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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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Data corrente: |
07/01/2010 |
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Data da última atualização: |
25/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, F. A.; CARNEIRO, P. C. S.; CRUZ, C. D.; CARNEIRO, J. E. de S.; GUIMARAES, C. T. |
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Afiliação: |
FRANCIELLE ALLINE MARTINS, UFV; PEDRO CRESCÊNCIO SOUZA CARNEIRO, UFV; COSME DAMIÃO CRUZ, UFV; JOSÉ EUSTÁQUIO DE SOUZA CARNEIRO, UFV; CLAUDIA TEIXEIRA GUIMARAES, CNPMS. |
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Título: |
Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 10, p. 1291-1296, out. 2009. |
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DOI: |
10.1590/S0100-204X2009001000012 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations. |
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Palavras-Chave: |
Allelic origin; Heterozigoto; Heterozygote; Natural populations; Origem alélica; PCR semiquantitativa; Poligenes; Polygenes; Populações naturais; Semiquantitative PCR. |
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Thesagro: |
Zea Mays. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160668/1/Endosperm-genotyping.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE-2010/47247/1/44n10a12.pdf
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Marc: |
LEADER 01894naa a2200313 a 4500
001 1580267
005 2018-05-25
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1590/S0100-204X2009001000012$2DOI
100 1 $aMARTINS, F. A.
245 $aEndosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny.
260 $c2009
520 $aThe objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations.
650 $aZea Mays
653 $aAllelic origin
653 $aHeterozigoto
653 $aHeterozygote
653 $aNatural populations
653 $aOrigem alélica
653 $aPCR semiquantitativa
653 $aPoligenes
653 $aPolygenes
653 $aPopulações naturais
653 $aSemiquantitative PCR
700 1 $aCARNEIRO, P. C. S.
700 1 $aCRUZ, C. D.
700 1 $aCARNEIRO, J. E. de S.
700 1 $aGUIMARAES, C. T.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 44, n. 10, p. 1291-1296, out. 2009.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/08/2021 |
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Data da última atualização: |
19/08/2021 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
RIBEIRO, L. dos S.; BRANDÃO, F. Z.; CARVALHEIRA, L. de R.; LEMOS, D. R. de; SOUZA, G. T. de; BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; CARVALHO, B. C. de. |
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Afiliação: |
LILIAN DOS SANTOS RIBEIRO, Universidade Federal Fluminense; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense; LUCIANO DE REZENDE CARVALHEIRA, Universidade Federal de Minas Gerais; DIANA RANGEL DE LEMOS, Universidade Federal de Viçosa; GUSTAVO TORRES DE SOUZA, Universidade Federal de Juiz de Fora; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; BRUNO CAMPOS DE CARVALHO, CNPGL. |
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Título: |
Chromium supplementation modulates glucose metabolism in heatstressed Girolando dairy cows. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, v. 41, n. 5, p. 2445-2452, 2020. |
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DOI: |
https://doi.org/10.5433/1679-0359.2020v41n5Supl1p2445 |
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Idioma: |
Inglês |
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Notas: |
Short communications. |
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Conteúdo: |
The objective of this study was to evaluate the effects of dietary chromium supplementation on hepatic gene expression of lactating Girolando (Holstein x Gyr) cows under heat stress conditions in climatic chamber. Thirty-six ¾ Holstein x Gyr lactating cows were used, based on a 2x3 factorial scheme, to evaluate the effects of two diets (0 vs 0.50 mg of organic chromium kg-1 dry matter) and three environmental conditions (ECs): heat stress conditions in climatic chamber with ad libitum feeding (HS), a thermoneutral environment with ad libitum feeding (TN), and a pair-fed group in a thermoneutral environment (PF). Under HS group, the expression levels of glucose transporter 2 (GLUT2), glucose-6-phosphatase (G6Pase), and growth hormone receptor (rGH) were downregulated (P < 0.05) in chromium-supplemented cows compared to those in cows fed the control diet. GLUT2 expression was upregulated (P = 0.02) in the HS group and insulin-like growth factor 1 (IGF1) was downregulated (P < 0.01) in the PF group in cows fed the control diet compared to the expression in the TN group. No differences were observed between the ECs in terms of relative abundances of GLUT2, phosphoenolpyruvate carboxykinase (PEPCK), G6Pase, rGH, and IGF1 transcripts among the chromium-supplemented cows (P > 0.05). Heat stress caused changes in the expression of genes related to glucose metabolism, and organic chromium could modulate glucose metabolism in animals under heat stress conditions to some extent. |
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Palavras-Chave: |
Biópsia hepática; Crossbred cows; Glucose metabolism; Liver biopsy. |
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Thesagro: |
Gado Leiteiro; Gado Mestiço; Glicose; Metabolismo. |
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Thesaurus NAL: |
Dairy cattle. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02530naa a2200337 a 4500
001 2133450
005 2021-08-19
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.5433/1679-0359.2020v41n5Supl1p2445$2DOI
100 1 $aRIBEIRO, L. dos S.
245 $aChromium supplementation modulates glucose metabolism in heatstressed Girolando dairy cows.$h[electronic resource]
260 $c2020
500 $aShort communications.
520 $aThe objective of this study was to evaluate the effects of dietary chromium supplementation on hepatic gene expression of lactating Girolando (Holstein x Gyr) cows under heat stress conditions in climatic chamber. Thirty-six ¾ Holstein x Gyr lactating cows were used, based on a 2x3 factorial scheme, to evaluate the effects of two diets (0 vs 0.50 mg of organic chromium kg-1 dry matter) and three environmental conditions (ECs): heat stress conditions in climatic chamber with ad libitum feeding (HS), a thermoneutral environment with ad libitum feeding (TN), and a pair-fed group in a thermoneutral environment (PF). Under HS group, the expression levels of glucose transporter 2 (GLUT2), glucose-6-phosphatase (G6Pase), and growth hormone receptor (rGH) were downregulated (P < 0.05) in chromium-supplemented cows compared to those in cows fed the control diet. GLUT2 expression was upregulated (P = 0.02) in the HS group and insulin-like growth factor 1 (IGF1) was downregulated (P < 0.01) in the PF group in cows fed the control diet compared to the expression in the TN group. No differences were observed between the ECs in terms of relative abundances of GLUT2, phosphoenolpyruvate carboxykinase (PEPCK), G6Pase, rGH, and IGF1 transcripts among the chromium-supplemented cows (P > 0.05). Heat stress caused changes in the expression of genes related to glucose metabolism, and organic chromium could modulate glucose metabolism in animals under heat stress conditions to some extent.
650 $aDairy cattle
650 $aGado Leiteiro
650 $aGado Mestiço
650 $aGlicose
650 $aMetabolismo
653 $aBiópsia hepática
653 $aCrossbred cows
653 $aGlucose metabolism
653 $aLiver biopsy
700 1 $aBRANDÃO, F. Z.
700 1 $aCARVALHEIRA, L. de R.
700 1 $aLEMOS, D. R. de
700 1 $aSOUZA, G. T. de
700 1 $aBATISTA, R. I. T. P.
700 1 $aCAMARGO, L. S. de A.
700 1 $aCARVALHO, B. C. de
773 $tSemina: Ciências Agrárias$gv. 41, n. 5, p. 2445-2452, 2020.
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Embrapa Gado de Leite (CNPGL) |
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