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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
WOHLRES-VIANA, S.; PEREIRA, M. M.; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. |
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Afiliação: |
SABINE WOHLRES-VIANA, UFJF; MICHELE MUNK PEREIRA, UFJF; JOAO HENRIQUE MOREIRA VIANA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Comparison of gene expression in Bos indicus and Bos taurus embryos produced in vivo or in vitro. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Livestock Science, v. 140, n. 1/3, p. 62-67, 2011. |
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DOI: |
https://doi.org/10.1016/j.livsci.2011.02.008 |
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Idioma: |
Inglês |
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Conteúdo: |
Bos taurus and Bos indicus are subspecies that differ in several aspects of their reproductive physiology, including the potential for in vivo and in vitro embryo production. Such differences may be originated from cellular and molecular adaptations to the environment. Despite the information available about gene expression of B. taurus embryos, little is known for B. indicus ones. This study aimed to compare the relative gene expression of B. taurus (Holstein) and B. indicus (Gyr) embryos produced in vivo or in vitro. For each subspecies, blastocysts produced in vivo and in vitro were used for expression analysis by Real-Time PCR of genes important for blastocyst formation. The following comparisons were performed: I) In vivo: Gyr vs. Holstein, II) In vitro: Gyr vs. Holstein, III) Gyr: in vivo vs. in vitro, and VI) Holstein: in vivo vs. in vitro. Aquaporin 3, Na/K-ATPase α1, and peroxiredoxin 1 genes were up-regulated (P < 0.05) for in vivo-produced Gyr embryos when compared to Holstein embryos, while there was no difference between breeds (P > 0.05) for in vitro-produced counterpart embryos. The Na/K-ATPase β2 and peroxiredoxin 1 genes were down-regulated (P < 0.05) for in vitro-produced Gyr embryos when compared to in vivo. No differences (P > 0.05) were found between in vitro and in vivo produced Holstein embryos. In conclusion, in vivo produced B. taurus and B. indicus blastocysts can have different expression pattern for some genes, but such differences can be attenuated in vitro, suggesting similar adaptive plasticity of the embryos to culture conditions, regardless of the subspecies. MenosBos taurus and Bos indicus are subspecies that differ in several aspects of their reproductive physiology, including the potential for in vivo and in vitro embryo production. Such differences may be originated from cellular and molecular adaptations to the environment. Despite the information available about gene expression of B. taurus embryos, little is known for B. indicus ones. This study aimed to compare the relative gene expression of B. taurus (Holstein) and B. indicus (Gyr) embryos produced in vivo or in vitro. For each subspecies, blastocysts produced in vivo and in vitro were used for expression analysis by Real-Time PCR of genes important for blastocyst formation. The following comparisons were performed: I) In vivo: Gyr vs. Holstein, II) In vitro: Gyr vs. Holstein, III) Gyr: in vivo vs. in vitro, and VI) Holstein: in vivo vs. in vitro. Aquaporin 3, Na/K-ATPase α1, and peroxiredoxin 1 genes were up-regulated (P < 0.05) for in vivo-produced Gyr embryos when compared to Holstein embryos, while there was no difference between breeds (P > 0.05) for in vitro-produced counterpart embryos. The Na/K-ATPase β2 and peroxiredoxin 1 genes were down-regulated (P < 0.05) for in vitro-produced Gyr embryos when compared to in vivo. No differences (P > 0.05) were found between in vitro and in vivo produced Holstein embryos. In conclusion, in vivo produced B. taurus and B. indicus blastocysts can have different expression pattern for some genes, but such differences can be attenuat... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Transcripts. |
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Thesaurus Nal: |
blastocyst. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02301naa a2200217 a 4500
001 1910633
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.livsci.2011.02.008$2DOI
100 1 $aWOHLRES-VIANA, S.
245 $aComparison of gene expression in Bos indicus and Bos taurus embryos produced in vivo or in vitro.$h[electronic resource]
260 $c2011
520 $aBos taurus and Bos indicus are subspecies that differ in several aspects of their reproductive physiology, including the potential for in vivo and in vitro embryo production. Such differences may be originated from cellular and molecular adaptations to the environment. Despite the information available about gene expression of B. taurus embryos, little is known for B. indicus ones. This study aimed to compare the relative gene expression of B. taurus (Holstein) and B. indicus (Gyr) embryos produced in vivo or in vitro. For each subspecies, blastocysts produced in vivo and in vitro were used for expression analysis by Real-Time PCR of genes important for blastocyst formation. The following comparisons were performed: I) In vivo: Gyr vs. Holstein, II) In vitro: Gyr vs. Holstein, III) Gyr: in vivo vs. in vitro, and VI) Holstein: in vivo vs. in vitro. Aquaporin 3, Na/K-ATPase α1, and peroxiredoxin 1 genes were up-regulated (P < 0.05) for in vivo-produced Gyr embryos when compared to Holstein embryos, while there was no difference between breeds (P > 0.05) for in vitro-produced counterpart embryos. The Na/K-ATPase β2 and peroxiredoxin 1 genes were down-regulated (P < 0.05) for in vitro-produced Gyr embryos when compared to in vivo. No differences (P > 0.05) were found between in vitro and in vivo produced Holstein embryos. In conclusion, in vivo produced B. taurus and B. indicus blastocysts can have different expression pattern for some genes, but such differences can be attenuated in vitro, suggesting similar adaptive plasticity of the embryos to culture conditions, regardless of the subspecies.
650 $ablastocyst
653 $aBovine
653 $aTranscripts
700 1 $aPEREIRA, M. M.
700 1 $aVIANA, J. H. M.
700 1 $aMACHADO, M. A.
700 1 $aCAMARGO, L. S. de A.
773 $tLivestock Science$gv. 140, n. 1/3, p. 62-67, 2011.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
24/11/2016 |
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Data da última atualização: |
24/11/2016 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
NASCIMENTO, T.; MARTINS, R. O.; BOMFIM, P. F.; VAZ, C. M. P. |
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Afiliação: |
PEDRO FERNANDES BOMFIM, CNPDIA; CARLOS MANOEL PEDRO VAZ, CNPDIA. |
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Título: |
Avaliação de um amostrador automático de solo com sensor de pressão para a medida da resistência do solo à penetração |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 8., 2016, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2016. p.31. Editores técnicos: Wilson Tadeu Lopes da Silva, José Manoel Marconcini, Maria Alice Martins, Lucimara Aparecida Forato, Paulino Ribeiro Villas Boas. (Embrapa Instrumentação. Documentos, 61). |
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Idioma: |
Português |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/150548/1/31.pdf
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Marc: |
LEADER 00787nam a2200133 a 4500
001 2057049
005 2016-11-24
008 2016 bl uuuu u00u1 u #d
100 1 $aNASCIMENTO, T.
245 $aAvaliação de um amostrador automático de solo com sensor de pressão para a medida da resistência do solo à penetração$h[electronic resource]
260 $aIn: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 8., 2016, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2016. p.31. Editores técnicos: Wilson Tadeu Lopes da Silva, José Manoel Marconcini, Maria Alice Martins, Lucimara Aparecida Forato, Paulino Ribeiro Villas Boas. (Embrapa Instrumentação. Documentos, 61).$c2016
700 1 $aMARTINS, R. O.
700 1 $aBOMFIM, P. F.
700 1 $aVAZ, C. M. P.
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