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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
03/11/2014 |
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Data da última atualização: |
03/11/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FAJARDO, T. V. M.; NICKEL, O. |
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Afiliação: |
THOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV. |
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Título: |
Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Ciência Rural, Santa Maria, v. 44, n. 10, p. 1711-1714, out. 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was suffi ciently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specifi city was confi rmed by reaction with homologous viral cDNA, individually cloned for each virus. |
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Palavras-Chave: |
ACLSV; ApMV; ASGV; ASPV; Macieira; Polissonda. |
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Thesagro: |
Doença de planta; Hibridação; Maçã. |
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Thesaurus Nal: |
Apple chlorotic leaf spot virus; Apple mosaic virus; Apple stem grooving virus; Apple stem pitting virus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/110894/1/Fajardo-CienciaRural-2014.pdf
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Marc: |
LEADER 01501naa a2200289 a 4500
001 1999019
005 2014-11-03
008 2014 bl uuuu u00u1 u #d
100 1 $aFAJARDO, T. V. M.
245 $aSimultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.$h[electronic resource]
260 $c2014
520 $aThe viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was suffi ciently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specifi city was confi rmed by reaction with homologous viral cDNA, individually cloned for each virus.
650 $aApple chlorotic leaf spot virus
650 $aApple mosaic virus
650 $aApple stem grooving virus
650 $aApple stem pitting virus
650 $aDoença de planta
650 $aHibridação
650 $aMaçã
653 $aACLSV
653 $aApMV
653 $aASGV
653 $aASPV
653 $aMacieira
653 $aPolissonda
700 1 $aNICKEL, O.
773 $tCiência Rural, Santa Maria$gv. 44, n. 10, p. 1711-1714, out. 2014.
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Embrapa Uva e Vinho (CNPUV) |
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