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102. | | BARRETO, M. R.; SOUSA, M. do S. M. de; ADAIME, R.; ZUCCHI, R. A. Fruit flies in the Mato Grosso state, Brazil: Increasing knowledge about diversity, host plants and parasitoids. Revista Brasileira de Ciências Agrárias, v. 17, n. 2, p. 1-6, 2022. e1500. Biblioteca(s): Embrapa Amapá. |
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108. | | LOGUERCIO, L. L.; BARRETO, M. R.; SANTOS, C. G. dos; VALICENTE, F. H.; PAIVA, E. Padrão temporal de secreção de proteínas e atividade inseticida contra Spodoptera frugiperda (J. E. Smith) (Lepdoptera: noctuidae), no sobrenadante da cepa de Bacillus thuringiensis (Berlinger) 'HD125'. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 20., 1999, Salvador. Resumos... Salvador: SBM, 1999. Biblioteca(s): Embrapa Milho e Sorgo. |
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109. | | KÜNAS, T. B. S.; QUEIROZ, D. L. de; BURCKHARD, D.; BARRETO, M. R. Primeiro registro de Psyllaephagus blastopsyllae (Hymenoptera: Encyrtidae), parasitoide de Blastopsylla occidentalis (Hemiptera: Aphalaridae), no Mato Grosso, Brasil . Boletín de la Sociedad Entomológica Aragonesa, v. 66, p. 259-260, 2020. Nota científica. Biblioteca(s): Embrapa Florestas. |
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111. | | TOKUYAMA, R. M.; BARRETO, M.; HAMADA, H.; SCALOPPI, E. A. G.; FERREIRA, M. R. Validacao de um sistema de previsao de septoriose em tomateiro. Summa Phytopathologica, Piracicaba, v.27, n.1, p.101, jan./mar. 2001. Resumo. Biblioteca(s): Embrapa Hortaliças. |
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114. | | LOGUERCIO, L. L.; BARRETO, M. L.; ROCHA, T. L.; SANTOS, C. G.; TEIXEIRA, F. F.; PAIVA, E. Combined analysis of supernatant-based feeding bioassay and PCR as a first-tier screening strategy for Vip-derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm. Journal of Applied Microbiology, Danvers, v. 93, n. 2, p. 269-277, 2002. Biblioteca(s): Embrapa Milho e Sorgo. |
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116. | | GUIDO, S. I.; OLIVEIRA, M. A. L.; LIMA, P. F.; PAES BARRETO, M. B.; SOUZA, D. M. B. Sincronizacao do estro de cabras com progestagenos e eCG. In: CONGRESSO PERNAMBUCANO DE MEDICINA VETERINÁRIA, 4.; SEMINÁRIO NORDESTINO DE CAPRINO-OVINOCULTURA, 5., 1999, Recife. Anais... Recife: Sociedade Pernambucana de Medicina Veterinária, 1999. p.318-319. V Seminario Nordestino de Caprino-ovinocultura. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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117. | | BARRETO, M. C.; SILVA, P. C. G. da; CARVALHO, A. C. A. de; ALMEIDA, C. O. de; WANDER, A. E. Impactos socioeconômicos da dispersão da mosca-da-carambola (Bactrocera carambolae) à fruticultura nacional. In: SILVA, R. A. da; LEMOS, W. de P.; ZUCCHI, R. A. (Ed.). Moscas-das-frutas na Amazônia brasileira: diversidade, hospedeiros e inimigos naturais. Macapá: Embrapa Amapá, 2011. cap. 11. p. 185-195. Biblioteca(s): Embrapa Amapá; Embrapa Arroz e Feijão; Embrapa Mandioca e Fruticultura; Embrapa Semiárido. |
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118. | | VALICENTE, F. H.; BARRETO, M. R.; VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F. de; PAIVA, E. Identificação através de PCR dos genes Cryl de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae). Anais da Sociedade Entomológica do Brasil, Jaboticabal, v. 29, n. 1, p. 147-153, 2000. Biblioteca(s): Embrapa Milho e Sorgo. |
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119. | | MAZZARDO, T.; BARRETO, M. R.; PEZZINI, L. A.; SEDANO, A. D. B.; BURCKHARDT, D.; QUEIROZ, D. L. de. Diversidade de psilídeos (Hemiptera: Psylloidea) em Sorriso, MT. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 25., 2014, Goiânia. Entomologia integrada à sociedade para o desenvolvimento sustentável: trabalhos. [S.l.]: Sociedade Entomológica do Brasil, 2014. Disponibilizado online. Resumo. Biblioteca(s): Embrapa Florestas. |
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Registros recuperados : 192 | |
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Registro Completo
Biblioteca(s): |
Embrapa Amapá. |
Data corrente: |
29/11/2022 |
Data da última atualização: |
29/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
CAVALCANTE, M. de A.; OLIVEIRA, J. dos S.; BARRETO, M. S. da S.; PINHEIRO, L. P.; CANTUÁRIA, P. de C.; BORGES, W. L.; SILVA, G. A. da; SOUZA, T. M. de. |
Afiliação: |
MARÍLIA DE A. CAVALCANTE, INSTITUTO FEDERAL DO AMAPÁ; JANYNA DOS S. OLIVEIRA, INSTITUTO FEDERAL DO AMAPÁ; MAYRA S. DA S. BARRETO, UNIVERSIDADE DO ESTADO DO AMAPÁ; LUCAS P. PINHEIRO, UNIVERSIDADE DO ESTADO DO AMAPÁ; PATRICK DE C. CANTUÁRIA, INSTITUTO DE PESQUISAS CIENTÍFICAS E TECNOLÓGICAS DO ESTADO DO AMAPÁ; WARDSSON LUSTRINO BORGES, CPAF-AP; GABRIEL A. DA SILVA, UNIVERSIDADE DO ESTADO DO AMAPÁ; TIAGO MARCOLINO DE SOUZA, UNIVERSIDADE DO ESTADO DO AMAPÁ. |
Título: |
An HPLC method to determine phenolic compounds of plant extracts: application to Byrsonima crassifolia and Senna alata leaves. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Pharmacognosy Research, v. 14, n. 4, p. 395-404, 2022. |
DOI: |
10.5530/pres.14.4.58 |
Idioma: |
Inglês |
Conteúdo: |
Background: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work. MenosBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the... Mostrar Tudo |
Palavras-Chave: |
Compostos bioativos; Método cromatográfico. |
Thesagro: |
Cromatografia; Extrato Vegetal. |
Thesaurus NAL: |
Bioactive compounds; Plant extracts. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148941/1/CPAF-AP-AnHPLCMethod.pdf
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Marc: |
LEADER 02625naa a2200289 a 4500 001 2148941 005 2022-11-29 008 2022 bl uuuu u00u1 u #d 024 7 $a10.5530/pres.14.4.58$2DOI 100 1 $aCAVALCANTE, M. de A. 245 $aAn HPLC method to determine phenolic compounds of plant extracts$bapplication to Byrsonima crassifolia and Senna alata leaves.$h[electronic resource] 260 $c2022 520 $aBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work. 650 $aBioactive compounds 650 $aPlant extracts 650 $aCromatografia 650 $aExtrato Vegetal 653 $aCompostos bioativos 653 $aMétodo cromatográfico 700 1 $aOLIVEIRA, J. dos S. 700 1 $aBARRETO, M. S. da S. 700 1 $aPINHEIRO, L. P. 700 1 $aCANTUÁRIA, P. de C. 700 1 $aBORGES, W. L. 700 1 $aSILVA, G. A. da 700 1 $aSOUZA, T. M. de 773 $tPharmacognosy Research$gv. 14, n. 4, p. 395-404, 2022.
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