C, 80 C, 200 C). After 4 months, the biomass was thawed (except for KNO3), and the reestablishment of anammox activity was evaluated by stoichiometric coefficients. Microbial community transformation during the reactivation process was also studied by 16S rDNA sequence analysis. The results showed that the anammox biomass preserved with glycerol or skimmed cow milk at 80 C recovered activity, while the biomass preserved with other methodologies did not reestablish activity during the studied time (90 days). The bacterial community from the biomass with anammox activity was characterized and showed the presence of Candidatus Brocadia anammoxidans, Candidatus Jettenia asiatica, and Candidatus Anammoxoglobus propionicus. Preservation with skimmed cow milk at 80 C favored the selection of Candidatus Anammoxoglobus propionicus, while preservation with glycerol at 80 C was successful for Candidatus Jettenia asiatica. The present study was effective on anammox sludge preservation and reactivation using low-cost processes for anammox cultures preservation, which is important for biomass transport and deammonification reactor start up. © 2017  aMeio ambiente  aAnammox  aGestão ambiental  aLodo1 aPRA, M. C. de1 aSCUSSIATO, L. A.1 aCANTAO, M. E.1 aIBELLI, A. M. G.1 aKUNZ, A.  tChemospheregv. 186, p. 453-458, 2017.