02625naa a2200289 a 450000100080000000500110000800800410001902400300006010000250009024501540011526000090026952017130027865000240199165000190201565000180203465000200205265300240207265300280209670000240212470000250214870000200217370000250219370000180221870000200223670000200225677300590227621489412022-11-29       2022    bl uuuu     u00u1 u    #d7 a10.5530/pres.14.4.582DOI1 aCAVALCANTE, M. de A.  aAn HPLC method to determine phenolic compounds of plant extractsbapplication to Byrsonima crassifolia and Senna alata leaves.h[electronic resource]  c2022  aBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work.  aBioactive compounds  aPlant extracts  aCromatografia  aExtrato Vegetal  aCompostos bioativos  aMétodo cromatográfico1 aOLIVEIRA, J. dos S.1 aBARRETO, M. S. da S.1 aPINHEIRO, L. P.1 aCANTUÁRIA, P. de C.1 aBORGES, W. L.1 aSILVA, G. A. da1 aSOUZA, T. M. de  tPharmacognosy Researchgv. 14, n. 4, p. 395-404, 2022.