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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
Data corrente: |
07/01/2010 |
Data da última atualização: |
25/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MARTINS, F. A.; CARNEIRO, P. C. S.; CRUZ, C. D.; CARNEIRO, J. E. de S.; GUIMARAES, C. T. |
Afiliação: |
FRANCIELLE ALLINE MARTINS, UFV; PEDRO CRESCÊNCIO SOUZA CARNEIRO, UFV; COSME DAMIÃO CRUZ, UFV; JOSÉ EUSTÁQUIO DE SOUZA CARNEIRO, UFV; CLAUDIA TEIXEIRA GUIMARAES, CNPMS. |
Título: |
Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 10, p. 1291-1296, out. 2009. |
DOI: |
10.1590/S0100-204X2009001000012 |
Idioma: |
Inglês |
Conteúdo: |
The objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations. |
Palavras-Chave: |
Allelic origin; Heterozigoto; Heterozygote; Natural populations; Origem alélica; PCR semiquantitativa; Poligenes; Polygenes; Populações naturais; Semiquantitative PCR. |
Thesagro: |
Zea Mays. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160668/1/Endosperm-genotyping.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE-2010/47247/1/44n10a12.pdf
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Marc: |
LEADER 01894naa a2200313 a 4500 001 1580267 005 2018-05-25 008 2009 bl uuuu u00u1 u #d 024 7 $a10.1590/S0100-204X2009001000012$2DOI 100 1 $aMARTINS, F. A. 245 $aEndosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny. 260 $c2009 520 $aThe objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations. 650 $aZea Mays 653 $aAllelic origin 653 $aHeterozigoto 653 $aHeterozygote 653 $aNatural populations 653 $aOrigem alélica 653 $aPCR semiquantitativa 653 $aPoligenes 653 $aPolygenes 653 $aPopulações naturais 653 $aSemiquantitative PCR 700 1 $aCARNEIRO, P. C. S. 700 1 $aCRUZ, C. D. 700 1 $aCARNEIRO, J. E. de S. 700 1 $aGUIMARAES, C. T. 773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 44, n. 10, p. 1291-1296, out. 2009.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registros recuperados : 3 | |
1. | | MARTINS, F. A.; CARNEIRO, P. C. S.; CRUZ, C. D.; CARNEIRO, J. E. de S.; GUIMARAES, C. T. Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 10, p. 1291-1296, out. 2009.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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2. | | SILVA, J. N. M.; LOPES, J. do C. A.; OLIVEIRA, L. C. de; SILVA, S. M. A. da; CARVALHO, J. O. P. de; COSTA, D. H. M.; MELO, M. S.; TAVARES, M. J. M. Diretrizes para instalação e medição de parcelas permanentes em florestas naturais da Amazônia brasileira. Belém, PA: Embrapa Amazônia Oriental, 2005. 68 p.Biblioteca(s): Embrapa Amazônia Oriental. |
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Registros recuperados : 3 | |
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