01501naa a2200289 a 450000100080000000500110000800800410001910000220006024501370008226000090021952006630022865000360089165000230092765000300095065000290098065000220100965000170103165000110104865300100105965300090106965300090107865300090108765300130109665300150110970000150112477300720113919990192014-11-03       2014    bl uuuu     u00u1 u    #d1 aFAJARDO, T. V. M.  aSimultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe.h[electronic resource]  c2014  aThe viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was suffi ciently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specifi city was confi rmed by reaction with homologous viral cDNA, individually cloned for each virus.  aApple chlorotic leaf spot virus  aApple mosaic virus  aApple stem grooving virus  aApple stem pitting virus  aDoença de planta  aHibridação  aMaçã  aACLSV  aApMV  aASGV  aASPV  aMacieira  aPolissonda1 aNICKEL, O.  tCiência Rural, Santa Mariagv. 44, n. 10, p. 1711-1714, out. 2014.