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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
06/07/2022 |
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Data da última atualização: |
06/09/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
NICKEL, O.; FAJARDO, T. V. M.; TARGON, M. L. N. P.; MACHADO, M. A. |
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Afiliação: |
OSMAR NICKEL, CNPUV; THOR VINICIUS MARTINS FAJARDO, CNPUV; M. L. N. P. TARGON, Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira; M. A. MACHADO, Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira. |
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Título: |
Production and Use of Polyclonal Antibodies to the Coat Protein of Apple stem grooving virus expressed in Escherichia coli. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Acta Horticulturae, v. 657, p. 35-40, ISHS 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
The coat protein (cp) gene of Apple stem grooving Capillovirus was amplified by RT-PCR, cloned, sequenced and subcloned in the expression plasmid pMal-c2. The ASGV cp gene was expressed in E. coli as a fusion protein (fp) containing a fragment of E. coli maltose binding protein (MBP). Bacterial cells were disrupted by sonication and the ASGVcp/MBP-fp was purified by amylose resin affinity chromatography. Polyclonal antibodies from rabbits immunized with the fp, gave specific reactions to ASGV from several infected apple cultivars at dilutions of up to 1:2000 in indirect ELISA. The ASGVcp/MBP-fp reacted to antisera raised against it as well as to commercial antisera against ASGV virions in immunoblotting. These results are a fundamental contribution to the development of an efficient ASGVindexing of propagative and mother stock materials in Southern Brazil. |
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Palavras-Chave: |
ASGV; Recombinant viral antigen. |
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Thesagro: |
Elisa. |
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Thesaurus Nal: |
Antibodies; Apples; Recombinant antigens. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144491/1/Nickel-ISHS-657-2003-p35-40.pdf
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Marc: |
LEADER 01541nam a2200217 a 4500
001 2144491
005 2023-09-06
008 2004 bl uuuu u00u1 u #d
100 1 $aNICKEL, O.
245 $aProduction and Use of Polyclonal Antibodies to the Coat Protein of Apple stem grooving virus expressed in Escherichia coli.$h[electronic resource]
260 $aActa Horticulturae, v. 657, p. 35-40, ISHS 2004.$c2004
520 $aThe coat protein (cp) gene of Apple stem grooving Capillovirus was amplified by RT-PCR, cloned, sequenced and subcloned in the expression plasmid pMal-c2. The ASGV cp gene was expressed in E. coli as a fusion protein (fp) containing a fragment of E. coli maltose binding protein (MBP). Bacterial cells were disrupted by sonication and the ASGVcp/MBP-fp was purified by amylose resin affinity chromatography. Polyclonal antibodies from rabbits immunized with the fp, gave specific reactions to ASGV from several infected apple cultivars at dilutions of up to 1:2000 in indirect ELISA. The ASGVcp/MBP-fp reacted to antisera raised against it as well as to commercial antisera against ASGV virions in immunoblotting. These results are a fundamental contribution to the development of an efficient ASGVindexing of propagative and mother stock materials in Southern Brazil.
650 $aAntibodies
650 $aApples
650 $aRecombinant antigens
650 $aElisa
653 $aASGV
653 $aRecombinant viral antigen
700 1 $aFAJARDO, T. V. M.
700 1 $aTARGON, M. L. N. P.
700 1 $aMACHADO, M. A.
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Embrapa Uva e Vinho (CNPUV) |
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